Alexa Fluor™ 594 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 594 NHS Ester (Succinimidyl Ester)
인용 및 참조 문헌 (2)
Invitrogen™

Alexa Fluor™ 594 NHS Ester (Succinimidyl Ester)

아민 반응성 isomeric 혼합물인 Alexa Fluor 594 carboxylic acid로 만들어지는 conjugate succinimidyl ester는 녹색 형광 conjugate와 스펙트럼 중복이 거의 없으며자세히 알아보기
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카탈로그 번호수량
A375723 x 100 μg
A200041 mg
A201045 mg
A3756525 mg
카탈로그 번호 A37572
제품 가격(KRW)
339,000
線上優惠
Ends: 31-Mar-2026
398,000
할인액 59,000 (15%)
Each
카트에 추가하기
수량:
3 x 100 μg
제품 가격(KRW)
339,000
線上優惠
Ends: 31-Mar-2026
398,000
할인액 59,000 (15%)
Each
카트에 추가하기
아민 반응성 isomeric 혼합물인 Alexa Fluor 594 carboxylic acid로 만들어지는 conjugate succinimidyl ester는 녹색 형광 conjugate와 스펙트럼 중복이 거의 없으며 Ar-Kr 레이저의 568 nm, 오렌지색 He-Ne 레이저의 594 nm까지 효율적으로 여기됩니다. Texas Red dye와 분광학적으로 유사한 Alexa Fluor 594 dye와 그 conjugate는 밝은 형광을 발하며 광안정성이 높습니다. 5 mg unit 크기로도 구입할 수 있습니다(카탈로그 번호: A-20104).
For Research Use Only. Not for use in diagnostic procedures.
사양
방출617 nm
여기590 nm
제품 유형Dye
수량3 x 100 μg
배송 조건Room Temperature
제품라인Alexa Fluor
Unit SizeEach
구성 및 보관
3 tubes
Store dessicated at -20°C, upon reciept

자주 묻는 질문(FAQ)

I am labeling a protein with Alexa Fluor 488 SDP ester. The manual recommends using a sodium bicarbonate buffer at pH 8.3. Can I use a different buffer instead?

Yes. The important thing is to use a buffered solution with a pH between 8.0 and 8.5. Do not use Tris buffer, which has amine groups. Most other buffers will work fine in that pH range. This is also true for other amine-reactive dyes, such as succinimidyl (NHS) esters or TFP esters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am not going to use all of my Alexa Fluor succinimidyl ester reactive dye. Can I just make it up in DMSO and store aliquots at -20 degrees C?

This is not recommended. Any trace amounts of water in the DMSO can promote spontaneous hydrolysis over time. Even if using anhydrous DMSO, DMSO is hygroscopic; it readily absorbs moisture from the atmosphere over time. A better alternative is to dissolve the reactive dye in a volatile solvent, make smaller aliquots and then evaporate off the solvent using a vacuum pump. The smaller aliquots of solid reactive dye should then be stored frozen, desiccated and protected from light. Contact Technical Support by sending an email to techsupport@thermofisher.com for the recommended volatile solvent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (2)

인용 및 참조 문헌
Abstract
A compact STED microscope providing 3D nanoscale resolution.
Authors:Wildanger D, Medda R, Kastrup L, Hell SW,
Journal:J Microsc
PubMed ID:19772534
'The advent of supercontinuum laser sources has enabled the implementation of compact and tunable stimulated emission depletion fluorescence microscopes for imaging far below the diffraction barrier. Here we report on an enhanced version of this approach displaying an all-physics based resolution down to (19 +/- 3) nm in the focal ... More
Quencher-free multiplexed monitoring of DNA reaction circuits.
Authors:Padirac A, Fujii T, Rondelez Y,
Journal:Nucleic Acids Res
PubMed ID:22753028
We present a simple yet efficient technique to monitor the dynamics of DNA-based reaction circuits. This technique relies on the labeling of DNA oligonucleotides with a single fluorescent modification. In this quencher-free setup, the signal is modulated by the interaction of the 3'-terminus fluorophore with the nucleobases themselves. Depending on ... More