The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit (Cat. No. A29133). It is자세히 알아보기
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A29129
For 1 L of culture
A29130
For 10 L of culture
A29131
For 50 L of culture
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카탈로그 번호 A29129
제품 가격(KRW)
1,109,000
Each
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For 1 L of culture
제품 가격(KRW)
1,109,000
Each
카트에 추가하기
The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit (Cat. No. A29133). It is designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. The high-efficiency, cationic transfection reagent, coupled with an optimized transfection enhancer and culture feed, will power the highest possible level of protein expression from ExpiCHO-S™ Cells (Cat. No. A29127) cultured in ExpiCHO™ Expression Medium (Cat. No. A2910001).
The ExpiFectamine CHO Transfection Kit: • Achieves greater than 90% transfection efficiency of high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression • Requires one-third to one-half less plasmid DNA than other transfection reagents while achieving superior expression performance • Enables options for three transient expression protocols for maximal work flexibility while still fitting into the same overall workflow used by other transient expression systems, such as FreeStyle™ MAX CHO and Expi293™ • Delivers robust and reproducible transfection results, giving you greater confidence in your results • Demonstrates linear scalability of transfections from culture volumes of <1 mL to >10 L
The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO™ Expression System Kit. The transfection kit supplies the cationic ExpiFectamine™ CHO Transfection Reagent, ExpiFectamine™ CHO Transfection Enhancer, and ExpiCHO Feed.
본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
Kit includes: • ExpiFectamine™ CHO Reagent, 3.2 mL • ExpiFectamine™ CHO Enhancer, 6 mL • ExpiCHO Feed, 330 mL
Sufficient for transfection of 1 L of culture Store in the dark at 2°C to 8°C.
자주 묻는 질문(FAQ)
My ExpiFectamine CHO Transfection Kit (Catalog No. A29130) was accidentally frozen. Can I still use it?
ExpiFectamine reagents generally does not transfect well after being frozen, as the freezing disrupts the lipid micelles. Therefore, we do not recommend using this kit.
Do you offer the ExpiFectamine CHO Transfection Enhancer, from the ExpiFectamine CHO Transfection Kit, as a standalone product?
No, we do not offer this ExpiFectamine CHO Transfection Kit component as a standalone product.
How many days should I wait before harvesting my protein with the ExpiCHO system?
Optimal time to harvest protein will depend on the specific properties of the protein being expressed and the protocol chosen. Typical harvest times to reach maximum titers for the various protocols are as follows:
- Standard Protocol: 8-10 days post-transfection
- High Titer Protocol: 10-12 days post-transfection
- Max Titer Protocol: 12-14 days post-transfection
I didn't add the enhancer with the ExpiCHO system. Will I still get good protein expression?
The Enhancer is designed to work with the ExpiCHO system for maximal expression. If the enhancer is not added, there will be a greater than 50% loss in expression.
Can I use a different transfection reagent with the Expi-CHO Expression System?
The ExpiFectamine CHO Transfection Reagent, ExpiFectamine CHO Enhancer and ExpiCHO Feed are optimized to work together to provide maximal protein expression levels and are provided as a single kit for convenience. The ExpiFectamine CHO Transfection Reagent provides high transfection efficiency of high density cultures, superior to any other transfection reagent. Expression levels greater than 30-fold higher are obtained using the ExpiCHO kit as directed versus substitution of PEI as a transfection reagent, while also using 50% less DNA.