mMESSAGE mMACHINE™ T7 Transcription Kit

Ambion™ mMESSAGE mMACHINE™ High Yield Capped RNA Transcription Kits는 template DNA 1 μg에서 15–35 μg의 7-methyl guanosine capped RNA를 일반자세히 알아보기

카탈로그 번호	수량
AM1344	25 Reactions

카탈로그 번호 AM1344

제품 가격(KRW)

863,000

Online offer

Ends: 31-Mar-2026

958,000

할인액 95,000 (10%)

카트에 추가하기

25 Reactions

제품 가격(KRW)

863,000

Online offer

Ends: 31-Mar-2026

958,000

할인액 95,000 (10%)

카트에 추가하기

Ambion™ mMESSAGE mMACHINE™ High Yield Capped RNA Transcription Kits는 template DNA 1 μg에서 15–35 μg의 7-methyl guanosine capped RNA를 일반 합성하는 데 Ambion의 특허받은 고수율 transcription 기술을 사용합니다. 각 kit에는 반응 25회에 충분한 시약이 들어있습니다. • capped RNA의 높은 수율- 반응 당 최대 39 μg RNA • 확실한 편의성; cap analog-nucleotide mix 제공 • 간단한 반응 형식 • microinjection, in vitro translation, 또는 transfection에 이상적인 transcript 합성이 이루어짐 20 μl 반응에서 20시간 배양으로 mMESSAGE mMACHINE™ High Yield Capped RNA Transcription은 최대 35 μg capped RNA가 확보됨. 이는 기존 in vitro transcription 반응 수율의 10-50배 수준입니다. cap analog: GTP 비율이 최적화되어 수율(15–35 μg)과 capped되는 transcript 비율(80%) 간의 균형을 최상으로 유지합니다. mMESSAGE mMACHINE™ kits에는 capped RNA에서 단백질과 비통합 nucleotide를 분리하는 데 효율적인 LiCl 침전액도 들어있어 translation 효율을 높입니다. mMESSAGE mMACHINE™ Kit는 이 kit 안에 포함된 polymerases와 사용할 때만 최적화됩니다. 그 외 다른 polymerase 사용 시 수율이 낮아질 수 있습니다. 각 mMESSAGE mMACHINE™ Kit 구성: 57 U cap analog, 비용 대비 최고의 가치.

For Research Use Only. Not for use in diagnostic procedures.

용도(애플리케이션)In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products

반응 수25 Reactions

제품라인Ambion, mMESSAGE mMACHINE

제품 유형T7 In Vitro Transcription kit

프로모터T7

수량25 Reactions

배송 조건Dry Ice

시작 물질PCR Products, Linearized DNA plasmid

수율20-30 μg

최종 제품 유형Synthetic mRNA, mRNA

형식Kit

샘플 종류mRNA, Synthetic mRNA

Unit SizeEach

구성 및 보관

T7 Enzyme Mix, 10X Reaction Buffer 2XNTP/CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.

자주 묻는 질문(FAQ)

I am using the mMESSAGE mMACHINE T3 Transcription Kit for in vitro transcription. I ran my reaction product on a denaturing gel and got a smear. Can you offer some advice?

If the RNA appears degraded (e.g. smeared), remove residual RNase from the DNA template preparation before in vitro transcription. Do this by digesting the DNA prep with proteinase K (100-200 µg/mL) in the presence of 0.5% SDS for30 min at 50°C, follow this with phenol/chloroform extraction. The RNase Inhibitor that is present in the transcription reaction, can only inactivate trace RNase contamination. Large amounts of RNase contamination will compromise the size and amount of transcription products.

What is the sequence for the pTri-Xef control template that comes with mMESSAGE mMACHINE T7 Transcription Kit (Cat. No. AM1344)?

