TaqMan™ RNase P Assay, VIC™ dye/QSY™ probe, primer-limited

The Applied Biosystems™ TaqMan™ RNase P Assay with VIC™ dye-labeled QSY™ probe provides a pre-formulated assay for quantitating human RNase P. This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. It consists of a VIC dye-labeled QSY probe plus sequence-specific forward and reverse primers. The assay can be used for multiplex or singleplex PCR reactions and it is primer limited, so it can be used with samples that overexpress RNase P. For a primer-limited assay with ABY™ dye-labeled probe rather than VIC dye-labeled probe, see Cat. No. 4485715.

Benefits of this assay include:

• Eliminate assay design time using pre-designed primers and pre-designed VIC dye-labeled TaqMan probe
• Minimize experimental optimization time
• Use in conjunction with FAM™ dye-labeled predesigned gene expression assays
• Formulated at a primer-limited concentration for use in multiplex reactions

Save time in assay design, formulation, and testing by using the TaqMan RNase P Assay as your control when quantifying gene expression. This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.

In order to facilitate multiplex experiments, the assay formulation is primer limited, allowing the assay to be used with high RNase P expressors.

The VIC dye is optimized for use with the second filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments.