Attune™ CytPix™ No-Wash No-Lyse Filter Kit

The Attune CytPix No-Wash No-Lyse Filter Kit enables configuration of the Attune CytPix Flow Cytometer with an additional side scatter channel off of the violet laser. This allows no-wash no-lyse immunophenotyping of whole blood. The Attune CytPix cytometer is quickly and easily configured to collect violet side scatter by changing dichroic and emission blades.

Enables no-wash/no-lyse application
Immunophenotyping of whole blood using traditional flow cytometry is typically performed with lysis of the red blood cells (RBCs) followed by a wash step. These steps can result in significant cell loss and damage.

While a no-wash, no-lyse method for immunophenotyping of whole blood has been previously described, the method is of limited utility in traditional flow cytometry, which uses hydrodynamic forces to orient cells in the flow stream, due to the high concentration of events being run through the system. Moreover, significant sample dilution is required in no-wash, no-lyse methods to achieve low coincidence with red blood cells, necessitating extremely long acquisition times.

With the Attune CytPix Flow cytometer you can acquire data quickly, even from dilute samples, using either a fluorescent reagent as a trigger to eliminate red blood cells or a differential side scatter using both blue and violet scatter all in combination with bright field imaging to aid in sample analysis and quality monitoring. This kit enables the side scatter approach.