Alexa Fluor™ 594 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 594 NHS Ester (Succinimidyl Ester)
Invitrogen™

Alexa Fluor™ 594 NHS Ester (Succinimidyl Ester)

아민 반응성 isomeric 혼합물인 Alexa Fluor 594 carboxylic acid로 만들어지는 conjugate succinimidyl ester는 녹색 형광 conjugate와 스펙트럼 중복이 거의 없으며자세히 알아보기
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카탈로그 번호수량
A201045 mg
A375723 x 100 μg
A200041 mg
A3756525 mg
카탈로그 번호 A20104
제품 가격(KRW)
2,140,000
온라인 행사
Ends: 31-Dec-2025
2,674,000
할인액 534,000 (20%)
Each
카트에 추가하기
수량:
5 mg
제품 가격(KRW)
2,140,000
온라인 행사
Ends: 31-Dec-2025
2,674,000
할인액 534,000 (20%)
Each
카트에 추가하기
아민 반응성 isomeric 혼합물인 Alexa Fluor 594 carboxylic acid로 만들어지는 conjugate succinimidyl ester는 녹색 형광 conjugate와 스펙트럼 중복이 거의 없으며 Ar-Kr 레이저의 568 nm, 오렌지색 He-Ne 레이저의 594 nm까지 효율적으로 여기됩니다. Texas Red dye와 분광학적으로 유사한 Alexa Fluor 594 dye와 그 conjugate는 밝은 형광을 발하며 광안정성이 높습니다. 5 mg unit 크기로도 구입할 수 있습니다(카탈로그 번호: A-20104).
For Research Use Only. Not for use in diagnostic procedures.
사양
화학물질 반응성Amine
방출617 nm
여기590 nm
라벨 또는 염료Alexa Fluor™ 594
제품 유형Dye
수량5 mg
반응성 부분Active Ester, Succinimidyl Ester
배송 조건Room Temperature
라벨 유형Alexa Fluor
제품라인Alexa Fluor
Unit SizeEach
구성 및 보관
Store in freezer (-5 to -30°C) and protect from light.

자주 묻는 질문(FAQ)

I am labeling a protein with Alexa Fluor 488 SDP ester. The manual recommends using a sodium bicarbonate buffer at pH 8.3. Can I use a different buffer instead?

Yes. The important thing is to use a buffered solution with a pH between 8.0 and 8.5. Do not use Tris buffer, which has amine groups. Most other buffers will work fine in that pH range. This is also true for other amine-reactive dyes, such as succinimidyl (NHS) esters or TFP esters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am not going to use all of my Alexa Fluor succinimidyl ester reactive dye. Can I just make it up in DMSO and store aliquots at -20 degrees C?

This is not recommended. Any trace amounts of water in the DMSO can promote spontaneous hydrolysis over time. Even if using anhydrous DMSO, DMSO is hygroscopic; it readily absorbs moisture from the atmosphere over time. A better alternative is to dissolve the reactive dye in a volatile solvent, make smaller aliquots and then evaporate off the solvent using a vacuum pump. The smaller aliquots of solid reactive dye should then be stored frozen, desiccated and protected from light. Contact Technical Support by sending an email to techsupport@thermofisher.com for the recommended volatile solvent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (54)

인용 및 참조 문헌
Abstract
PKC-induced intracellular trafficking of Ca(V)2 precedes its rapid recruitment to the plasma membrane.
Authors:Zhang Y, Helm JS, Senatore A, Spafford JD, Kaczmarek LK, Jonas EA,
Journal:J Neurosci
PubMed ID:18322103
'Activation of protein kinase C (PKC) potentiates secretion in Aplysia peptidergic neurons, in part by inducing new sites for peptide release at growth cone terminals. The mechanisms by which ion channels are trafficked to such sites are, however, not well understood. We now show that PKC activation rapidly recruits new ... More
Development of a novel FRET immunosensor technique.
Authors:Lichlyter DJ, Grant SA, Soykan O
Journal:Biosens Bioelectron
PubMed ID:14611757
'We report on a novel technique to develop an optical immunosensor based on fluorescence resonance energy transfer (FRET). IgG antibodies were labeled with acceptor fluorophores while one of three carrier molecules (protein A, protein G, or F(ab'')2 fragment) was labeled with donor fluorophores. The carrier molecule was incubated with the ... More
A lipid receptor sorts polyomavirus from the endolysosome to the endoplasmic reticulum to cause infection.
Authors:Qian M, Cai D, Verhey KJ, Tsai B,
Journal:PLoS Pathog
PubMed ID:19503604
'The mechanisms by which receptors guide intracellular virus transport are poorly characterized. The murine polyomavirus (Py) binds to the lipid receptor ganglioside GD1a and traffics to the endoplasmic reticulum (ER) where it enters the cytosol and then the nucleus to initiate infection. How Py reaches the ER is unclear. We ... More
Sensitive fluorescence polarization technique for rapid screening of alpha-synuclein oligomerization/fibrillization inhibitors.
Authors:Luk KC, Hyde EG, Trojanowski JQ, Lee VM,
Journal:Biochemistry
PubMed ID:17927212
'Parkinson''s disease (PD) is characterized by the accumulation of fibrillar alpha-synuclein (alpha-Syn) inclusions known as Lewy bodies (LBs) and Lewy neurites. Mutations in the alpha-Syn gene or extra copies thereof cause familial PD or dementia with LBs (DLB) in rare kindreds, but abnormal accumulations of wildtype alpha-Syn also are implicated ... More
Persistent cAMP-signals triggered by internalized G-protein-coupled receptors.
Authors:Calebiro D, Nikolaev VO, Gagliani MC, de Filippis T, Dees C, Tacchetti C, Persani L, Lohse MJ,
Journal:PLoS Biol
PubMed ID:19688034
'G-protein-coupled receptors (GPCRs) are generally thought to signal to second messengers like cyclic AMP (cAMP) from the cell surface and to become internalized upon repeated or prolonged stimulation. Once internalized, they are supposed to stop signaling to second messengers but may trigger nonclassical signals such as mitogen-activated protein kinase (MAPK) ... More