mMESSAGE mMACHINE™ T3 Transcription Kit

mMESSAGE mMACHINE high yield capped RNA transcription kits use patented high-yield transcription technology for the routine synthesis of 15–35 μg of 7-methyl guanosine capped RNA from 1 μg of template DNA. Each kit includes sufficient reagents for 25 reactions.

• High yields of capped RNA—up to 35 μg of RNA per reaction
• Extra convenience—cap analog-nucleotide mix provided
• Simplified reaction format
• Synthesized transcripts ideal for microinjection, in vitro translation, or transfection
• 10–50 times yield obtained with conventional in vitro transcription reactions
• Ratio of cap analog to GTP has been optimized to allow compromise between yield (15–35 μg) and proportion of transcripts that are capped (80%)
• Contains a LiCl precipitation solution, efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation
• Optimized for use with polymerases included in kit
• Contains 57 U of cap analog