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Oncomine™ Comprehensive Assay Plus, manual library preparation

The Oncomine Comprehensive Assay Plus is a targeted, next-generation sequencing (NGS) assay that provides a comprehensive genomic profiling solution appropriate자세히 알아보기
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카탈로그 번호수량
A4672124 Samples
카탈로그 번호 A46721
제품 가격(KRW)
-
수량:
24 Samples
The Oncomine Comprehensive Assay Plus is a targeted, next-generation sequencing (NGS) assay that provides a comprehensive genomic profiling solution appropriate for formalin-fixed paraffin-embedded (FFPE) tissues. The assay addresses multiple biomarkers associated with targeted and immune checkpoint therapies, covering over 500 genes, including comprehensive targets that are relevant in cancer. This version of the assay is designed for manual library preparation, not for automated library prepation using the Ion Chef System.

Features of the Oncomine Comprehensive Assay Plus include:
• Enables analysis of variants across 500+ genes
• Detection of SNVs, CNVs, InDels, TMB, MSI, and gene fusions
• Robust performance from as little as 10 ng per pool of nucleic acid isolated from FFPE samples including fine needle biopsies
• Characterized with molecular standards and controls
• Content driven by the Oncomine Knowledgebase and experienced scientists helps assure coverage of key targets aligned to published evidence

The Oncomine Comprehensive Assay Plus provides the reagents for library construction and four pools—two DNA and two RNA—of multiplex PCR primers for preparation of amplicon libraries from FFPE tumor samples. Designed to help you go from hundreds down to a few relevant cancer drivers, this assay is part of a comprehensive workflow that enables NGS data analysis from as little as 10 ng of nucleic acid per pool. The assay is optimized for sequencing on the Ion GeneStudio S5 systems with the Ion 550 Chip to enable up to four samples and a no-template control (NTC) per run. When combined with Oncomine Reporter Software, you can create reports that align labels, guidelines, and clinical trials to your results so you can focus on key drivers of cancer in your research.

From sample to answer
The Oncomine Comprehensive Assay Plus is part of an NGS workflow that helps researchers filter variant results from hundreds to just a few key cancer driver variants. Enabled by Oncomine Informatics, this filtering, as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.

Learn more about Oncomine Comprehensive Assay Plus ›

For Research Use Only. Not for use in diagnostic procedures.
사양
용도(애플리케이션)Sequencing
용도(장비)Ion GeneStudio S5 Prime
라이브러리Targeted Sequencing Library
샘플 수24
제품라인Oncomine
제품 유형Comprehensive Assay Plus
수량24 Samples
샘플 종류DNA, RNA
배송 조건Approved for shipment on Wet or Dry Ice
워크플로우 단계Library Generation
Sequencing TypeGenome and DNA Sequencing
Unit Size24 samples
구성 및 보관
• Oncomine Comprehensive Plus DNA Panel, store at -10 to -30°C
• Oncomine Comprehensive Panel v3M RNA, store at -10 to -30°C
• Ion AmpliSeq Library Plus Kit, store at -10 to -30°C

자주 묻는 질문(FAQ)

How many samples from the Oncomine Comprehensive Assay Plus, manual library preparation can I run on the Ion 550 Chip?

We recommend sequencing up to 5 DNA or RNA samples (4 samples and a non-template control (NTC)) on a single Ion 550 Chip.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Oncomine Comprehensive Assay Plus, manual library preparation?

The Oncomine Comprehensive Assay Plus, manual library preparation (Cat. No. A46721) is designed to prepare barcoded sample libraries from DNA and RNA. The kit consists of Oncomine Comprehensive Assay Plus DNA (2-pool) (Part No. A45615), RNA Oncomine Comprehensive Assay v3M (2-pool) (Part No. A33637), and two kits of Ion AmpliSeq Library Kit Plus (Cat. No. 4488990). Sufficient reagents are provided to prepare libraries from 24 samples.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How much of each sample is needed for the Oncomine Comprehensive Assay Plus, manual library preparation?

10 ng of nucleic acid input per pool (20 ng of DNA and 20 ng of RNA) isolated from FFPE samples, including fine needle biopsies, is needed for the Oncomine Comprehensive Assay Plus, manual library preparation.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What types of sample is the Oncomine Comprehensive Assay Plus, manual library preparation compatible with?

The assay is optimized for use with formalin-fixed paraffin embedded (FFPE) samples.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the Oncomine Comprehensive Assay Plus, manual library preparation?

The Oncomine Comprehensive Assay Plus, manual library preparation (Cat. No. A46721) is a targeted, next-generation sequencing (NGS) assay that enables the detection of single nucleotide variants (SNVs), insertions and deletions (INDELs), copy number variations (CNVs), gene fusions, splice variants, tumor mutational burden (TMB), and microsatellite instability (MSI) across 500+ unique genes. This version of the assay is designed for manual library preparation, not for automated library preparation using the Ion Chef System.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.