Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit
Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit
Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit
Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit
Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit
인용 및 참조 문헌 (62)
Invitrogen™

Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit

Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit에는 hydrogen peroxide (H2O2) 또는 peroxidase 활성 검출에 Amplex™ Red reagent (10-acetyl-3,7-dihydroxyphenoxazine)을 사용하는 민감한 one-step자세히 알아보기
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카탈로그 번호수량
A22188500 Assays
카탈로그 번호 A22188
제품 가격(KRW)
585,000
Online offer
Ends: 31-Dec-2025
650,000
할인액 65,000 (10%)
Each
카트에 추가하기
수량:
500 Assays
제품 가격(KRW)
585,000
Online offer
Ends: 31-Dec-2025
650,000
할인액 65,000 (10%)
Each
카트에 추가하기
Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit에는 hydrogen peroxide (H2O2) 또는 peroxidase 활성 검출에 Amplex™ Red reagent (10-acetyl-3,7-dihydroxyphenoxazine)을 사용하는 민감한 one-step assay가 들어있습니다. Amplex™ Red reagent와 horseradish peroxidase (HRP)를 병용하여 세포 등 생물 샘플에서 분비되거나 효소 결합 반응에서 생성되는 H2O2를 검출하는데 사용하고 있습니다. 또한 Amplex™ Red reagent은 H2O2이 과다한 경우에 있어 매우 민감한 peroxidase 활성 분석으로 사용할 수 있습니다. peroxidase가 있으면 Amplex™ Red reagent이 H2O2에 1:1 stoichiometry로 반응해 적색 형광 산화물인 resorufin을 생성합니다. Resorufin의 최대 여기 및 방출 파장은 각각 571 nm, 585 nm로 흡광 계수가 높아(58,000 ± 5,000 cm–1M–1), 형광분석 또는 분광측정 검사를 수행할 수 있습니다. 이 반응은 100 μL 용량에서 10 picomole 만큼 미량의 H2O2또는 HRP 1 x 10–5 U/mL 검출에 사용됩니다.

Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit 사양:

• 반응 산물 resorufin의 Ex/Em: ∼571/585 nm
• hydrogen peroxide 또는 peroxidase in vitro 검출에 사용하는 민감한 one-step assay
• 분석양 100 μL에서 horseradish peroxidase 활성 10 μU/mL 또는 hydrogen peroxide 10 picomole 검출
• 분석 당 반응양 100 μL로 500회 분석에 충분한 시약 제공.
• 96-well 형광 또는 흡광 microplate reader에 맞게 설계되었으나 다른 용량, 형광분석기, 분광분석계에도 쉽게 적용할 수 있습니다.

Oxidases 기질에 대한 자세한 정보
이들 제품에 대한 더 많은 정보는 Molecular Probes™ Handbook의Substrates for Oxidases, Including Amplex Red Kits—Section 10.5을 참조하십시오.

이 제품은 연구용으로만 사용가능합니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법Fluorescence
염료 유형Other Label(s) or Dye(s)
형식Tube(s), 96-well plate
수량500 Assays
배송 조건Room Temperature
용도(애플리케이션)Hydrogen Peroxidase Assay
용도 (장비)Microplate Reader, Spectrophotometer, Fluorometer
제품라인Amplex
제품 유형Amplex Red Assay Kit
Unit SizeEach
구성 및 보관
Store in freezer -5°C to -30°C and protect from light.

자주 묻는 질문(FAQ)

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H2O2 oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit (Cat. No. A22188) to measure H2O2 from frozen cell culture supernatant?

We recommend using fresh samples that have not been frozen since H2O2 degrades over time and more readily in diluted form or in solutions that contain metal ions or natural antioxidants. For assaying cell culture supernatant, we would recommend analyzing samples as soon as possible.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can Amplex Red Assays be performed using cell lysates?

This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H2O2 required for the reaction, competing with HRP (and catalase) for the substrate.

The Amplex Red Assays are best performed with either purified enzymes or extracted H2O2 in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (62)

인용 및 참조 문헌
Abstract
Roles for the human ATP-dependent Lon protease in mitochondrial DNA maintenance.
Authors:Lu B,Yadav S,Shah PG,Liu T,Tian B,Pukszta S,Villaluna N,Kutejová E,Newlon CS,Santos JH,Suzuki CK
Journal:The Journal of biological chemistry
PubMed ID:17420247
Similar transition states mediate the Q-cycle and superoxide production by the cytochrome bc1 complex.
Authors:Forquer I,Covian R,Bowman MK,Trumpower BL,Kramer DM
Journal:The Journal of biological chemistry
PubMed ID:17008316
Overexpression of Na(+)/K (+)-ATPase parallels the increase in sodium transport and potassium recycling in an in vitro model of proximal tubule cellular ageing.
Authors:Silva E, Gomes P, Soares-da-Silva P,
Journal:J Membr Biol
PubMed ID:17334838
'Na(+)/K(+)-ATPase plays a key role in the transport of Na(+) throughout the nephron, but ageing appears to be accompanied by changes in the regulation and localization of the pump. In the present study, we examined the effect of in vitro cell ageing on the transport of Na(+) and K(+) ions ... More
Reactive oxygen species production via NADPH oxidase mediates TGF-beta-induced cytoskeletal alterations in endothelial cells.
Authors:Hu T, Ramachandrarao SP, Siva S, Valancius C, Zhu Y, Mahadev K, Toh I, Goldstein BJ, Woolkalis M, Sharma K
Journal:Am J Physiol Renal Physiol
PubMed ID:16159901
'Cytoskeletal alterations in endothelial cells have been linked to nitric oxide generation and cell-cell interactions. Transforming growth factor (TGF)-beta has been described to affect cytoskeletal rearrangement in numerous cell types; however, the underlying pathway is unclear. In the present study, we found that human umbilical vein endothelial cells (HUVEC) have ... More
Subcellular localization of Nox4 and regulation in diabetes.
Authors:Block K, Gorin Y, Abboud HE,
Journal:Proc Natl Acad Sci U S A
PubMed ID:19706525
'Oxidative stress is implicated in human diseases. Some of the oxidative pathways are harbored in the mitochondria. NAD(P)H oxidases have been identified not only in phagocytic but also in somatic cells. Nox4 is the most ubiquitous of these oxidases and is a major source of reactive oxygen species (ROS) in ... More
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