Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester)

인용 및 참조 문헌 (7)

Invitrogen™

Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester)

Name: Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester)
SKU: A37566
Price: 7539000 KRW

무이성질체, 아민 반응성인 Alexa Fluor 647 carboxylic acid, succinimidyl ester dye와 conjugates는 Cy5 dye와 분광학적으로 동일하여 여기/방출 최대값이 ∼650/668 nm입니다.자세히 알아보기

보기 방식 변경

카탈로그 번호	수량
A37566	25 mg
A37573	3 x 100 μg
A20006	1 mg
A20106	5 mg

4 개 옵션

카탈로그 번호 A37566

제품 가격(KRW)

7,539,000

Each

카트에 추가하기

수량:

25 mg

제품 가격(KRW)

7,539,000

Each

카트에 추가하기

무이성질체, 아민 반응성인 Alexa Fluor 647 carboxylic acid, succinimidyl ester dye와 conjugates는 Cy5 dye와 분광학적으로 동일하여 여기/방출 최대값이 ∼650/668 nm입니다. 하지만 Alexa Fluor 647 conjugate는 Cy5 dye로 준비된 것보다 훨씬 밝습니다. A 5 mg 단위(A-20106)도 구매 가능합니다. 장파장 Alexa Fluor dye의 형광은 인간 눈에는 보이지 않지만 대부분의 영상 시스템으로는 쉽게 검출이 가능합니다.

For Research Use Only. Not for use in diagnostic procedures.

사양

방출672 nm

여기651 nm

제품 유형Dye

수량25 mg

배송 조건Room Temperature

제품라인Alexa Fluor

Unit SizeEach

구성 및 보관

1 tube
Store dessicated at -20°C, upon reciept

자주 묻는 질문(FAQ)

I am labeling a protein with Alexa Fluor 488 SDP ester. The manual recommends using a sodium bicarbonate buffer at pH 8.3. Can I use a different buffer instead?

Yes. The important thing is to use a buffered solution with a pH between 8.0 and 8.5. Do not use Tris buffer, which has amine groups. Most other buffers will work fine in that pH range. This is also true for other amine-reactive dyes, such as succinimidyl (NHS) esters or TFP esters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am not going to use all of my Alexa Fluor succinimidyl ester reactive dye. Can I just make it up in DMSO and store aliquots at -20 degrees C?

This is not recommended. Any trace amounts of water in the DMSO can promote spontaneous hydrolysis over time. Even if using anhydrous DMSO, DMSO is hygroscopic; it readily absorbs moisture from the atmosphere over time. A better alternative is to dissolve the reactive dye in a volatile solvent, make smaller aliquots and then evaporate off the solvent using a vacuum pump. The smaller aliquots of solid reactive dye should then be stored frozen, desiccated and protected from light. Contact Technical Support by sending an email to techsupport@thermofisher.com for the recommended volatile solvent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (7)

인용 및 참조 문헌

Abstract

Periostin and CCN2 Scaffolds Promote the Wound Healing Response in the Skin of Diabetic Mice.

Authors:Elliott CG, Wang J, Walker JT, Michelsons S, Dunmore-Buyze J, Drangova M, Leask A, Hamilton DW

Journal:Tissue Eng Part A

PubMed ID:30572781

'Nonhealing skin wounds remain a significant burden on health care systems, with diabetic patients 20 times as likely to undergo a lower extremity amputation due to impaired healing. Novel treatments that suppress the proinflammatory signature and induce the proliferative and remodeling phases are needed clinically. We demonstrate that the addition ... More

Massive and parallel expression profiling using microarrayed single-cell sequencing.

Authors:Vickovic S, Ståhl PL, Salmén F, Giatrellis S, Westholm JO, Mollbrink A, Navarro JF, Custodio J, Bienko M, Sutton LA, Rosenquist R, Frisén J, Lundeberg J

Journal:Nat Commun

PubMed ID:27739429

'Single-cell transcriptome analysis overcomes problems inherently associated with averaging gene expression measurements in bulk analysis. However, single-cell analysis is currently challenging in terms of cost, throughput and robustness. Here, we present a method enabling massive microarray-based barcoding of expression patterns in single cells, termed MASC-seq. This technology enables both imaging ... More

A two-hybrid antibody micropattern assay reveals specific

Authors:Dirscherl C, Hein Z, Ramnarayan VR, Jacob-Dolan C, Springer S

Journal:Elife

PubMed ID:30180933

We demonstrate a two-hybrid assay based on antibody micropatterns to study protein-protein interactions at the cell surface of major histocompatibility complex class I (MHC I) proteins. Anti-tag and conformation-specific antibodies are used for individual capture of specific forms of MHC I proteins that allow for location- and conformation-specific analysis by ... More

N-Linked Glycosylation Regulates CD22 Organization and Function.

Authors:Wasim L, Buhari FHM, Yoganathan M, Sicard T, Ereño-Orbea J, Julien JP, Treanor B

Journal:Front Immunol

PubMed ID:31019513

The organization and clustering of cell surface proteins plays a critical role in controlling receptor signaling; however, the biophysical mechanisms regulating these parameters are not well understood. Elucidating these mechanisms is highly significant to our understanding of immune function in health and disease, given the importance of B cell receptor ... More

Misfolded GPI-anchored proteins are escorted through the secretory pathway by ER-derived factors.

Authors:Zavodszky E, Hegde RS

Journal:Elife

PubMed ID:31094677

We have used misfolded prion protein (PrP*) as a model to investigate how mammalian cells recognize and degrade misfolded GPI-anchored proteins. While most misfolded membrane proteins are degraded by proteasomes, misfolded GPI-anchored proteins are primarily degraded in lysosomes. Quantitative flow cytometry analysis showed that at least 85% of PrP* molecules ... More

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