Silencer™ Select Human DNA Damage Response siRNA Library

DNA surveillance proteins monitor DNA integrity, and they can activate cell cycle checkpoints and DNA repair pathways in response to DNA damage. This can prevent potentially deleterious mutations. The Silencer™ Select Human DNA Damage Response siRNA Library contains 1,746 Ambion™ Silencer Select highly potent and chemically-modified siRNAs targeting 582 DNA damage response genes. This library was designed using the most up-to-date genome databases, including the NCBI RefSeq database and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC). The Silencer Select siRNAs are designed using Thermo Fisher Scientific's leading algorithms—along with our novel locked nucleic acid (LNA) chemical modifications, this helps maximize efficacy, specificity and potency. The Silencer Select Human DNA Damage Response siRNA Library is an excellent option for RNAi screening against cellular pathways that sense, signal and repair DNA lesions following DNA damage.

• Superior efficacy—Reduce off target effects by up to 90% due to latest design algorithms and LNA chemical modifications
• Highest efficiency —Perform knockdown studies with 100-fold more potent siRNAs than currently available siRNAs
• Dependable —Achieve faster discoveries via consistency of gene knockdown and reliability of phenotypic results
• Convenient—Reduce data de-convolution and accelerate target validation with our arrayed format
• Customizable—Choose the best option for your workflow demands, including additional siRNA scales and pooled formats

Why use the Silencer Select Human DNA Damage Response siRNA Library

Together with careful bioinformatics selection of gene targets, Silencer Select siRNAs are optimized with latest design algorithms, proprietary chemical modifications and high quality synthesis to help ensure desired RNAi outcomes. The Silencer Select Human DNA Damage Response siRNA Library contains our pre-designed and when available, validated siRNAs generated with highest potency and specificity in mind so you can focus on the highest data quality backed by the best in industry guarantee.

Silencer Select siRNA libraries empower you with highly potent siRNAs to maximize discovery with even less

Ambion Silencer Select siRNAs are designed with the highest potency of available siRNAs, resulting in 100-fold more potent siRNAs and allowing gene knockdowns at the lowest concentrations (see figure below). The highly potent Silencer Select siRNAs allow you to use even lower concentrations of siRNAs, which helps to minimize off target effects, reduce false negative data and maximize siRNA library output for additional screens and data validation. The Silencer Select Human DNA Damage Response siRNA Library contains 0.25 nmol of each highly potent siRNA, sufficient for at least 500 transfections (at 5 nM siRNA, 100 μL transfection volume).

Flexibility to meet your workflow needs

Thermo Fisher Scientific offers comprehensive list of pre-defined or custom siRNA libraries to human, mouse or rat genes with 1, 2, or 5 nmol of each siRNA or larger sizes. For a complete gene list, additional information or to discuss your research with one of our technical experts, contact your local sales representative or e-mail us at RNAilibraries@lifetech.com.