Hepatic Spheroid Kit

The Gibco Hepatic Spheroid Kit is the first off-the-shelf kit for generation of hepatic spheroids in your own lab.Maintain cells자세히 알아보기

카탈로그 번호	수량
A41390	1 Kit

카탈로그 번호 A41390

제품 가격(KRW)

1 Kit

The Gibco Hepatic Spheroid Kit is the first off-the-shelf kit for generation of hepatic spheroids in your own lab.

Maintain cells in culture longer—up to 21 days, >4X longer than cells last in monolayer culture
Design your own schedule—cells are ready for plating when you are
Interrogate pathways of interest—applicable for toxicity, metabolism, or disease modeling

What you get:

Spheroid-qualified hepatocytes
Hepatocyte Thaw Medium
Plating supplement (Cocktail A + Dexamethasone)
Maintenance supplement (Cocktail B + Dexamethasone)
Fetal bovine serum
Williams' E Medium, no phenol red
Nunclon Sphera 96-well U-bottom plates

Please note that components are shipped separately due to differences in storage temperatures.

Simplified protocol

Cryopreserved cells can be thawed and plated at your convenience, in as little sa 60 minutes. Try it on your own, or request a demo with an experienced scientist.

Stringent quality control

Our high standards help ensure only the best spheroid-forming lots become available, maximizing your chance of success. In addition, each lot is tested for:

Albumin production in monolayer and spheroid culture
Phase I enzyme induction
Bile duct formation (CFDA staining)
Proper morphology
Viability >75%
Attachment efficiency
Monolayer formation and integrity

Important! For best results, do not attempt hepatic spheroid formation in low attachment plates from other sources. Hepatocyte lots are qualified in Nunclon Sphera plates only and use of other plates can lead to variability in spheroid formation.

Flexible readouts

Analyze single spheroids, or pool several for detection in assays such as gene expression, immunostaining, viability, or CYP activity.

For Research Use Only. Not for use in diagnostic procedures.

공여자 소스Single Donor

제품 유형Hepatocytes

수량1 Kit

배송 조건Cells: Dry Shipper, Plates: Room Temperature, Media/Reagents: Dry Ice

셀 유형Hepatocytes

형태Cryopreserved

종Human

Unit SizeEach

구성 및 보관

• Spheroid-qualified hepatocytes (1 mL, liquid N₂ vapor phase only)
• Hepatocyte thaw medium (45 mL, 2°C to 8°C)
• Plating supplement (Cocktail A + Dexamethasone) (18 mL, 2°C to 8°C)
• Maintenance supplement (Cocktail B + Dexamethasone) (20 mL, 2°C to 8°C)
• Fetal bovine serum (2 x 25 mL, -5°C to -30°C)
• Williams' E Medium, no phenol red (2 x 500 mL, 2°C to 8°C. Protect from light.)
• 8 Nunclon Sphera 96-well U-bottom plates

자주 묻는 질문(FAQ)

Who should I contact if I am looking for hepatocytes with particular characteristics?

Our hepatocytes are prequalified and sold according to application, such as induction, short-term metabolism, and transporter uptake. If you require hepatocytes with particular P450 values or donor specifications, contact our Hepatic Biology Tech Support Team (hepaticproducts@thermofisher.com.com) or (866) 952-3559 to help with specific lot recommendations.

Who can I call if I have technical questions about hepatocyte cell culture?

Thermo Fisher Scientific has a dedicated team to answer questions regarding hepatocyte cell culture. You can send a question by email (hepaticproducts@thermofisher.com) or contact us by phone: US toll-free: (866) 952-3559

Are there guidelines for the procurement of human liver tissue?

Thermofisher Scientific works with a variety of human tissue sources, including Tissue and Organ Procurement Organizations, qualified Research Tissue Organizations and prominent academic and medical centers through collaborations that follow rigorous regulations, certifications and/or accreditations (“Source Facilities”). Tissues obtained through source facilities are consistent with the applicable legal and ethical practices of the United States.

Source Facilities are required by federal law to maintain accurate records and donor confidentiality as well as adhere to certain applicable laws governing tissue used for research globally. These include, but are not limited to:

- 45 CFR Part 46, subparts A, B, C, D where applicable (US federal guidance on the use of human subjects in research)
- Health Insurance Portability and Accountability Act (HIPAA) (addressing issues of confidentiality of personal information)
- National Organ Transplant Act (42 CFR Part 482)
- The Uniform Anatomical Gift Act (UAGA) of 1968, revised 1984

Where do you get your human liver tissue?

The human liver cells are derived from tissue obtained from accredited institutions. Consent is obtained by these institutions from the donor or the donor’s legal next of kin, for the use of the tissue and its derivatives for research purpose.

What ratio of 3D-spheroid hepatocytes to hepatic stellate cells should I use in 3D-co-culture?

We have used a ratio between 10:1 and 3:1 for 3D-spheroid hepatocytes to stellate cells for a successful 3D co-culture. We would recommend testing different ratios as the cells may vary from one type of model to another.

View all Hepatic Spheroid Kit FAQs