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<i>mir</i>Vana&trade; miRNA Isolation Kit, with phenol
인용 및 참조 문헌 (6)
Invitrogen™

mirVana™ miRNA Isolation Kit, with phenol

mirVana™ miRNA Isolation Kit는 효율적인 유리섬유필터(GFF) – 기반 방법을 사용해 조직과 세포에서 작은 RNA를 신속하게 분리합니다. 이 방법은 kilobase에서 10-mers까지자세히 알아보기
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카탈로그 번호수량
AM156040 Preps
카탈로그 번호 AM1560
제품 가격(KRW)
588,000
キャンペーン価格
Ends: 31-Mar-2026
653,000
할인액 65,000 (10%)
Each
수량:
40 Preps
제품 가격(KRW)
588,000
キャンペーン価格
Ends: 31-Mar-2026
653,000
할인액 65,000 (10%)
Each
mirVana™ miRNA Isolation Kit는 효율적인 유리섬유필터(GFF) – 기반 방법을 사용해 조직과 세포에서 작은 RNA를 신속하게 분리합니다. 이 방법은 kilobase에서 10-mers까지 범위의 total RNA를 분리합니다. 각 kit에는 total RNA (작은 RNA 포함) 40회 분리 또는 대형 및 소형 RNA fraction 20회 분리에 충분한 시약과 소모품이 들어있습니다.

• 효율적인 small RNA – 포함 total RNA 분리
• 200 nt 미만의 작은 RNA에 대한 성능이 강화되어 다음 단계 분석의 민감도가 향상됨
• 간단한 30분 절차
• miRNA, siRNA, shRNA, snRNA 분석에 이상적
• 실제로 모든 세포와 조직 유형에 이용가능함

작은 RNA 회수에 효율적임
현재 RNA 정제 방법은 유기 추출 후 알코올 침전을 하거나 GFF 또는 실리케이트 매트릭스에 핵산 분자를 흡수시킵니다. 아쉽게도 이전 방법은 시간이 많이 걸리고 낮은 RNA 회수율에 비효율적인 알코올 침전이 필요합니다. 두 번째 방법으로는 일반적으로 작은 RNA가 상당량 손실됩니다. mirVana™ miRNA Isolation Kit는 유기 추출 후 특수화된 결합 및 세척 조건에서 GFF 정제가 이루어집니다. 그래서 이 kit는 실제로 모든 세포와 조직 유형에서 모든 RNA, 즉 큰 mRNA와 리보솜 RNA를 10-mers까지 효율적으로 회수합니다. mirVana™ miRNA Isolation Kit를 사용하면 102 – 107 배양 세포 또는 0.5 – 250 mg 조직으로 이루어진 샘플에서 작은 RNA를 함유한 total RNA를 분리할 수 있습니다.

작은 RNA에 대해 강화
Although the mirVana™ miRNA Isolation Kit가 10 nt 이상의 RNA를 모두 효율적으로 정제하지만 작은 RNA species에서 확실히 강화 또는 제거된 RNA fraction을 분리하는 절차도 포함합니다. mirVana™ miRNA Isolation Kit에 포함된 이런 강화 절차를 통해 큰 RNA species에서 200nt 이하의 mRNA 분자를 효율적으로 정제할 수 있습니다. 이런 RNA 준비의 결과로 miRNAs, siRNAs, 및/또는 snRNAs에 대해 성능이 매우 강화됩니다. mirVana™ miRNA Isolation Kit 절차를 이용한 작은 RNA 강화로 동일한 분석을 총 RNA에사용했을 때보다 작은 RNA 검출에서 민감도를 높이고 배경을 줄입니다.

부속 제품:
Acid Phenol:Chloroform 같은 표준 키트에 들어있는 유기 화학물 배송 비용을 우려하시는 분들은 mirVana™ miRNA Isolation Kit without Phenol을 구매할 수 있습니다.

이 제품은 연구용으로만 사용가능합니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
용리량100 μL
최종 제품 유형Small RNA
용도(애플리케이션)qRT-PCR, microarray analysis, Northern blotting
고처리량 호환성Not High-throughput Compatible (Manual)
정제 시간30 min. (After sample homogenization)
수량40 Preps
배송 조건Room Temperature
시작 물질 양Bacteria: ≤107 cells
Cells: ≤107
Plant: ≤250 mg
Tissue: ≤250 mg
Virus: ≤107 cells
Yeast: ≤107 cells
Enzymatic Reactions: varies
수율Cells: ≤16 μg RNA per 50,000 cells
Tissue: ≤160 μg per 5 mg
Isolation TechnologySpin Column (Glass Fiber Filter), Organic Extraction
샘플 종류Bacteria, Cells, Plant, Tissue, Viruses, Yeast, Enzymatic Reactions
Unit SizeEach
구성 및 보관
• 30 mL miRNA Wash Solution I (room temperature)
• 50 mL Wash Solution 2/3 (room temperature)
• 80 Collection Tubes (room temperature)
• 40 Filter Cartridges (room temperature)
• 100 mL Lysis/Binding Buffer (4°C)
• 10 mL miRNA Homogenate Additive (4°C)
• 100 mL Acid-Phenol:Chloroform (4°C)
• 1.4 mL Gel Loading Buffer II (-20°C)
• 5 mL Elution Solution (-20°C, 4°C, or room temperature)

자주 묻는 질문(FAQ)

What kit would you recommend for isolation of miRNA from tissue, cells, or liquid samples?

