Pierce™ Crossflow Diafiltration Reservoir, 500 mL

Pierce Crossflow Filtration Cassettes are not GMP grade.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

A pressure gauge is provided with every Pierce Crossflow Filtration Cassette. Pierce Crossflow Filtration Cassettes can be run without a pressure gauge; however, it is important to limit the flow rate to 200-300 mL/min to prevent tearing and potential collapse of the tubing. The flow rate can be determined by measuring the volume flow over 1 min. Alternatively, a pump speed of 300-350 rpm or a pressure of 1-1.5 bar will maintain a flow rate of 200-300 mL/min.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

High protein concentration and/or presence of particulate in the sample can slow down the rate of concentration/diafiltration. Adsorption of the sample to the membrane and lower temperature (4 degrees C vs room temperature) can also slow down concentration/diafiltration. Filtering your sample prior to processing with a crossflow filtration cassette will help speed up concentration/diafiltration.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Crossflow ultrafiltration and stirred cell devices both effectively concentrate biomolecules with similar sample recoveries; however, a crossflow filtration device concentrates sample much faster than a stirred cell device.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Crossflow filtration is much faster for concentration and diafiltration than dialysis and chromatography methods.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.