MagMAX™ mirVana™ Total RNA Isolation Kit
MagMAX&trade; <i>mir</i>Vana&trade; Total RNA Isolation Kit
Applied Biosystems™

MagMAX™ mirVana™ Total RNA Isolation Kit

Scalable isolation of RNA (including small RNAs)The MagMAX™ mirVana™ Total RNA Isolation Kit is designed for isolation of total RNA,자세히 알아보기
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카탈로그 번호수량
A2782896 reactions
카탈로그 번호 A27828
제품 가격(KRW)
872,000
Online offer
Ends: 31-Dec-2025
917,000
할인액 45,000 (5%)
Each
카트에 추가하기
수량:
96 reactions
제품 가격(KRW)
872,000
Online offer
Ends: 31-Dec-2025
917,000
할인액 45,000 (5%)
Each
카트에 추가하기
Scalable isolation of RNA (including small RNAs)
The MagMAX™ mirVana™ Total RNA Isolation Kit is designed for isolation of total RNA, including small RNAs such as microRNAs, from a wide variety of sample types. The kit uses MagMAX magnetic-bead technology, enabling reproducible recovery of high-quality RNA that is suitable for a broad range of applications, including TaqMan™ miRNA Detection Assays.

The magnetic bead–based purification format allows you to easily scale from processing 6 to 96 samples at once when used with the KingFisher™ Duo Prime or KingFisher™ Flex Magnetic Particle Processor. Alternatively, samples can be processed manually with the use of a magnetic stand.

Features of the MagMAX mirVana Total RNA Isolation Kit include:
• Automation-ready, phenol-free extraction
• Streamlined protocols for numerous non-invasive biological samples as well as tissues and cell culture
• Recovery of pure miRNAs compatible with miRNA-Seq and qRT-PCR methods such as the TaqMan™ Advanced miRNA cDNA Synthesis Kit and TaqMan™ Advanced miRNA Assays

One kit to isolate true total RNA from a diverse set of sample types
Why purchase multiple purification kits to isolate all RNAs present in your different sample types? The MagMAX mirVana Total RNA Isolation Kit eliminates the headache of ordering “specialized” kits because it has been optimized for use with most biological samples. No longer are separate, specific kits needed to isolate microRNAs from plasma and solid tissue samples.

The kit is designed for both low- and high-throughput isolation of true total RNA that covers the full range of RNAs present in cells from small RNAs such as microRNAs to non-coding RNA, messenger RNA, and ribosomal RNA. The phenol-free, magnetic-bead format makes the kit fully amenable to automated RNA isolation from the following samples:

• Serum/plasma
• Whole blood
• Tissue
• Cell culture
• Urine

Benefits of MagMAX magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating total RNA. Beads bind RNA more efficiently than glass fiber filters, thereby enabling higher and more consistent yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples.
For research use only. Not for use in diagnostic procedures.
사양
용리량20 to 100 μL
최종 제품 유형Total RNA
용도(애플리케이션)RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
용도(장비)KingFisher™ Duo Prime, KingFisher™ Flex, KingFisher™ Apex
고처리량 호환성High-throughput Compatible
정제 시간Up to 1.5 hr.
수량96 reactions
배송 조건Box 1: Dry Ice
Box 2: Room Temperature
시작 물질 양Cells: ≤106
Plasma: ≤100 μL
Serum: ≤100 μL
Tissue: ≤50 mg
Urine: ≤250 μL
Whole Blood: ≤50 μL
수율Cells: ≤32 μg RNA per 50,000 cells
Tissue: ≤500 μg per 5 mg
Isolation TechnologyMagnetic Bead
샘플 종류Cells, Plasma, Serum, Tissue, Urine, Whole Blood
TargetmRNA, siRNA, miRNA
Unit SizeEach
구성 및 보관

Box 1 (store at -15 to -25°C)
• 1 x 0.48 mL Proteinase K, 20 mg/mL
• 1 x 0.96 mL Lysis/Binding Enhancer
• 1 x 0.2 mL TURBO DNase, 20 U/uL

Box 2 (store at room temperature)
• 1 x 115 mL Lysis Buffer
• 1 x 4.4 mL PK Digestion Buffer
• 1 x 2 mL RNA Binding Beads
• 1 x 20 mL Wash Solution 1 Concentrate
• 1 x 60 mL Wash Solution 2 Concentrate
• 1 x 4.8 mL Rebinding Buffer
• 1 x 4.8 mL MagMAX TURBO DNase Buffer
• 1 x 9.6 mL Elution Buffer
• 1 Processing Plate
• 2 Elution Plates
• 4 Plate Covers

자주 묻는 질문(FAQ)

Can I purchase the RNA Binding beads from the MagMAX MirVana Total RNA Isolation Kit (Cat. No. A27828) separately?

Yes, you can. The DNA Binding Beads for MagMAX-96 DNA Multi-Sample Kit (Cat. No. 4489112) are the same as that used in A27828.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the minimum cell number for RNA isolation using MagMAX mirVana Total RNA Isolation Kit (Cat. No. A27828)?

We have tested extraction from as low as 1,000 cells using cultured HeLa, Jurkat, and HepG2 cells.

What is the level of detection for somatic variants with the Oncomine Myeloid Research Assay GX?

Detection of somatic variants with the Oncomine Myeloid Research Assay GX has been verified down to 5% allele frequency.

Which extraction kits are compatible with Oncomine Myeloid Research Assay GX?

Here is a list of kits that are compatible with Oncomine Myeloid Research Assay GX:

Nucleic acid isolation - RNA samples
- MagMAX mirVana Total RNA Isolation Kit, Cat. No. A27828
- PureLink Total RNA Blood Kit, Cat. No. K156001
- RNaseZap RNase Decontamination Solution, Cat. No. AM9780, AM9782, AM9784

Nucleic acid isolation - DNA samples
- MagMAX DNA Multi-Sample Ultra 2.0 Kit, Cat. No. A36570
- PureLink Genomic DNA Mini Kit, Cat. No. K1820-01

What are the sample input requirements for Oncomine Myeloid Research Assay GX?

The recommended minimum sample input is 27.75 ng of DNA (1.11 ng/µL minimum concentration) and 14.25 ng of RNA (0.95 ng/µL minimum concentration).