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Gibco™
ExpiCHO™ Expression System Kit
The ExpiCHO Expression System Kit combines the power of rapid, ultra-high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells.
| 카탈로그 번호 | 세포주 |
|---|---|
| A29133 | CHO-S, SFM Adapted |
카탈로그 번호 A29133
제품 가격(KRW)
2,874,000
キャンペーン価格
Ends: 31-Dec-2025
3,025,000할인액 151,000 (5%)
Each
세포주:
CHO-S, SFM Adapted
제품 가격(KRW)
2,874,000
キャンペーン価格
Ends: 31-Dec-2025
3,025,000할인액 151,000 (5%)
Each
The ExpiCHO Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells. It is based on high-density culture of ExpiCHO-S cells in ExpiCHO Expression Medium. Transient expression is powered by ExpiFectamine CHO transfection reagent, designed specifically for high-density CHO suspension culture, in combination with a specialized transfection enhancer and culture feed.
All components work together synergistically with expression yields of 1–3 g per L of transfected culture demonstrated for some antibody and non-antibody proteins.
The ExpiCHO Expression System enables:
- Milligram to multigram yields of recombinant protein per liter of culture in 5–14 days post-transfection
- Robust and reproducible transfection results, for greater confidence in results and ability to use CHO cells from research to discovery
- Transfection efficiency of > 90% for high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
- Use of one-third to one-half less plasmid DNA than other transfection reagents
- Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows
- Scalable expression for culture formats from <1 mL in deep well plates up to 5 L when using Nalgene angled-bottom shake flasks
The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO SFM, and an antibody-expressing positive control vector.
ExpiCHO-S cells, ExpiCHO Expression Medium, ExpiFectamine CHO Transfection Kit, and OptiPRO SFM may also be purchased separately.
All components of the system are animal origin–free.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포주CHO-S, SFM Adapted
배달 유형Transfection
용도(애플리케이션)Protein Production, Constitutive Expression
제품라인ExpiCHO
단백질 태그Untagged
수량1 kit
배송 조건Approved for shipment at Room Temperature or on Wet or Dry Ice
발현 시스템Mammalian
프로모터CMV
유형Expression System
Unit SizeEach
구성 및 보관
• 2 vials frozen ExpiCHO-S cells
• 1000 mL ExpiCHO Expression Medium
• 1 ExpiFectamine CHO Transfection Kit for 1 L of culture
• 1 vial Antibody Expressing Positive Control Vector
• 100 mL OptiPRO SFM
Store all components at 2-8°C, except frozen cells which should be stored in liquid nitrogen.
• 1000 mL ExpiCHO Expression Medium
• 1 ExpiFectamine CHO Transfection Kit for 1 L of culture
• 1 vial Antibody Expressing Positive Control Vector
• 100 mL OptiPRO SFM
Store all components at 2-8°C, except frozen cells which should be stored in liquid nitrogen.