High Select™ Depletion Spin Columns
High Select™ Depletion Spin Columns
High Select™ Depletion Spin Columns
High Select™ Depletion Spin Columns
Thermo Scientific™

High Select™ Depletion Spin Columns

Decrease albumin, immunoglobulins, and other high-abundance proteins from human serum or plasma in preparation for mass spectrometry analysis, or 1D and 2D gel electrophoresis using High Select depletion spin columns or resin.
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카탈로그 번호수량제품 유형정제 타겟
A363696 ColumnsMini Spin ColumnTop14 Abundant Protein
A363656 ColumnsMini Spin ColumnHSA/Immunoglobulin
A3636624 ColumnsMini Spin ColumnHSA/Immunoglobulin
A3636710 ColumnsMidi Spin ColumnHSA/Immunoglobulin
A3636850 mLResinHSA/Immunoglobulin
A3637110 ColumnsMidi Spin ColumnTop14 Abundant Protein
A3637250 mLResinTop14 Abundant Protein
A3637024 ColumnsMini Spin ColumnTop14 Abundant Protein
카탈로그 번호 A36369
제품 가격(KRW)
404,000
Online offer
Ends: 31-Dec-2025
475,000
할인액 71,000 (15%)
Each
카트에 추가하기
수량:
6 Columns
제품 유형:
Mini Spin Column
정제 타겟:
Top14 Abundant Protein
제품 가격(KRW)
404,000
Online offer
Ends: 31-Dec-2025
475,000
할인액 71,000 (15%)
Each
카트에 추가하기
Analysis of human fluids is often complicated by the presence of high concentrations of serum proteins, including albumin and IgG that can make up more than 70% of total serum protein. Removal of albumin, immunoglobulins, and other abundant proteins is essential for the study of low-abundance proteins. Thermo Scientific High Select HSA/Immunoglobulin and Top 14 Abundant Protein depletion spin columns and resins enable depletion of the most abundant proteins in human serum and plasma for improved detection of low-abundance proteins using mass spectrometry analysis or 1D or 2D gel electrophoresis.
• Flexible—resin provided in mini and midi column formats as well as bulk for custom filling options
• Optimized—removes >95% of IgG and >95% of albumin (HSA/Immunoglobulin); removes >95% of IgG and >95% of albumin, plus 12 other abundant proteins (Top 14)
• Fast—process samples in ∼10 minutes
• Economical—cost-effective spin columns are priced for single use
• Reduced carry-over—one-time use prevents protein carryover and experimental variability

High Select HSA/Immunoglobulin Depletion Resin uses highly specific, immobilized anti-HSA and anti-immuoglobulin antibodies (IgG, IgA, IgM, IgD, and IgE) to remove human serum albumin (HSA) and all major subclasses of immunoglobulins from serum and plasma. High Select Top14 Abundant Protein Depletion Resin uses highly specific immobilized antibodies to remove human serum albumin (HSA), albumin, IgG, IgA, IgM, IgD, IgE, kappa and lambda light chains, alpha-1-acidglycoprotein, alpha-1-antitrypsin, alpha-2-macroglobulin, apolipoprotein A1, fibrinogen, haptoglobin, and transferrin from serum, plasma, or spinal fluids. The resins are provided in an economical and convenient spin column format specifically designed for one-step processing and single use or bulk format for custom filling.

For Research Use Only. Not for use in diagnostic procedures.
사양
최종 제품 유형Protein
용도(장비)Mass Spectrometer
라벨 또는 염료Unlabeled
정제 타겟Top14 Abundant Protein
수량6 Columns
배송 조건Wet Ice
워크플로우 단계Protein Depletion
형식Mini Spin Column
제품라인High Select
제품 유형Mini Spin Column
시작 물질Plasma, Serum
Unit SizeEach
구성 및 보관
Store at 2–8°C.

자주 묻는 질문(FAQ)

Can I use the High Select Top14 Abundant Protein Depletion Midi Spin Columns to deplete proteins from rodent samples?

The High Select Top14 Abundant Protein Depletion Midi Spin Columns have only been validated for human samples. We do not recommend using them for protein depletion in rodent samples. For protein depletion in rodent samples, we recommend the following multi-species depletion resins:

  • CaptureSelect MultiSpecies Albumin Depletion Product (Cat. No. 191085305)
  • Pierce Albumin Serum Depletion Kits (Cat. No. 89875)


    • Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Are your High Select Top14 Abundant Protein Depletion Mini Spin Columns (Cat. No. A36369) compatible with cell culture media?

Our High Select Top14 Abundant Protein Depletion Mini Spin Columns have been tested and validated for protein depletion only on human plasma and serum, with each column optimized to bind up to 10 µL of sample.
We have not tested our product on cell culture media or with serum from different species, therefore we do not recommend using this product with cell culture media.

Is there a 96-well format available for the Top14 Abundant Protein Depletion Mini Spin Columns (Cat. No. A36369)?

No. The Top14 Abundant Protein Depletion Mini Spin Columns (Cat. No. A36369) do not come in a 96-well format. However, we do have a Top14 Abundant Protein Depletion Resin (Cat. No. A36372) that can be purchased as a stand-alone product.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

After depletion of abundant proteins from my sample, I still see albumin and other abundant proteins after LC-MS analysis. Did the depletion work?

Depletion does not remove all abundant proteins from the sample. Even with 99% depletion of a specific plasma protein, highly abundant proteins will still be the most abundant proteins in the sample detected by MS analysis. However, depletion significantly decreases the most abundant plasma proteins enabling identification of lower abundant proteins.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I proceed to downstream mass spec sample preparation after abundant plasma protein depletion?

After the depletion, samples are significantly diluted in buffer. Depleted samples should be concentrated by speed vac drying, solvent precipitation, or diafiltration. Samples dried using a speedvac are directly compatible with the EasyPep kit chemistry after resuspension using the Lysis Solution.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

View all High Select™ Depletion Spin Columns FAQs