MultiShot™ DH5α T1R Chemically Competent E. coli Cells, 96-well plate

Invitrogen MultiShot DH5α T1R Chemically Competent E. coli Cells are a well-known, versatile strain that is used in many everyday cloning applications.

Features of MultiShot DH5α T1R competent cells include:

• TOP10 strain ideal for high-efficiency cloning and plasmid propagation
• Microtiter plate format design convenient for automated high-throughput cloning
• mcrA mutation that supports efficient transfection of methylated DNA
• lacZΔM15 allele that permits rapid blue/white screening of transfectants
• endA1 mutation that helps reduce endonuclease activity and improve plasmid DNA yields
• recA1 mutation that helps reduce unwanted recombination

Invitrogen DH5α T1R competent cells are prepared by a proprietary modification of the Hanahan method. They are suitable for use in high efficiency transformations in applications where a low amount of DNA is used or to construct libraries using plasmid-derived vectors. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α cells increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

The Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors and can be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. DH5α cells are capable of being transformed efficiently with large plasmids and can also serve as a host for the M13mp cloning vectors if a lawn of DH5αF'IQ (or similar) is provided to allow plaque formation.

F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1