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Thermo Scientific™
Pro-Detect™ Rapid Antibody Isotyping Assay Kit, rat
The Thermo Scientific Pro-Detect Rapid Antibody Isotyping Assay Kit - Rat is a single-use, dipstick lateral flow kit for quick,자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| A38551 | 10 tests |
카탈로그 번호 A38551
제품 가격(KRW)
370,000
Online offer
Ends: 31-Dec-2025
435,000할인액 65,000 (15%)
Each
수량:
10 tests
제품 가격(KRW)
370,000
Online offer
Ends: 31-Dec-2025
435,000할인액 65,000 (15%)
Each
The Thermo Scientific Pro-Detect Rapid Antibody Isotyping Assay Kit - Rat is a single-use, dipstick lateral flow kit for quick, easy determination of rat monoclonal antibody class and subclass identity. This antibody isotyping assay uses membrane-based strips coated with dye-bound conjugated antibodies that provide a visual, color readout of the monoclonal antibody isotype within ten minutes. The kit determines rat IgG1, IgG2a, IgG2b , and IgG2c.
Features of the Pro-Detect Rapid Antibody Isotyping Assay Kit:
• Long shelf life—stable for at least one year at 4°C
• Simple workflow—dilute antibody sample using provided diluent and dip in the lateral flow strip
• Fast—within 5–10 minutes a band appears that indicates the antibody isotype
• Sensitive—detect antibodies in tissue culture media or ascites fluid samples down to low ng/mL concentrations
• Specific—assay does not cross-react with fetal bovine serum (FBS)
• Reliable—results are similar to standard ELISA-based isotyping assays
Each Pro-Detect Rapid Antibody Isotyping assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to a test tube or microplate well and dipping in a lateral flow strip. Gold conjugates embedded in the strip membrane form specific class- and subclass-soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated regions of the membrane. Results are displayed as a red band above the printed class or sub-class description, indicating the antibody isotype present.
Determining the subclass of a rat antibody is important when selecting the best purification method. IgG2a, IgG2b, and IgG2c can be purified with Protein A columns at a pH of 7–8. IgG1 isotypes bind best to Protein A at a slightly higher pH of 8–9.
The Pro-Detect Rapid Antibody Isotyping Assay Kit is compatible with both tissue culture supernatant and mouse ascites fluid, and is more sensitive than conventional latex bead-based dipstick assays and much faster than ELISA-based isotyping assays. The kit requires only 0.5 μL ascites fluid, 2–22 μL of cell culture supernatant or 1.5 ng of purified antibody.
Features of the Pro-Detect Rapid Antibody Isotyping Assay Kit:
• Long shelf life—stable for at least one year at 4°C
• Simple workflow—dilute antibody sample using provided diluent and dip in the lateral flow strip
• Fast—within 5–10 minutes a band appears that indicates the antibody isotype
• Sensitive—detect antibodies in tissue culture media or ascites fluid samples down to low ng/mL concentrations
• Specific—assay does not cross-react with fetal bovine serum (FBS)
• Reliable—results are similar to standard ELISA-based isotyping assays
Each Pro-Detect Rapid Antibody Isotyping assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to a test tube or microplate well and dipping in a lateral flow strip. Gold conjugates embedded in the strip membrane form specific class- and subclass-soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated regions of the membrane. Results are displayed as a red band above the printed class or sub-class description, indicating the antibody isotype present.
Determining the subclass of a rat antibody is important when selecting the best purification method. IgG2a, IgG2b, and IgG2c can be purified with Protein A columns at a pH of 7–8. IgG1 isotypes bind best to Protein A at a slightly higher pH of 8–9.
The Pro-Detect Rapid Antibody Isotyping Assay Kit is compatible with both tissue culture supernatant and mouse ascites fluid, and is more sensitive than conventional latex bead-based dipstick assays and much faster than ELISA-based isotyping assays. The kit requires only 0.5 μL ascites fluid, 2–22 μL of cell culture supernatant or 1.5 ng of purified antibody.
For Research Use Only. Not for use in diagnostic procedures.
사양
어세이Isotyping Assay
검출 가능한 분석물질IgG1, IgG2a, IgG2b; IgG2c
용도(애플리케이션)Lateral Flow Rapid Assay
제품라인Pro-Detect
제품 유형Assay
수량10 tests
배송 조건Wet Ice
특이성Antibody
충분10 tests
검출 방법Chromogenic
타겟 종Rat
Unit SizeEach
구성 및 보관
• 10 Rapid Antibody Isotyping Assay Strips 1
• 10 Rapid Antibody Isotyping Assay Strips 2
• Sample Diluent, 4 mL
Sufficient for 10 isotype determinations
Store at 2–8°C.
• 10 Rapid Antibody Isotyping Assay Strips 2
• Sample Diluent, 4 mL
Sufficient for 10 isotype determinations
Store at 2–8°C.