Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester)
Invitrogen™

Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester)

무이성질체, 아민 반응성인 Alexa Fluor 647 carboxylic acid, succinimidyl ester dye와 conjugates는 Cy5 dye와 분광학적으로 동일하여 여기/방출 최대값이 ∼650/668 nm입니다.자세히 알아보기
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카탈로그 번호수량
A375733 x 100 μg
A200061 mg
A201065 mg
A3756625 mg
카탈로그 번호 A37573
제품 가격(KRW)
340,000
Online offer
Ends: 31-Mar-2026
400,000
할인액 60,000 (15%)
Each
카트에 추가하기
수량:
3 x 100 μg
제품 가격(KRW)
340,000
Online offer
Ends: 31-Mar-2026
400,000
할인액 60,000 (15%)
Each
카트에 추가하기
무이성질체, 아민 반응성인 Alexa Fluor 647 carboxylic acid, succinimidyl ester dye와 conjugates는 Cy5 dye와 분광학적으로 동일하여 여기/방출 최대값이 ∼650/668 nm입니다. 하지만 Alexa Fluor 647 conjugate는 Cy5 dye로 준비된 것보다 훨씬 밝습니다. A 5 mg 단위(A-20106)도 구매 가능합니다. 장파장 Alexa Fluor dye의 형광은 인간 눈에는 보이지 않지만 대부분의 영상 시스템으로는 쉽게 검출이 가능합니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
방출672 nm
여기651 nm
제품 유형Dye
수량3 x 100 μg
배송 조건Room Temperature
제품라인Alexa Fluor
Unit SizeEach
구성 및 보관
3 tubes
Store dessicated at -20°C, upon reciept

자주 묻는 질문(FAQ)

I am labeling a protein with Alexa Fluor 488 SDP ester. The manual recommends using a sodium bicarbonate buffer at pH 8.3. Can I use a different buffer instead?

Yes. The important thing is to use a buffered solution with a pH between 8.0 and 8.5. Do not use Tris buffer, which has amine groups. Most other buffers will work fine in that pH range. This is also true for other amine-reactive dyes, such as succinimidyl (NHS) esters or TFP esters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am not going to use all of my Alexa Fluor succinimidyl ester reactive dye. Can I just make it up in DMSO and store aliquots at -20 degrees C?

This is not recommended. Any trace amounts of water in the DMSO can promote spontaneous hydrolysis over time. Even if using anhydrous DMSO, DMSO is hygroscopic; it readily absorbs moisture from the atmosphere over time. A better alternative is to dissolve the reactive dye in a volatile solvent, make smaller aliquots and then evaporate off the solvent using a vacuum pump. The smaller aliquots of solid reactive dye should then be stored frozen, desiccated and protected from light. Contact Technical Support by sending an email to techsupport@thermofisher.com for the recommended volatile solvent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (15)

인용 및 참조 문헌
Abstract
Ultrahigh-resolution imaging reveals formation of neuronal SNARE/Munc18 complexes in situ.
Authors:Pertsinidis A, Mukherjee K, Sharma M, Pang ZP, Park SR, Zhang Y, Brunger AT, Südhof TC, Chu S,
Journal:
PubMed ID:23821748
'Membrane fusion is mediated by complexes formed by SNAP-receptor (SNARE) and Secretory 1 (Sec1)/mammalian uncoordinated-18 (Munc18)-like (SM) proteins, but it is unclear when and how these complexes assemble. Here we describe an improved two-color fluorescence nanoscopy technique that can achieve effective resolutions of up to 7.5-nm full width at half ... More
COPI buds 60-nm lipid droplets from reconstituted water-phospholipid-triacylglyceride interfaces, suggesting a tension clamp function.
Authors:Thiam AR, Antonny B, Wang J, Delacotte J, Wilfling F, Walther TC, Beck R, Rothman JE, Pincet F,
Journal:
PubMed ID:23901109
'Intracellular trafficking between organelles is achieved by coat protein complexes, coat protomers, that bud vesicles from bilayer membranes. Lipid droplets are protected by a monolayer and thus seem unsuitable targets for coatomers. Unexpectedly, coat protein complex I (COPI) is required for lipid droplet targeting of some proteins, suggesting a possible ... More
Arf1/COPI machinery acts directly on lipid droplets and enables their connection to the ER for protein targeting.
Authors:Wilfling F, Thiam AR, Olarte MJ, Wang J, Beck R, Gould TJ, Allgeyer ES, Pincet F, Bewersdorf J, Farese RV, Walther TC,
Journal:
PubMed ID:24497546
Lipid droplets (LDs) are ubiquitous organelles that store neutral lipids, such as triacylglycerol (TG), as reservoirs of metabolic energy and membrane precursors. The Arf1/COPI protein machinery, known for its role in vesicle trafficking, regulates LD morphology, targeting of specific proteins to LDs and lipolysis through unclear mechanisms. Recent evidence shows ... More
Targeted therapy of spontaneous murine pancreatic tumors by polymeric micelles prolongs survival and prevents peritoneal metastasis.
Authors:Cabral H, Murakami M, Hojo H, Terada Y, Kano MR, Chung UI, Nishiyama N, Kataoka K,
Journal:
PubMed ID:23801758
Nanoscaled drug-loaded carriers are of particular interest for efficient tumor therapy as numerous studies have shown improved targeting and efficacy. Nevertheless, most of these studies have been performed against allograft and xenograft tumor models, which have altered microenvironment features affecting the accumulation and penetration of nanocarriers. Conversely, the evaluation of ... More
ATP-independent diffusion of double-stranded RNA binding proteins.
Authors:Koh HR, Kidwell MA, Ragunathan K, Doudna JA, Myong S,
Journal:Proc Natl Acad Sci U S A
PubMed ID:23251028
The proteins harboring double-stranded RNA binding domains (dsRBDs) play diverse functional roles such as RNA localization, splicing, editing, export, and translation, yet mechanistic basis and functional significance of dsRBDs remain unclear. To unravel this enigma, we investigated transactivation response RNA binding protein (TRBP) consisting of three dsRBDs, which functions in ... More