무이성질체, 아민 반응성인 Alexa Fluor 647 carboxylic acid, succinimidyl ester dye와 conjugates는 Cy5 dye와 분광학적으로 동일하여 여기/방출 최대값이 ∼650/668 nm입니다. 하지만 Alexa Fluor 647 conjugate는 Cy5 dye로 준비된 것보다 훨씬 밝습니다. A 5 mg 단위(A-20106)도 구매 가능합니다. 장파장 Alexa Fluor dye의 형광은 인간 눈에는 보이지 않지만 대부분의 영상 시스템으로는 쉽게 검출이 가능합니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
방출672 nm
여기651 nm
제품 유형Dye
수량3 x 100 μg
배송 조건Room Temperature
제품라인Alexa Fluor
Unit SizeEach
구성 및 보관
3 tubes Store dessicated at -20°C, upon reciept
자주 묻는 질문(FAQ)
I am labeling a protein with Alexa Fluor 488 SDP ester. The manual recommends using a sodium bicarbonate buffer at pH 8.3. Can I use a different buffer instead?
Yes. The important thing is to use a buffered solution with a pH between 8.0 and 8.5. Do not use Tris buffer, which has amine groups. Most other buffers will work fine in that pH range. This is also true for other amine-reactive dyes, such as succinimidyl (NHS) esters or TFP esters.
I am not going to use all of my Alexa Fluor succinimidyl ester reactive dye. Can I just make it up in DMSO and store aliquots at -20 degrees C?
This is not recommended. Any trace amounts of water in the DMSO can promote spontaneous hydrolysis over time. Even if using anhydrous DMSO, DMSO is hygroscopic; it readily absorbs moisture from the atmosphere over time. A better alternative is to dissolve the reactive dye in a volatile solvent, make smaller aliquots and then evaporate off the solvent using a vacuum pump. The smaller aliquots of solid reactive dye should then be stored frozen, desiccated and protected from light. Contact Technical Support by sending an email to techsupport@thermofisher.com for the recommended volatile solvent.
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