EquiPhi29™ DNA Polymerase
EquiPhi29™ DNA Polymerase
Thermo Scientific™

EquiPhi29™ DNA Polymerase

Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution. This enzyme자세히 알아보기
Have Questions?
보기 방식 변경buttonViewtableView
카탈로그 번호수량ProductName
A39390250 U
A393911000 U
A393925000 U
3 옵션
카탈로그 번호 A39390
제품 정가(KRW)/ 250 U
제품 정가148,000
온라인 행사119,000온라인 주문시에만 적용 (Supply Center 제외) .
(ends 01-Jan-2025)
공급가
-
카트에 추가하기
수량:
250 U
대량 주문 또는 맞춤형 요청
제품 정가(KRW)/ 250 U
온라인 행사119,000온라인 주문시에만 적용 (Supply Center 제외) .
(ends 01-Jan-2025)
카트에 추가하기
EquiPhi29™ DNA Polymerase
카탈로그 번호A39390
제품 정가(KRW)/ 250 U
온라인 행사119,000온라인 주문시에만 적용 (Supply Center 제외) .
(ends 01-Jan-2025)
-
카트에 추가하기

Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution. This enzyme is significantly improved over phi29 DNA polymerase in protein thermostability, reaction speed, product yield, and amplification bias, while retaining all the benefits of the wild-type enzyme.

EquiPhi29 DNA Polymerase possesses strong strand displacement activity and 3'→5' proofreading exonuclease activity that acts preferentially on single-stranded DNA or RNA.

Features of EquiPhi29 DNA Polymerase
• Highest processivity and strand displacement activity—more than 70 kb long DNA stretches can be synthesized
• Low amplification bias
• Extremely high yields of amplified DNA, even from small amounts of template
• Highly accurate DNA synthesis in a short time 

Applications
EquiPhi29 DNA Polymerase may be used in a wide variety of applications including:
• Unbiased whole genome amplification (WGA)
• Rolling circle amplification (RCA)
• Protein-primed DNA amplification
• Cell-free cloning of lethal DNA
In situ genotyping with padlock probes
• RNA-primed DNA amplification

Notes
1. The addition of pyrophosphatase to the reaction mixture with EquiPhi29 DNA Polymerase may further enhance DNA synthesis
2. Because of the enzyme's 3'→5' proofreading exonuclease, 3’-modified primers are highly recommended.
3. EquiPhi29 DNA Polymerase can amplify DNA in a wide range of temperatures (30–45°C), with an optimal reaction temperature of 42°C.

For Research Use Only. Not for use in diagnostic procedures.
사양
제품 유형Stand-alone enzyme
용도(애플리케이션)MDA-WGA, RCA
중합효소EquiPhi29 DNA Polymerase
수량250 U
최적 반응 온도42°C
배송 조건Dry Ice
농도10 U/μL
반응 시간2 hours
Unit Size250 U
구성 및 보관

• EquiPhi29 DNA Polymerase (25 μL at 10 U/μL)
• 10X EquiPhi29 DNA Polymerase Reaction Buffer (0.25 mL)
• 110 mM DTT (0.25 mL)

Store at –15 to –25°C.