Ambion™ Recombinant RNase A for the production of plasmid DNA that is substantially free of host RNA is now available for research applications. For large batch sizes, the use of RNase is still the only effective technique applicable to remove host RNA. Other methods such as selective precipitation, gel filtration, chromatography, and endogenous E. coli RNase digestion have only been effective for small-scale or bench-scale purification. This new product was designed specifically to minimize the risk of introducing pathogens into bioprocessing. Recombinant RNase A is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and protease activity.