For tissue or cells, we recommend the mirVana miRNA Isolation Kit (Cat. No. AM1560), while for liquid samples, we recommend the mirVana Paris RNA and Native Protein Purification Kit (Cat. No. AM1556). For high-throughput miRNA isolation we recommend using the MagMAX mirVana Total RNA Isolation kit (Cat. No. A27828)

What are different methods for cleaning up my miRNA sample?

- Precipitation of miRNA: Follow the instructions for the mirVANA miRNA Isolation Kit or the mirVANA PARIS RNA and Native Protein Purification Kit.
- Double phenol/guanidium (chaotropic reagent) extraction: Disrupt tissue with phenol/guanidine, then extract with phenol, centrifuge, remove the supernatant, and precipitate the RNA.
- Glass-fiber filter: Disrupt the tissue in a chaotropic agent, bind the RNA to a glass-fiber filter, then wash off unbound DNA/protein/salt/nucleotides and elute the RNA.

What type of sample can be used with TaqMan MicroRNA Assays?

One to ten nanograms of total RNA per 15 µL RT reaction is recommended for use with the TaqMan MicroRNA Assay. We have successfully detected miRNAs in as little as 25 pg of total RNA (~100 cells) in samples where the miRNA was highly abundant. The total RNA isolation procedure used must preserve small RNA species. We recommend the Invitrogen mirVana miRNA Isolation Kit (Cat. No. AM1560) or TRI reagent (Cat. No. AM9738).

When using the mirVana miRNA Isolation Kit, with phenol (Cat. No. AM1560), can I store homogenized tissue RNA lysate in the lysis buffer at -80 degrees C?

Yes, the RNA lysates can be stored at -80 degrees C for up to 2 months.

What kit do you recommend to capture small RNA that is &gt;17 nt in size?

We recommend using the mirVana miRNA Isolation Kit (Cat. No. AM1560, AM1561), both of which are designed to capture small RNAs, such as miRNAs, tRNAs, etc. Purification of RNAs as small as 20 nt has been obtained in-house using these kits. Note: Cat. No. AM1561 does not contain phenol, but is required for the procedure. Phenol can be purchased separately (Cat. No. AM9720).

인용 및 참조 문헌 (6)

인용 및 참조 문헌
Abstract
[Differential expression profile of microRNA between hyperplastic scar and normal skin].
Authors:Ning P, Liu DW, Mao YG, Peng Y, Lin ZW, Liu DM,
Journal:Zhonghua Yi Xue Za Zhi
PubMed ID:22781298
To explore the miRNA differential expression profiles of hyperplastic scar and normal skin so as to further elucidate the pathogenesis of hyperplastic scar and search for new therapeutic targets. The total RNA was extracted from 5 human hyperplastic scar and normal skin tissues by Trizol. The specimens were collected from ... More
Circulating microRNAs in serum: novel biomarkers for patients with bladder cancer?
Authors:Scheffer AR, Holdenrieder S, Kristiansen G, von Ruecker A, Müller SC, Ellinger J,
Journal:World J Urol
PubMed ID:23266581
'PURPOSE: Recent studies indicate that circulating microRNAs in serum/plasma are a novel class of non-invasive biomarkers with diagnostic and prognostic information. So far, circulating microRNAs have not been analyzed in patients with bladder cancer. METHODS: We collected serum from patients with non-muscle invasive bladder cancer (NMIBC), muscle invasive bladder cancer ... More
[Isolation, identification and analysis of the expression profile of miRNAs in Aedes albopictus].
Authors:Zheng PM, Wu JY, Gu JB, Tu ZJ, Chen XG,
Journal:Nan Fang Yi Ke Da Xue Xue Bao
PubMed ID:20423824
'To verify the miRNA in Aedes albopictus and characterize the expression profile of several miRNAs across all the life stages of Aedes albopictus. Based on the published miRNA sequences of Anopheles gambiae and Aedes aegypti, 6 DIG-labeled antisense probes were synthesized. The total RNAs from Aedes albopictus in 6 developmental ... More
MicroRNA expression profiles in the progression of prostate cancer-from high-grade prostate intraepithelial neoplasia to metastasis.
Authors:Leite KR, Tomiyama A, Reis ST, Sousa-Canavez JM, Sañudo A, Camara-Lopes LH, Srougi M,
Journal:Urol Oncol
PubMed ID:21880514
'Models of the multistep process related to cancer progression have been designed for many cancers including prostate. The aim of this study is to propose a new model including a possible role for recently described micro RNAs in prostate cancer (CaP) progression. Sixty-three patients underwent radical prostatectomy to treat localized ... More
A model for data analysis of microRNA expression in forensic body fluid identification.
Authors:Wang Z, Luo H, Pan X, Liao M, Hou Y,
Journal:Forensic Sci Int Genet
PubMed ID:21903498
MicroRNAs (miRNAs, 18-25 bases in length) are small, non-coding RNAs that regulate gene expression at the post-transcriptional level. MiRNA expression patterns, including presence and relative abundance of particular miRNA species, provide cell- and tissue-specific information that can be used for body fluid identification. Recently, two published studies reported that a ... More
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