Verso 1-step RT-qPCR Kit, SYBR Green, with separate ROX vial
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Thermo Scientific™

Verso 1-step RT-qPCR Kit, SYBR Green, with separate ROX vial

The Thermo Scientific Verso 1-step RT-qPCR Kit for SYBR Green chemistry offers a quick and simple way to detect mRNA.자세히 알아보기
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200 Reactions
제품 가격(KRW)
2,070,000
キャンペーン価格
Ends: 30-Jun-2026
2,299,000
할인액 229,000 (10%)
Each
카트에 추가하기
The Thermo Scientific Verso 1-step RT-qPCR Kit for SYBR Green chemistry offers a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. This version of the kit comes with a separate vial of ROX reference dye.

Features of the Verso 1-step RT-qPCR SYBR Green Kit:

• Eliminate the need for DNase treatments—RT Enhancer (optional) prevents gDNA carry-over
• RT and qPCR steps performed in single tube—convenience and reduced contamination risk
• Use of the entire RT reaction as template for qPCR—improved sensitivity
• Verso RT enzyme is highly sensitive with broad dynamic range—accurate detection of RNA from 1 pg-1 ug
• Verso RT enzyme has a wide working temperature range (42 to 57°C)—improved success with GC-rich and other difficult templates
• Contains an inert blue dye for ease of pipetting during reaction setup

An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal for primer sequence optimization and collecting comparative data in experiments that do not require subsequent cDNA storage. The approach minimizes the risk of contamination, and improvements in reproducibility may be observed as a result of reduced sample handling.

Verso 1-Step RT-qPCR Kits for SYBR Green chemistry are compatible with all major real-time thermal cyclers.

Applications

• Gene expression analysis
• RNAi validation
• Microarray validation
• Pathogen detection
• Genetic testing
• Disease research

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Verso 1-step RT-qPCR Kit, SYBR Green, with separate ROX vial
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(장비)BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
형식Tube
반응 수200 Reactions
제품라인Verso
제품 유형1-Step RT-qPCR Kit
샘플 종류RNA
충분200 Reactions
검출 방법SYBR
용도(애플리케이션)Gene Expression, Pathogen Detection
GC-Rich PCR PerformanceHigh
PCR 방법1-step RT-qPCR
Unit SizeEach
구성 및 보관
Contains:
•1 x 50 μL Verso Enzyme Mix
•1 x 250μL RT enhancer
•2 x 1.25 mL 2X 1-Step qPCR SYBR Mix
•1 x 25μL ROX, 1mM

Store at -20°C

자주 묻는 질문(FAQ)

What is the RT Enhancer that is included in all Verso kits?

The RT Enhancer is a unique enzyme that degrades contaminating genomic DNA at the start of the RT step. It is equally as effective as DNase I treatment but more convenient as it eliminates the need for a separate DNase I incubation prior to RT-PCR.

Do I need to treat my RNA with DNase I before running RT-qPCR with Verso cDNA Synthesis Kit?

No, you do not need to treat your RNA with DNase I before running RT-qPCR with the Verso cDNA Synthesis Kit. The RT enhancer included in the Verso kit degrades any contaminating double-stranded DNA during the reverse transcription process, eliminating the need for a separate DNase I treatment.