POROS™ Caprylate RoboColumn™ Pre-packed columns

These RoboColumns are pre-packed with POROS Caprylate Mixed-Mode Chromatography Resin to remove moderate to high levels of aggregates during downstream processing of therapeutic antibodies in flow-through mode. As a mixed-mode, weak cation-exchange resin, POROS Caprylate resin effectively removes other impurities, such as host cell proteins and a wide range of high molecular weight species. RoboColumns are small chromatography columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms.

Features of POROS Caprylate Mixed-Mode resin include:

• Selective capture of aggregates and hydrophobic high-molecular-weight impurities
• Flow-through design that helps save buffer and material costs
• Excellent scalability and high productivity
• Wide operational range
• Non-animal-derived

POROS Caprylate Mixed-Mode resin (Cat. No. A51051) operates in flow-through mode, which helps reduce costs, especially when working with more engineered monoclonal antibody derivatives. The unique selectivity of the caprylate ligand contributes to a high yield of the targeted monomer while using established cation exchange resin buffer systems. POROS Caprylate Mixed-Mode resin is manufactured using a synthetic manufacturing process that is free of animal components.

Main characteristics

• Matrix: cross-linked poly(styrene-divinylbenzene) POROS beads
• Average particle size: 50 μm
• Ligand: octanoic acid
• Binding capacity: >20 g lysozyme/L resin
• Typical buffers: typical cation exchange buffers, pH 4.5–7.0, conductivity 10–30 mS/cm
• Clean-in-place: 0.5 M NaOH
• Formulation buffer: 0.1 M NaOH
• Mechanical resistance: 100 bar (10 MPa, 1450 psi)

Other POROS chromatography resins are also available in this column format. All POROS high performance chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

RoboColumn™ is a registered trademark of ATOLL GmbH.