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인용 및 참조 문헌 (6)
Invitrogen™
Violet Ratiometric Membrane Asymmetry Probe/Dead Cell Apoptosis Kit, for flow cytometry
The Violet Ratiometric Membrane Asymmetry Probe/Dead Cell Apoptosis Kit provides a simple and fast method for the detection of apoptosis자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| A35137 | 1 kit |
카탈로그 번호 A35137
제품 가격(KRW)견적 요청하기
-
수량:
1 kit
The Violet Ratiometric Membrane Asymmetry Probe/Dead Cell Apoptosis Kit provides a simple and fast method for the detection of apoptosis with dead cell discrimination by flow cytometry. The Violet Ratiometric Membrane Asymmetry Probe, 4'-N,N-diethylamino-6-(N,N,N-dodecyl-methylamino-sulfopropyl)-methyl-3-hydroxyflavone (F2N12S), is a novel violet excitable dye for the detection of membrane asymmetry changes during apoptosis. The dye exhibits an excited-state intramolecular proton transfer (ESIPT) reaction resulting in a dual fluorescence with two emission bands corresponding to 530 nm and 585 nm, producing a two-color ratiometric response to variations in surface charge. Ratiometric probes have several advantages over traditional fluorochrome labeled reagents. The ratiometric probe is a self-calibrating absolute parameter of apoptotic transformation, which is independent of probe concentration, cell size, and instrument variation, such as fluctuations of laser intensity or sensitivity of the detectors. Given that apoptosis modifies the surface charge of the outer leaflet of the plasma membrane the violet membrane asymmetry probe F2N12S can monitor changes in membrane asymmetry that occur during apoptosis through a change in the relative intensity of the two emission bands of the dye. The F2N12S probe is combined with SYTOX(R) AADvanced dead cell stain, which is capable of passing through the cell membrane only in late apoptotic or necrotic cells allowing discrimination form early apoptotic cells. Samples can be analyzed after a 5 minute incubation at room temperature and does not require special buffers or wash steps. This kit can be paired with other reagents such as MitoProbe™ DiIC1(5) or annexin V for multiparametric analysis of apoptosis and viability. This reagent kit allow researchers to maximaize the utility of their instruments by utilizing the violet laser Each kit contains sufficient reagents for ∼100 flow cytometry tests.
View a selection guide for all apoptosis assays for flow cytometry.
View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.
사양
여기/방출SYTOX™ AADvanced™: 546/647
F2N12S: 405/530
F2N12S: 405/530
유세포분석기 레이저 라인405, 488
용도(애플리케이션)Flow Cytometry
용도(장비)Flow Cytometer
라벨 유형Other Label(s) or Dye(s)
라벨 또는 염료F2N12S, SYTOX AADvanced Dead Cell Stain
반응 수100
제품 유형Dead Cell Apoptosis Kit
수량1 kit
배송 조건Dry Ice
검출 방법Fluorescence
형식Tube
Unit SizeEach
구성 및 보관
Contains 1 vial of F2N12S (100 μL), 1 vial of SYTOX™ AADvanced™ dead cell stain, and 1 vial of DMSO (200 μL).
Store at -20°C, dessicate and protect from light.
Store at -20°C, dessicate and protect from light.
인용 및 참조 문헌 (6)
인용 및 참조 문헌
Abstract
Visualization of lipid domains in giant unilamellar vesicles using an environment-sensitive membrane probe based on 3-hydroxyflavone.
Journal:Biochim Biophys Acta
PubMed ID:19027712
'We characterized the recently introduced environment-sensitive fluorescent membrane probe based on 3-hydroxyflavone, F2N12S, in model lipid membranes displaying liquid disordered (Ld) phase, liquid ordered (Lo) phase, or their coexistence. Steady-state fluorescence studies in large unilamellar vesicles show that the probe dual emission drastically changes with the lipid bilayer phase, which
Monitoring biophysical properties of lipid membranes by environment-sensitive fluorescent probes.
Journal:Biophys J
PubMed ID:19413953
We review the main trends in the development of fluorescence probes to obtain information about the structure, dynamics, and interactions in biomembranes. These probes are efficient for studying the microscopic analogs of viscosity, polarity, and hydration, as well as the molecular order, environment relaxation, and electrostatic potentials at the sites
Excited state proton transfer and solvent relaxation of a 3-hydroxyflavone probe in lipid bilayers.
Journal:J Phys Chem B
PubMed ID:18729506
The photophysics of a ratiometric fluorescent probe, N-[[4'- N, N-diethylamino-3-hydroxy-6-flavonyl]methyl]- N-methyl- N-(3-sulfopropyl)-1-dodecanaminium, inner salt (F2N12S), incorporated into phospholipid unilamellar vesicles is presented. The reconstructed time-resolved emission spectra (TRES) unravels a unique feature in the photophysics of this probe. TRES exhibit signatures of both an excited-state intramolecular proton transfer (ESIPT) and
Fluorescent biomembrane probe for ratiometric detection of apoptosis.
Journal:J Am Chem Soc
PubMed ID:17256940
Herein, we developed the first ratiometric fluorescent probe for apoptosis detection. This probe incorporates selectively into the outer leaflet of the cell plasma membrane and senses the loss of the plasma membrane asymmetry occurring during the early steps of apoptosis. The high specificity to the plasma membranes was achieved by
Death receptor 5 is activated by fucosylation in colon cancer cells.
Journal:FEBS J
PubMed ID:30589515
'The remarkable pro-apoptotic properties of tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) have led to considerable interest in this protein as a potential anticancer therapeutic. However, TRAIL is largely ineffective in inducing apoptosis in certain cancer cells, and the mechanisms underlying this selectivity are unknown. In colon adenocarcinomas, posttranslational modifications