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인용 및 참조 문헌 (1)
Invitrogen™
RNAqueous™-4PCR Total RNA Isolation Kit
The RNAqueous-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 to 75 mg) or from자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| AM1914 | 30 Preps |
카탈로그 번호 AM1914
제품 가격(KRW)
421,000
キャンペーン価格
Ends: 31-Dec-2025
467,000할인액 46,000 (10%)
Each
수량:
30 Preps
제품 가격(KRW)
421,000
キャンペーン価格
Ends: 31-Dec-2025
467,000할인액 46,000 (10%)
Each
The RNAqueous-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 to 75 mg) or from cells (∽100 cells to ∽1 x 107 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR.
- DNA-free RNA for RT-PCR
- Rapid, phenol-free total RNA isolation
- Novel reagent for rapid and safe removal of DNase and divalent cations
Using the RNAqueous-4PCR Kit
The RNAqueous-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with RNAlater™ Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. No need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.
Not high-throughput compatible (Manual)
Applications:
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues
For Research Use Only. Not for use in diagnostic procedures.
사양
용리량50–120 μL
최종 제품 유형Total RNA
용도(애플리케이션)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
고처리량 호환성Not High-throughput Compatible (Manual)
정제 시간10 min. (After sample homogenization)
수량30 Preps
배송 조건Box 1: Dry Ice, Box 2: Room Temperature
시작 물질 양Tissue: ≤75 mg, Cells: ≤107
수율≤10 μg per 1 mg tissue, ≤1 μg per 1 x 105 cells
Isolation TechnologySilica Spin Column
샘플 종류Tissue, Cells
Unit SizeEach
구성 및 보관
• 600 μL 10X DNase 1 Buffer; store at -20°C
• 45 μL DNase 1; -20°C
• 1 mL Formaldehye Load Dye; -20°C
• 100 μL Linear Acrylamide; -20°C
• 50 μL Positive Control Primers; -20°C
• 1 mL 5M Ammonium Acetate; -20°C
• 35 mL Water; 4°C
• 600 μL DNase Inactivation Reagent; 4°C
• 30 mL Lysis/Binding Solution; 4°C
• 25 mL Wash Solution #1; 4°C
• 35 mL Wash Solution 2/3 (concentrated); 4°C
• 3 Pestles; room temperature
• 30 Filter Cartridges; room temperature
• 60 Collection Tubes; room temperature
• 25 mL Elution Solution; room temperature
• 45 μL DNase 1; -20°C
• 1 mL Formaldehye Load Dye; -20°C
• 100 μL Linear Acrylamide; -20°C
• 50 μL Positive Control Primers; -20°C
• 1 mL 5M Ammonium Acetate; -20°C
• 35 mL Water; 4°C
• 600 μL DNase Inactivation Reagent; 4°C
• 30 mL Lysis/Binding Solution; 4°C
• 25 mL Wash Solution #1; 4°C
• 35 mL Wash Solution 2/3 (concentrated); 4°C
• 3 Pestles; room temperature
• 30 Filter Cartridges; room temperature
• 60 Collection Tubes; room temperature
• 25 mL Elution Solution; room temperature
자주 묻는 질문(FAQ)
What are the differences in the RNAqueous kits you offer?
인용 및 참조 문헌 (1)
인용 및 참조 문헌
Abstract
Methods for gene silencing with RNAi.
Journal:Methods Mol Biol
PubMed ID:17951785
This chapter describes a technique for synthesizing and transfecting double stranded RNA (dsRNA) for RNA interference in Sf-21 cell culture. Transfection with dsRNA only requires 1 h and the cells are usually recovered within 12 h. Suggestions for designing dsRNA are included in the methods. Furthermore, websites are provided for