EasyPep™ MS Sample Prep Kits
EasyPep™ MS Sample Prep Kits
Thermo Scientific™

EasyPep™ MS Sample Prep Kits

Generate high quality proteomic samples for MS analysis in less than four hours using optimized EasyPep MS Sample Prep kits.
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카탈로그 번호설명
A45734Maxi Sample Prep Kit
A40006Mini MS Sample Prep Kit
A57864Micro MS Sample Prep Kit
A45733MS Sample Prep Kit
A45735Lysis Buffer
A57865Peptide Clean-up Plate
카탈로그 번호 A45734
제품 가격(KRW)
2,599,000
Online offer
Ends: 31-Mar-2026
3,057,000
할인액 458,000 (15%)
Each
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설명:
Maxi Sample Prep Kit
제품 가격(KRW)
2,599,000
Online offer
Ends: 31-Mar-2026
3,057,000
할인액 458,000 (15%)
Each
카트에 추가하기

EasyPep Mini MS Sample Prep Kits enable efficient and reproducible processing of cultured mammalian cells and tissues for proteomic MS analysis. The kit contains pre-formulated buffers, MS-grade enzyme mix, peptide clean-up columns, and an optimized protocol to generate MS-compatible peptide samples in less than three hours. Kits are optimized to process protein samples from 10–100 μg with a high yield of MS-ready peptides.

Complete—includes pre-formulated reagents for lysis through digestion, peptide clean-up columns, and an optimized protocol for up to 20 samples
Optimized—streamlined protocol and reagents minimize the number of steps and time time needed to process samples
Flexible—reagents and protocol have been verified using cells, plasma, and tissue samples for 10 to 100 μg samples
Time-saving—sample processing time reduced from more than one day to less than three hours
Compatible—final preparation is ready for direct MS analysis and other downstream applications, including TMT labeling

The sample preparation of peptides for MS analysis is complex, with numerous steps and varied protocols, resulting in sample variability in both quality and quantity. This kit has been designed to improve reproducibility while saving hands-on and processing time. The number of steps and processing time have been reduced through the addition of Universal Nuclease to reduce viscosity from nucleic acids (replacing sonication), a rapid 'one pot' reduction/alkylation solution for cysteine modification (carbamidomethylation, + 57.02), and a trypsin/Lys-C protease mix for more complete digestion. In addition, the kit includes Peptide Clean-up columns and buffers to prepare detergent-free peptide samples for MS analysis. The optimized reagents and protocol produce high-quality peptides that are compatible with TMT labeling and other downstream applications, or are ready for MS analysis.

For Research Use Only. Not for use in diagnostic procedures.
사양
설명Maxi Sample Prep Kit
최종 제품 유형Peptides
용도(장비)Mass Spectrometer
라벨 또는 염료Unlabeled
수량8 Reactions
배송 조건Wet Ice
워크플로우 단계Protein Digestion
검출 방법Mass Spectrometry
제품라인EasyPep
제품 유형Sample Preparation Kit
시작 물질Protein Samples
Unit SizeEach
구성 및 보관
  • Lysis Solution, 25 mL, store at 4°C
  • Universal Nuclease, 25 kU, store at -20°C
  • Reduction Solution, 7 mL, store at 4°C
  • Alkylation Solution, 7 mL, store at 4°C
  • Enzyme Reconstititution solution, 7 mL, store at 4°C
  • Pierce™ Trypsin/Lys-C Protease Mix, MS Grade, 8 x 100 μg, store at -20°C
  • Digestion Stop Solution, 7 mL, store at 4°C
  • Peptide Clean-Up Columns, 8 Ea., store at 4°C
  • Wash Solution A, 40 mL, store at 4°C
  • Wash Solution B, 3 x 27 mL, store at 4°C
  • Elution Solution, 2 x 20 mL, store at 4°C
  • Low Protein Binding Collection Tubes, 2 mL, 40 Ea., store at 4°C

자주 묻는 질문(FAQ)

Are the EasyPep MS Sample Prep Kits compatible with vacuum pumps/dry-down devices?

As per protocol, peptide samples need to be dried down prior to LC-MS analysis. This step can be performed using vacuum pumps/dry-down devices without any harm caused to the device by the components present in the elution buffer within the EasyPep MS Sample Prep Kits.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

After cell lysis, my mass spectrometry sample is very viscous and difficult to pipette. How do I reduce the sample viscosity?

High sample viscosity after lysis is due to release of DNA from the nucleus. Sonication or addition of a nuclease such as the Pierce Universal Nuclease (Cat. No. 88700, 88701, or 88702) can be used to degrade DNA and reduce sample viscosity.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I process yeast samples with EasyPep Mini MS Sample prep Kit?

Add 0.5 mm diameter glass beads to the sample pelleted in Easy Pep Lysis Solution and proceed as recommended in the Easy Pep sample prep protocol. Vortex vigorously for few minutes, spin at 16,000 x g for 5 mins, and extract the supernatant for protein assay and further downstream sample preparation.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I process bacterial samples with EasyPep MS Sample Prep kits?

Add 1-2 µL of 50 mg/mL Lysozyme Solution (Cat. No. 90082) to 200 µL of the EasyPep lysis buffer provided in the kit and proceed with the sample prep. Add 200 µL of the prepared lysis solution to 5 X 10E8 bacterial cells (E. coli cells) (OD600 equal to 1, corresponding to 1 X 10E8 CFUs). Lyse by pipetting the sample repeatedly and centrifuge at 16,000 x g for 5 mins. Alternatively, E. coli cells can be lysed in lysis buffer using bead beating or sonication instead of lysozyme. After lysis, dilute samples to 1 mg/mL using lysis buffer, proceed with the EasyPep protocol for reduction, alkylation, digestion, and clean up as described in the product manual.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Do you offer a mass spec sample prep method for low abundant proteins in samples?

High Select Top14 Abundant Protein Depletion Mini Spin columns (Cat. Nos. A36369, A36370) can be used to deplete top 14 abundant plasma/serum proteins. The depletion of highly abundant proteins from the sample enables the detection and identification of low abundant proteins. The depleted samples can be processed with EasyPep MS sample prep kit to generate digested plasma/serum samples. The clean digested samples can be processed by nanoLC-MS/MS analysis for discovery experiments or nanoLC-PRM/MS analysis for targeted experiments.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.