Search
Invitrogen™
RiboPure™ RNA Purification Kit, yeast
For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expression자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| AM1926 | 50 Preps |
카탈로그 번호 AM1926
제품 가격(KRW)
699,000
온라인 행사
Ends: 31-Mar-2026
822,000할인액 123,000 (15%)
Each
수량:
50 Preps
제품 가격(KRW)
699,000
온라인 행사
Ends: 31-Mar-2026
822,000할인액 123,000 (15%)
Each
For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expression profiles. Each Ambion™ kit includes sufficient reagents for 50 purifications each from 107 to 108 yeast cells.
• Highest yields of total RNA available
• No enzymatic pretreatment required
• Includes DNA-free™ Reagents
It has traditionally been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Many protocols use enzymatic pretreatments that may affect gene expression patterns. The RiboPure™-Yeast RNA Isolation Kit uses no enzymatic pretreatments and thus does not alter expression profiles. It combines a simple and efficient glass bead/one step phenol–mediated disruption using Ambion's Vortex Bead Beater Adapter (see Accessory Products) followed by a glass fiber filter– based RNA purification step. After DNase treatment with the included DNA-free™ Reagents, the resulting RNA is exceptionally pure containing little to no genomic DNA (less than 0.05%). The kit has been used extensively at Ambion to isolate high-quality RNA from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, and Candida albicans (see Figure). The kit provides the highest yield of any method tested from each of these species (see Figure).
Accessory Products:
The Kit requires the use of the Vortex Adapter (Cat. No. AM10024), which turns a standard laboratory vortex into a bead beater. This adapter fits the Vortex Genie™ 2 and holds 12 tubes.
• Highest yields of total RNA available
• No enzymatic pretreatment required
• Includes DNA-free™ Reagents
It has traditionally been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Many protocols use enzymatic pretreatments that may affect gene expression patterns. The RiboPure™-Yeast RNA Isolation Kit uses no enzymatic pretreatments and thus does not alter expression profiles. It combines a simple and efficient glass bead/one step phenol–mediated disruption using Ambion's Vortex Bead Beater Adapter (see Accessory Products) followed by a glass fiber filter– based RNA purification step. After DNase treatment with the included DNA-free™ Reagents, the resulting RNA is exceptionally pure containing little to no genomic DNA (less than 0.05%). The kit has been used extensively at Ambion to isolate high-quality RNA from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, and Candida albicans (see Figure). The kit provides the highest yield of any method tested from each of these species (see Figure).
Accessory Products:
The Kit requires the use of the Vortex Adapter (Cat. No. AM10024), which turns a standard laboratory vortex into a bead beater. This adapter fits the Vortex Genie™ 2 and holds 12 tubes.
For Research Use Only. Not for use in diagnostic procedures.
사양
용리량50–100 μL
최종 제품 유형Total RNA
용도(애플리케이션)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
고처리량 호환성Not High-throughput Compatible (Manual)
정제 시간1.5 hr. (Including DNase treatment)
수량50 Preps
배송 조건Box 1: Dry Ice
Box 2: Room Temperature
Box 2: Room Temperature
시작 물질 양Up to 108 cells
수율Up to 300 μg
Isolation TechnologySpin Column (Glass Fiber Filter)
샘플 종류Yeast
Unit SizeEach
구성 및 보관
• 50 1.5-mL Screw Cap Tubes; store at room temperature
• 50 Filter Cartridges; room temperature
• 100 Collection Tubes (2 mL); room temperature
• 24 mL Lysis Buffer; 4°C
• 2.4 mL 10% SDS; 4°C
• 24 mL Phenol:Chloroform:IAA; 4°C
• 95 mL Binding Buffer; 4°C
• 35 mL Wash Solution 1; 4°C
• 50 mL Wash Solution 2/3 (concentrated); 4°C
• 5 mL Elution Solution; 4°C
• 80 g Zirconia Beads; -20°C
• 550 μL 10X DNase 1 Buffer; -20°C
• 200 μL DNase 1; -20°C
• 550 μL DNase Inactivation Reagent; -20°C
• 50 Filter Cartridges; room temperature
• 100 Collection Tubes (2 mL); room temperature
• 24 mL Lysis Buffer; 4°C
• 2.4 mL 10% SDS; 4°C
• 24 mL Phenol:Chloroform:IAA; 4°C
• 95 mL Binding Buffer; 4°C
• 35 mL Wash Solution 1; 4°C
• 50 mL Wash Solution 2/3 (concentrated); 4°C
• 5 mL Elution Solution; 4°C
• 80 g Zirconia Beads; -20°C
• 550 μL 10X DNase 1 Buffer; -20°C
• 200 μL DNase 1; -20°C
• 550 μL DNase Inactivation Reagent; -20°C