eBioscience™ Mouse Intracellular (nuclear/transcription factor) Protein Flow Cytometry Workflow Kit

The eBioscience Mouse Intracellular (nuclear/transcription factor) Protein Flow Cytometry Workflow Kit contains all the buffers, blockers, and beads to complete a standard flow cytometry experiment for staining intracellular nuclear and transcription factor targets in mouse cell suspensions.

See protocol ›

This kit contains:
• UltraComp eBeads Plus Compensation Beads (Cat. No. 01-3333-41)
• CD16/CD32 Monoclonal Antibody (93), eBioscience (14-0161-81)
• CellBlox Blocking Buffer (B001T06F01)
• eBioscience Foxp3/Transcription Factor Staining Buffer Set (00-5523-00)
• eBioscience Flow Cytometry Staining Buffer (00-4222-57)

When combined with the antibodies specific to your experiment, the reagents within this kit have been optimized to work together to detect cell surface targets by flow cytometry and broadly include:
• Effective compensation controls
• Fc receptor blocking
• Non-antibody, non-protein-based blocking solution for reducing background staining from fluorophores
• Antibody binding buffer that minimizes non-specific binding of antibodies
• Staining buffer optimized for transcription factors and nuclear proteins