CTS™ Detachable Dynabeads™ CD4
The Gibco™ CTS™ Detachable Dynabeads™ CD4 (SKU A56994) must be used with the CTS™ Detachable Dynabeads™ Release Buffer (SKU A5588301, A5588302, or A5588303) in order to trigger the active release mechanism that actively detaches magnetic beads from target cells.
CTS™ Detachable Dynabeads™ CD4
Gibco™

CTS™ Detachable Dynabeads™ CD4

CTS Detachable Dynabeads CD4 for positive isolation of CD4+ T cells and active release of Dynabeads post-isolation.
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카탈로그 번호수량
A5699415 mL vial
카탈로그 번호 A56994
제품 가격(KRW)
3,438,000
Each
카트에 추가하기
수량:
15 mL vial
제품 가격(KRW)
3,438,000
Each
카트에 추가하기
Gibco CTS Detachable Dynabeads CD4 are intended for ex vivo isolation of CD4+ T cell subsets. Combined with CTS Detachable Dynabeads Release Buffer (Cat. No. A5588301, A5588302, or A5588303), it is a first-of-its-kind cGMP cell selection technology with an active release mechanism for process development, clinical trial, and commercial manufacturing use. This product is also commonly used with CTS Detachable Dynabeads CD8 (Cat. No. A56995).

Features of CTS Detachable Dynabeads CD4 include:

  • Empower users with process flexibility by allowing active release of beads from target cells at any time
  • Optimize target cell purity and yield thanks to the active release mechanism that gives users more control
  • Provide an automation-friendly solution by working directly with the CTS DynaCellect Magnetic Separation System
  • Enable consistent performance in light of biological variability within the starting material
  • Suitable for both autologous and allogeneic cell therapy manufacturing processes

These Detachable Dynabeads are intended for use with CTS Detachable Dynabeads Release Buffer, which has an active ingredient, biotin, that competitively displaces a biotin derivative on the surface of the beads. This proprietary mechanism allows users to achieve active release of the beads from target cells at any time within their process.

Based on a recommended 4-to-1 bead-to-target-cell ratio (not total nucleated cell number), one 15 mL vial can theoretically isolate up to 1.5 x 109 CD4+ T cells.

CTS Detachable Dynabeads also include a Drug Master File (DMF) to support cross-referencing in regulatory submissions prior to clinical trial and commercial manufacturing use.

For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals.
사양
농도4 x 108 beads/mL
용도(장비)CTS™ DynaCellect™ Magnetic Separation System
제품라인CTS Detachable Dynabeads
순도 또는 품질 등급GMP-grade
수량15 mL vial
배송 조건Ships at 2°C to 8°C with wet (not frozen) gel packs. Vial must remain upright to keep beads in suspension.
부피(미터법)15 mL
색상Brown
직경(미터법)4.5 μm
물질Polystyrene
제품 유형Cell Isolation Reagent
Unit SizeEach
구성 및 보관
CTS™ Detachable Dynabeads™ CD4, 15 mL.

Store at 2°C to 8°C with vial upright to keep beads in suspension.

자주 묻는 질문(FAQ)

What is the definition of superparamagnetic, and what does this mean for my cell isolation application with Dynabeads magnetic beads?

Superparamagnetic means that the Dynabeads magnetic beads exhibit magnetic properties when placed within a magnetic field, but have no residual magnetism when removed from the magnetic field.

This means that your targeted cells, proteins, or nucleic acids are only subjected to magnetic forces during the time the beads are on the magnet. The beads do not aggregate, but remain evenly dispersed in suspension.

Find additional tips, troubleshooting help, and resources within our Dynabeads Cell Isolation and Expansion Support Center.

What is CTS?

The Gibco Cell Therapy Systems (CTS) portfolio of cell and gene therapy products are GMP manufactured, safety tested, and backed by regulatory documentation to support your transition from discovery through clinical and commercial manufacturing. Through our CTS solutions, we are committed to helping customers streamline therapeutic development, minimize risk, and ease the burden on their quality systems. Learn more here.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the shipping and storage and conditions for CTS Detachable Dynabeads CD4 (Cat. No. A56994)?

CTS Detachable DynabeadsCD3/CD28 (Cat. No. A56996) is shipped at 2-8 degrees C. We recommend storing it at 2-8 degrees C, protected from light.

Find additional tips, troubleshooting help, and resources within our Dynabeads Cell Isolation and Expansion Support Center.

What are the antibodies on the surface of CTS Detachable Dynabeads CD4 and CTS Detachable Dynabeads CD8?

CTS Detachable Dynabeads CD4 and CTS Detachable Dynabeads CD8 are coated with camelid-derived, single-domain (VHH) antibody fragments that identify and bind CD4 or CD8 markers, respectively. These antibodies are produced in an Animal Origin Free (AOF) yeast-based system virtually eliminating any risk of viral contamination.

Find additional tips, troubleshooting help, and resources within our Dynabeads Cell Isolation and Expansion Support Center.

When using CTS Detachable Dynabeads CD4 or CTS Detachable Dynabeads CD8, what is the recommended ratio of Dynabeads to target T cells?

When using CTS Detachable Dynabeads CD4 or CTS Detachable Dynabeads CD8, we recommend a 4:1 ratio of Dynabeads to target T cells (CD4+ or CD8+ T cells, respectively). Please do not calculate it based on the number of total nucleated cells in your starting material.

Find additional tips, troubleshooting help, and resources within our Dynabeads Cell Isolation and Expansion Support Center.