AccelerOme™ TMTpro™ 16plex MS Sample Preparation Kits
AccelerOme™ TMTpro™ 16plex MS Sample Preparation Kits
Thermo Scientific™

AccelerOme™ TMTpro™ 16plex MS Sample Preparation Kits

TMTpro™ 16plex MS Sample Preparation Kits provide efficient and robust mass spec sample prep with TMTpro peptide labeling for use with the AccelerOme Automated Sample Prep System.
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카탈로그 번호수량충분
A509501 x 16 TMTpro Reactions30–100 μg samples
A509492 x 16 TMTpro Reactions10–30 μg samples
A509512 x 16 TMTpro Reactions30–100 μg samples
카탈로그 번호 A50950
제품 가격(KRW)
3,687,000
Each
카트에 추가하기
수량:
1 x 16 TMTpro Reactions
충분:
30–100 μg samples
제품 가격(KRW)
3,687,000
Each
카트에 추가하기
The AccelerOme TMTpro 16plex MS Sample Preparation Kits integrate pre-formulated buffers, MS-grade trypsin/Lys-C enzyme mix, and peptide clean-up cartridges with TMTpro 16plex label reagents for reproducible sample preparation of TMT-labeled peptides. The TMTpro 16plex label reagents have been optimized for use with samples from 10 to 100 μg samples to achieve greater than 95% labeling efficiency.

The Thermo Scientific Accelerome TMTpro 16plex MS Sample Preparation Kits provide a complete solution for efficient and robust proteomics mass spectrometry (MS) sample preparation, including TMT peptide labeling, for use with the AccelerOme Automated Sample Prep System.

TMTpro 16plex MS Sample Preparation Kits features:
Flexible—sample prep and TMT-labeling reagents verified on system using cells, plasma, proteins, and tissue samples for 10 to 100 μg of sample input (kit dependent)
Multiplex—enables concurrent MS analysis of up to 16 samples
Optimized — EasyPep kit chemistry combined with TMTpro 16plex labeling reagents specifically designed for use with the AccelerOme Automated Sample Prep System
Convenient—complete set of ready-to-use proteomic sample prep, clean-up, and TMT-labeling reagents for easy loading onto the instrument
Robust —reagents combined with AccelerOme ecosystem provide reproducible, high-quality TMT-labeled peptides with greater than 95% labeling efficiency

Kit components are single-use and conveniently provided in labeled trays for direct placement on the AccelerOme system. This kit provides a complete reagent solution for automated sample preparation of TMTpro 16plex labeled samples to produce high quality, MS-ready peptides.

The AccelerOme reagent portfolio is an integral part of the AccelerOme Automated system solution to provide robust proteomic mass spec sample preparation. The instrument hardware is built on a proven liquid handling format for reproducible sample processing and optimized on-line μSPE sample clean-up for excellent peptide recovery. TMT label reconstitution and sample labeling and pooling have been incorporated before sample clean-up. Peptide quantitation post-clean-up is achieved through accurate UV spectrophotometric analysis to determine peptide concentrations prior to LC-MS analysis. The complete system (kits + reagents + software) was designed to provide high quality peptides while improving productivity with an easy-to-use automated system. When combined with the industry-leading, high-resolution Orbitrap instruments and software, TMTpro 16plex reagents provide integrated total solutions for quantitative protein expression analysis.

These products are subject to a limited use label license.
 

사양
최종 제품 유형TMTpro-labeled peptide sample preparation
키트 구성품Sample prep module, TMT module
수량1 x 16 TMTpro Reactions
충분30–100 μg samples
워크플로우 단계Automated mass spec sample prep
검출 방법UV Absorption
형식Kit
제품라인Thermo Scientific
제품 유형Reagent Kit
시작 물질Protein Samples
Unit SizeEach

자주 묻는 질문(FAQ)

Can I use the AccelerOme TMT11plex MS Sample Preparation Kits and the AccelerOme TMTpro 16plex MS Sample Preparation Kits for samples with protein amounts lower than 10 µg?

Unfortunately, the AccelerOme TMT11plex MS Sample Preparation Kits and the AccelerOme TMTpro 16plex MS Sample Preparation Kits have not been validated for use with sample lysates containing lower than 10 µg of initial protein.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

With the AccelerOme TMT11plex MS Sample Preparation Kits and the AccelerOme TMTpro 16plex MS Sample Preparation Kits, can I use any other lysis buffer instead of the one in the kit?

We do not recommend using a different lysis buffer other than the one included in the kit. On the one hand, variation in the lysis buffer might affect the subsequent sample prep steps. On the other hand, volume of the different buffers included in the kit has been optimized for the correct performance of the Accelerome platform.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

When using the AccelerOme TMT11plex MS Sample Preparation Kits and the AccelerOme TMTpro 16plex MS Sample Preparation Kits with the AccelerOme platform, can I stop the process at any time?

We do not recommend stopping the automatized process as the AccelerOme platform is programmed to perform the complete sample preparation in a single run while keeping sample stability and reproducibility as its highest.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

When using the AccelerOme TMT11plex MS Sample Preparation Kits and the AccelerOme TMTpro 16plex MS Sample Preparation Kits, should I add a protease inhibitor cocktail to the lysis buffer? How about phosphatase inhibitors?

We do not recommend using protease inhibitor cocktails containing EDTA with the AccelerOme TMT11plex MS Sample Preparation Kits and the AccelerOme TMTpro 16plex MS Sample Preparation Kits as they will inhibit both Universal nuclease and protease Trypsin/Lys-C activities. Following cell lysis, reduction and alkylation, the Trypsin/Lys-C proteases mix is added for complete digestion. Therefore, if protease inhibitors containing EDTA are added, there will be incomplete proteolysis. On the other hand, we do recommend using a phosphatase inhibitor cocktail (e.g., Halt Phosphatase Inhibitor Cocktail) if a subsequent phosphopeptide enrichment analysis will be performed.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can I run the samples obtained from the AccelerOme TMT11plex MS Sample Preparation Kits and the AccelerOme TMTpro 16plex MS Sample Preparation Kits in any other mass spectrometer besides Orbitrap Mass Spectrometers?

We recommend using Orbitrap Mass Spectrometers as the analysis of TMT reagents requires a system that is able to perform high-resolution MS/MS fragmentation. Orbitrap Mass Spectrometers include a specific TMT MS2 workflow which enables unique acquisition management resulting in confident results without compromising coverage.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.