TaqMan™ Microbe Detection Assay
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Applied Biosystems™

TaqMan™ Microbe Detection Assay

Applied Biosystems TaqMan Microbe Detection and TaqMan Antibiotic Resistance assays are used for quantitative real-time PCR detection of microbial species자세히 알아보기
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카탈로그 번호수량
A50137250 μL
카탈로그 번호 A50137
제품 가격(KRW)
373,000
Each
주문하기
수량:
250 μL
Applied Biosystems TaqMan Microbe Detection and TaqMan Antibiotic Resistance assays are used for quantitative real-time PCR detection of microbial species and antibiotic resistance genes. These assays consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5’ end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3’ end.

Features of TaqMan Microbe Detection and TaqMan Antibiotic Resistance assays include:
Easy to use—just add DNA and master mix and run the qPCR—no melt curves required
Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
Sensitive—TaqMan assays are ideal for measuring low-abundance microbial targets
Accurate—identify microbial targets with high accuracy of quantitation
Extensive content—over 300 pre-designed microbe detection assays and 100 pre-designed antibiotic resistance assays currently available
Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for closely related microbial species and subtypes
Fast delivery—assays shipped in as little as 1–2 days (see below)

Approximate ship time
• Inventoried: 1–2 days in North America*
• Made to order: 4–6 days in North America

All of our predesigned TaqMan gene expression assays are covered by the TaqMan Assays qPCR Guarantee.**

*Our most popular assays are stocked in North America for overnight delivery where possible. If an assay is out of stock, it may take an additional 3–5 business days for delivery.

**Subject to terms and conditions. For complete details, go to www.thermofisher.com/taqmanguarantee.

사양
3'프라이머 수정FAM
5'프라이머 수정MGB-NFQ
형식Tube
반응 수250 Reactions
제품라인TaqMan
수량250 μL
배송 조건Room temperature
농도20X
검출 방법Primer-probe
용도(애플리케이션)Pathogen Detection
Unit SizeEach
구성 및 보관
Store at -15°C to -25°C.

자주 묻는 질문(FAQ)

What are the concentrations of the primers and probes for TaqMan microbial assays?

Our predesigned and custom TaqMan microbial 20X assays have a primer concentration of 900 nM and a probe concentration of 250 nM in the final 1X concentration.

Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.

For TaqMan Microbe Detection and Antibiotic Resistance Assays, what do the different banners mean (Qualified, Recommended, Control)?

- A recommended assay is the assay with the best strain coverage based on in silico prediction. In other words, recommended assays have the highest number of predicted on-targets and lowest number of predicted off-targets.
- A qualified assay has gone through R&D testing within the context of our main microbe detection applications, such as urinary tract, respiratory tract, and vaginal microbiota detection.
- A control banner is used exclusively for common control assays used in microbe detection applications.

I would like to order a control for my TaqMan Microbe Detection and Antibiotic Resistance Assay. Do you offer one? Do you have options for a custom control?

We have several controls for our pathogen-related content. To find a list of all available amplification controls, simply search in the Microbe Detection Search Tool (https://www.thermofisher.com/microbe-detection/taqman/query/) and click on the “Controls” option. Please contact Technical Support if you are unsure whether a particular control is compatible with your assay of interest. We also have options for creating a custom control, containing your specific targets of interest. You can find more information here: https://www.thermofisher.com/us/en/home/clinical/clinical-genomics/pathogen-detection-solutions/real-time-pcr-panel-controls.html

What protocol do you recommend using for the TaqMan Microbe Detection and Antibiotic Resistance Assays?

When using the assays in a single-tube format, we recommend following this general protocol (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018536_TaqManMicrobialAssays_SingleTube_QR.pdf).

How do I find the TaqMan Microbe Detection and Antibiotic Resistance Assay I'm looking for and search for multiple targets?

There are several ways to search for TaqMan Microbe Detection and Antibiotic Resistance Assays. From the main Thermo Fisher Scientific website page (www.thermofisher.com), hover over the word “Popular” in the top navigation bar. From the menu that comes up, select “TaqMan Real-Time PCR Assays”. Under “The right TaqMan Assay is just a couple of clicks away”, from the TaqMan Assay dropdown, select “Microbe & ABR Detection”. In the search box, you can then enter your target organism and find your assay of interest.

You can also use these friendly URLs:
https://www.thermofisher.com/microbe-detection/taqman/query/
https://www.thermofisher.com/antibiotic-resistance/taqman/query/

To search for multiple targets, from the search results displayed above, click on the link in the upper right for “Search Multiple Targets”.