MEGAscript™ SP6 Transcription Kit
MEGAscript™ SP6 Transcription Kit
Invitrogen™

MEGAscript™ SP6 Transcription Kit

Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced자세히 알아보기
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카탈로그 번호제품 유형수량
AM1330SP6 In Vitro Transcription kit40 Reactions
카탈로그 번호 AM1330
제품 가격(KRW)
657,000
Online offer
Ends: 31-Mar-2026
730,000
할인액 73,000 (10%)
Each
카트에 추가하기
제품 유형:
SP6 In Vitro Transcription kit
수량:
40 Reactions
제품 가격(KRW)
657,000
Online offer
Ends: 31-Mar-2026
730,000
할인액 73,000 (10%)
Each
카트에 추가하기
Novel transcription reaction conditions and Ambion's patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions. Each Ambion™ kit includes sufficient reagents for 40 reactions.

• Exclusive, ultrahigh yield technology is fast: 2 hour reaction time
• Amplifies aRNA for gene array analysis and other applications
• Efficiently incorporates many modified nucleotides

A typical 20 μl MEGAscript™ reaction with 1 μg of the pTRI-Xef-1alpha control template will yield over 100 μg of transcript. The versatility of the MEGAscript™ kit allows for manipulation of the reactions to include specialized reagents such as modified nucleotides, cap analog, or additional polymerase.

Accessory Products:
MessageAmp™ aRNA Amplification Kits are powered by MEGAscript™ technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, Ambion recommends using the MEGAclear™ Kit (SKU#AM1908) and the MEGAclear™-96 Kit (SKU#AM1909).
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(애플리케이션)In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
반응 수40 Reactions
제품라인Ambion, MEGAscript
제품 유형SP6 In Vitro Transcription kit
프로모터SP6
수량40 Reactions
배송 조건Dry Ice
시작 물질PCR Products, Linearized DNA plasmid
수율100 μg
최종 제품 유형Synthetic mRNA, mRNA
형식Kit
샘플 종류mRNA, Synthetic mRNA
Unit SizeEach
구성 및 보관
SP6 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.

자주 묻는 질문(FAQ)

I am using the MEGAscript SP6 Transcription Kit for in vitro transcription. I ran my reaction product on a denaturing gel and got a smear. Can you offer some advice?

If the RNA appears degraded (e.g., smeared), remove residual RNase from the DNA template preparation before in vitro transcription. Do this by digesting the DNA prep with proteinase K (100-200 µg/mL) in the presence of 0.5% SDS for 30 min at 50°C, and follow this with phenol/chloroform extraction. The RNase Inhibitor that is present in the transcription reaction can only inactivate trace RNase contamination. Large amounts of RNase contamination will compromise the size and amount of transcription products.