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Thermo Scientific™
EquiPhi29™ DNA Amplification Kit
The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which자세히 알아보기
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보기 방식 변경
| 카탈로그 번호 | 수량 |
|---|---|
| A65393 | 100 Reactions |
| A65394 | 500 Reactions |
카탈로그 번호 A65393
제품 가격(KRW)
532,000
Online offer
Ends: 31-Dec-2025
625,000할인액 93,000 (15%)
Each
수량:
100 Reactions
제품 가격(KRW)
532,000
Online offer
Ends: 31-Dec-2025
625,000할인액 93,000 (15%)
Each
The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).
Features of the EquiPhi29 DNA Amplification Kit include:
• Fast DNA amplification in less than two hours
• Sensitivity from 1 fg of input DNA
• Maximal yield of 17 µg amplified DNA
• Compatibility with different types of sample input material (e.g., purified DNA, liquid media culture, agar plate colonies)
• Compatibility with various downstream applications such as DNA sequencing (Sanger sequencing, next-generation sequencing), digestion with restriction enzymes, cell-free DNA enrichment, and cell-free protein expression
• All required components for DNA amplification in RCA or MDA-WGA reactions
The kit contains EquPhi29 DNA Polymerase, a proprietary phi29 DNA polymerase mutant that possesses strong strand displacement activity and enables high thermostability, reaction speed, and product yield and low amplification bias. Also included is an optimized reaction buffer, exo-resistant random primer, pyrophosphatase, and dNTPs. The addition of pyrophosphatase to the reaction mixture enhances DNA amplification and facilitates sample handling.
For Research Use Only. Not for use in diagnostic procedures.
사양
형식Kit
최적 반응 온도42°C
반응 시간Up to 2 hours
배송 조건Dry Ice
용도(애플리케이션)RCA, MDA-WGA
형태Liquid
중합효소EquiPhi29 DNA Polymerase
제품 유형DNA Amplification Kit
수량100 Reactions
연구 카테고리Nucleic Acid Analysis, In Vitro Transcription, Cell-free Protein Expression, In Situ Sequencing, SNP Detection
Unit SizeEach
구성 및 보관
• EquiPhi29 DNA Polymerase (0.1 mL at 10 U/μL)
• 10X EquiPhi29 DNA Polymerase Reaction Buffer (1 mL)
• 0.1 M DTT (0.25 mL)
• 500 μM Exo-Resistant Random Primer (0.2 mL)
• 10 mM dNTP Mix (0.2 mL)
• 0.1 U/μL Pyrophosphatase, inorganic (0.1 mL)
Store at -15 to -25°C.