MultiShot™ TOP10 Chemically Competent E. coli Cells, 96-well plate

Invitrogen MultiShot TOP10 Chemically Competent E. coli Cells are derived from DH10B cells and are one of the most popular laboratory strains. Provided in a clear skirted 96-well microtiter plate to simplify high-throughput workflows and provide transformation efficiencies of >1 x 10⁸ cfu/μg control plasmid DNA, Multishot TOP10 cells are a versatile strain that can be used in multiple routine cloning applications.

Features of MultiShot TOP10 competent cells include:

• TOP10 strain ideal for high-efficiency cloning and plasmid propagation
• Microtiter plate format design convenient for automated high-throughput cloning
• mcrA mutation that supports efficient transfection of methylated DNA
• lacZΔM15 allele that permits rapid blue/white screening of transfectants
• endA1 mutation that helps reduce endonuclease activity and improve plasmid DNA yields
• recA1 mutation helps reduce unwanted recombination

MultiShot TOP10 competent cells in 96-well plates are designed to facilitate high-throughput transformation. This format allows all steps of the transformation protocol, up to plating, to take place in the same well, saving time and reducing the chance of contamination. After the addition of DNA, MultiShot TOP10 competent cells can be transformed by heat-shock at 42°C using a heat block or thermocycler in the same plate.

Genotype

F^-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) endA1 nupG