Mouse (ICR) Inactivated Embryonic Fibroblasts
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Mouse (ICR) Inactivated Embryonic Fibroblasts
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Mouse (ICR) Inactivated Embryonic Fibroblasts

Mouse embryonic fibroblasts (MEFs) are used as a feeder layer when culturing mouse and human embryonic stem cells (ESCs) and자세히 알아보기
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카탈로그 번호수량
A249031 mL
카탈로그 번호 A24903
제품 가격(KRW)
81,000
Online offer
Ends: 31-Dec-2025
89,000
할인액 8,000 (9%)
Each
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수량:
1 mL
제품 가격(KRW)
81,000
Online offer
Ends: 31-Dec-2025
89,000
할인액 8,000 (9%)
Each
카트에 추가하기
Mouse embryonic fibroblasts (MEFs) are used as a feeder layer when culturing mouse and human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). They are isolated from mouse (strain ICR) embryos. The cells are expanded and inactivated by γ irradiation and cryopreserved. Prior to cryopreservation, the cells are tested for viability (>80% post-thaw viability), verified for growth arrest by plating inactivated cells at low density and monitoring for foci of cell growth after 14 days in culture, and also tested for their ability to support growth of mouse embryonic stem cells (mESCs), human embryonic stem cells (hESCs), and marker expression (Oct 4).
For Research Use Only. Not for use in diagnostic procedures.
사양
공여자 소스Pooled
제품 유형Mouse Embryonic Fibroblast
수량1 mL
AgeFetal
셀 유형Feeder Cells (Fibroblast)
Culture TypeFeeder Dependent
형태Cryopreserved
종Mouse
Unit SizeEach
구성 및 보관
Store in liquid nitrogen.

자주 묻는 질문(FAQ)

What is the difference between Mouse (ICR) Inactivated Embryonic Fibroblasts (Cat. No. A24903) and CF1 Mouse Embryonic Fibroblasts, irradiated (Cat. Nos. A34180, A34181)? Can they be used to reprogram fibroblasts?

Mouse (ICR) Inactivated Embryonic Fibroblasts and CF1 Mouse Embryonic Fibroblasts, irradiated are inactivated feeder layer fibroblast cells that are isolated from different mouse strains. Researchers often have a preference for one or the other. Neither of these can be used to reprogram fibroblasts.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does the culture vessel need to be coated prior to seeding Mouse (ICR) Inactivated Fibroblasts?

Yes, the cutlure vessel needs to be coated with Attachment Factor Protein (Cat. No. S006100) at 37 degrees C for 30 min or at room temperature for 2 hours. The coated vessels can be used immediately or stored at room temperature for up to 24 hours.

What is the sex of mouse embryros from which Mouse (ICR) Inactivated Fibroblasts are isolated?

We do not determine the sex of the embryos. The embryos were pooled from several litters which are a mixture of male and female.