GeneArt™ Gibson Assembly HiFi Master Mix
GeneArt™ Gibson Assembly HiFi Master Mix
Invitrogen™

GeneArt™ Gibson Assembly HiFi Master Mix

GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments자세히 알아보기
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카탈로그 번호수량
A4662850 Reactions
A4662710 Reactions
A46629200 Reactions
카탈로그 번호 A46628
제품 정가(KRW)/ 50 reactions
제품 정가942,000
온라인 행사753,600온라인 주문시에만 적용 (Supply Center 제외) .
(ends 01-Jan-2025)
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수량:
50 Reactions
제품 정가(KRW)/ 50 reactions
온라인 행사753,600온라인 주문시에만 적용 (Supply Center 제외) .
(ends 01-Jan-2025)
카트에 추가하기
GeneArt™ Gibson Assembly HiFi Master Mix
카탈로그 번호A46628
제품 정가(KRW)/ 50 reactions
온라인 행사753,600온라인 주문시에만 적용 (Supply Center 제외) .
(ends 01-Jan-2025)
-
카트에 추가하기
GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility of this approach is suitable for small and large DNA constructs and includes both single and multiple inserts. The resulting products can be used for a variety of downstream applications including transformation, PCR and rolling circle amplification (RCA). The GeneArt Gibson Assembly HiFi Master Mix kit includes master mix, positive control, and water and accommodates the use of your own competent cells.

Features of the GeneArt Gibson Assembly HiFi Master Mix include:
Simple—seamlessly assemble and clone up to six DNA fragments in a single reaction
Efficient—high fidelity provides more correct clones than other methods
Flexible—design guidelines allow assembly into any vector of your choice
Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells
Trusted—over 4000 citations in the scientific literature highlighting great success

Seamless assembly of multiple fragments
GeneArt Gibson Assembly HiFi cloning is a simple, one-step process whereby up to six fragments are combined in a proprietary enzymatic mix in order to assemble DNA fragments with shared terminal end homology without leaving any extra sequences or scars behind (seamless). After a 15–60 minute incubation, a portion of the assembly reaction is then transformed into chemically competent or electrocompetent cells, yielding clones that are ready for analysis the next day. The required 20- to 40-base pair end homology is designed into the de novo fragment for synthesis or can be easily engineered by PCR amplification with custom DNA oligos. This special enzyme mix creates a seamless and covalently bound DNA construct providing high efficiency.

Robust method for maximum efficiency
Due to the covalently bound final product, the GeneArt Gibson Assembly HiFi method allows the utilization of chemically competent cells or electrocompetent cells for the highest transformation efficiency, improving the success of finding the right clone, particularly for more challenging constructs. There is no need for restriction enzymes, ligation, or recombination sites, and the method provides a perfectly seamless construct without unwanted extra bases.

Provides great versatility
The GeneArt Gibson Assembly HiFi method provides versatility, can streamline many techniques through the rapid combination, addition, deletion, or exchange of DNA segments, and eliminates the need to subclone, saving time and effort in the cloning workflow.

Convenient formats
GeneArt Gibson Assembly HiFi kits are available in multiple formats, including:
GeneArt Gibson Assembly HiFi Cloning Kit, chemically competent cells (with One Shot TOP10 chemically competent cells)
GeneArt Gibson Assembly HiFi Cloning Kit, electrocompetent cells (with ElectroMAX DH10B electrocompetent cells)
• GeneArt Gibson Assembly HiFi Master Mix kits (cloning kit without the cells) (this page)

For Research Use Only. Not for use in diagnostic procedures.
사양
클로닝 방법Seamless cloning
제어 유형Positive Control
용도(애플리케이션)Seamless Cloning & Genetic Assembly
반응 수50 Reactions
단편 수Up to 6 fragments
제품라인GENEART™
제품 유형HiFi Master Mix
수량50 Reactions
배송 조건Dry Ice
워크플로우 단계DNA Assembly
Unit Size50 reactions
구성 및 보관
Includes master mix, positive control, and water and accommodates the use of your own competent cells.