The pTri-Xef control template that comes with mMESSAGE mMACHINE T7 Transcription Kit (Cat. No. AM1344) is a linearized TRIPLEscript DNA plasmid containing the 1.85 kb Xenopus elongation factor 1α gene under the transcriptional control of tandem SP6, T7, and T3 promoters (pTRI-Xef 1). While we cannot provide a full map of the linearized plasmid, the insert sequence with the three promotors is as follows:

ACGATTTAGGTGACACTATAGAATACACGGAATTAATACGACTCACTATAGGGAGACTCGAGAATTAACCCTCACTAAAGGGAGGTACCGCGGATGCATGAATTCCGCCGGTGAGTTCTAACTATCCACCGCCAAACATCTAATCCACAATGGGAAAGGAAAAGACTCACATCAACATCGTTGTCATTGGACACGTAGATTCTGGAAAGTCCACAACAACTGGACATCTGATCTACAAATGTGGTGGTATCGACAAGAGAACCATCGAAAAGTTCGAGAAGGAAGCTGCTGAGATGGGAAAAGGCTCCTTCAAGTATGCCTGGGTCTTGGACAAACTGAAGGCCGAGCGTGAACGTGGTATTACCATTGACATCTCCCTGTGGAAATTTGAGACCAGCAAATACTATGTCACTATCATTGATGCTCCTGGACACAGAGATTTCATCAAGAACATGATCACTGGTACTTCTCAGGCTGACTGTGCTGTCCTGATTGTTGCTGCTGGTGTTGGTGAATTTGAAGCTGGTATCTCAAAGAATGGACAAACCCGTGAGCATGCCCTCCTTGCCTACACTCTGGGTGTAAAACAGCTGATCGTTGGTATTAACAAGATGGATTCCACTGAACCCCCATACAGTCAGAAAAGATATGAGGAAATTGTAAAGGAAGTCAGCACATACATCAAGAAGATTGGTTACAACCCTGACACTGTTGCCTTTGTACCTATTTCTGGATGGAACGGTGACAACATGCTTGAGCCTAGCCCCAATATGCCATGGTTTAAGGGATGGAAAATCACCCGTAAAGAAGGATCTGGCAGCGGAACTACCCTGCTGGAAGCTCTTGACTGCATTCTGCCACCTAGTCGCCCAACTGATAAGCCTCTCCGTCTGCCTCTGCAGGATGTCTACAAAATTGGCGGTATTGGTACTGTACCAGTTGGTCGTGTGGAAACTGGTGTCATTAAACCAGGCATGGTGGTTACCTTTGCCCCTGTTAATGTAACAACTGAAGTGAAATCTGTTGAAATGCACCATGAAGCCCTTACTGAGGCCGTGCCCGGTGACAACGTCGGCTTTAACGTCAAGAACGTTTCCGTGAAGGACGTCCGTCGTGGCAACGTTGCTGGTGACAGCAAGATTGACCCACCAATGGAAGCTGGTTCTTTTACCGCACAGGTTATCATCCTGAATCACCCAGGCCAGATTGGTGCTGGATATGCCCCTGTGTTGGATTGCCACACTGCTCACATTGCTTGCAAGTTTGCTGAGCTAAAGGAGAAGATTGATCGCCGTTCTGGTAAGAAACTGGAAGACAATCCCAAGTTCCTGAAGTCTGGAGATGCTGCCATTGTTGACATGATCCCAGGAAAGCCAATGTGCGTGGAGAGCTTCTCAGACTACCCTCCTCTTGGTCGTTTTGCTGTCCGTGACATGAGGCAGACTGTTGCCCTAGGAGTCATCAAGGCAGTGGAAAAGAAGGCAGCAGGATCTGGCAAAGTCACAAAGTCTGCTCAGAAAGCAGCGAAAACCAAATGAATATTTCCAGAGTCTCTCACCTCAGTCATAACCAGTGGTGGAGGATTGGTCTCAGAACTCTTTCTATCAATTGGCCATCAGAGTTTAATAGTAAAAGACTGGTTAATGATTACAATGCATCGCAAGAGCTTCAGAAGGAAAAAAATGCTTGTGGACACATTTGTTTGTGGCAGTTTTAAGTTATTAGTGTTTTAAATCCAGTAATTTCTAAATGGAAGCAAATTGACCAAAATCTGTCACCAAATTTGAGACCATTAAAAAAAAGTTGAAAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGGAATTTCGCGGAATTCGAGCTCGCCCGGGGATCCTCTAGAGTCGACCTGCAGCCCA

SP6 (ATTTAGGTGACACTATAGA) 1.92 kb
T7 (TAATACGACTCACTATAGG) 1.89 kb
T3 (AATTAACCCTCACTAAAGG) 1.86 kb

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