Invitrogen™

Silencer™ FAM-labeled Negative Control No. 1 siRNA

Ambion™ A fluorescently labeled negative control siRNA for the easy monitoring of siRNA uptake by fluorescence microscopy. Purified, annealed, and자세히 알아보기

카탈로그 번호	수량
AM4620	5 nmol

카탈로그 번호 AM4620

제품 가격(KRW)

350,000

キャンペーン価格

Ends: 31-Mar-2026

399,000

할인액 49,000 (12%)

5 nmol

제품 가격(KRW)

350,000

キャンペーン価格

Ends: 31-Mar-2026

399,000

할인액 49,000 (12%)

Ambion™ A fluorescently labeled negative control siRNA for the easy monitoring of siRNA uptake by fluorescence microscopy. Purified, annealed, and ready to use, the 5 nmol aliquot is supplied in a stable, dried format.

• Simplifies the monitoring of siRNA delivery
• Functionally tested in several common cell lines
• HPLC purified, duplexed, and ready to use

Visualize siRNA Uptake with Labeled siRNAs
Fluorescent labeled siRNAs allow for the direct observation siRNA cellular uptake, distribution, and localization. Fluorescently labeled siRNAs are commonly used to monitor transfection efficiency during optimization of transfection conditions. In general, siRNAs are rapidly taken up by and distributed throughout cells in as little as 4 hours. Fluorescent labeling of siRNAs allows for rapid monitoring of siRNA delivery by fluorescence microscopy, as opposed to unlabeled siRNAs, whose delivery must be monitored by target gene knockdown. Silencer™ FAM Labeled Negative Control #1 siRNAs provide an easy way to monitor siRNA uptake by chemical transfection. The sequences is identical to that of the Ambion Silencer™ Negative Control# 1 siRNA (SKU #AM4611), but with a fluorescein derivative FAM (5'-carboxyfluorescein; excitation max. = 494 nm, emission max. = 520 nm) on the 5' end of the sense strand, making it highly fluorescent when excited by the appropriate wavelength of light.

Accessory Products:
For the monitoring of siRNA delivery by electroporation, Silencer™ Cy™3 Labeled Negative Control #1 siRNA (SKU #AM4621) is recommended because of its brighter fluorescence signal visible over a broader pH range. Electroporation produces a more diffuse siRNA distribution pattern, resulting in a less intense fluorescence signal than with chemical transfection. Silencer™ control siRNAs are unmodified 21-mers; they are designed to be used with Silencer™ siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion™ Silencer™ Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

For Research Use Only. Not for use in diagnostic procedures.

용도(애플리케이션)Transfection, RNAi

라벨 또는 염료FAM

제품라인Silencer, Ambion

제품 유형siRNA

순도HPLC

수량5 nmol

배송 조건Dry Ice

제어 유형Negative Control

RNAi TypesiRNA

Unit SizeEach

구성 및 보관

The siRNA is provided dried down in a single tube, along with Nuclease-free Water for resuspension, and should be stored at –20°C.

자주 묻는 질문(FAQ)

How critical is it to include positive and negative controls in siRNA experiments?

A positive control siRNA such as the Silencer Select GAPDH siRNA will demonstrate efficient transfection and help to optimize transfection conditions. A negative control siRNA is necessary to serve as a baseline for gene expression.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

What are the positive and negative controls I should use with my Silencer or Silencer Select siRNA experiment?

We offer several negative and positive controls for both Silencer and Silencer Select siRNA.

For Silencer siRNA positive control:
- Silencer Firefly Luciferase (GL2 + GL3) siRNA (Cat. No. AM4629, 5nmol)
- Silencer GAPDH siRNA (human) (Cat. No AM4605, 5nmol; AM4633, 40 nmol)
- Silencer GAPDH siRNA (human, mouse, rat) (Cat. No. AM4624, 5 nmol; AM4631, 40 nmol; AM4632, 5 x 40 nmol)
- Silencer GFP (eGFP) siRNA (Cat. No. AM4626, 5 nmol)
- Silencer KIF11 (Eg5) siRNA (human, mouse, rat) (Cat. No. AM4639, 5 nmol)

If working with Fluorescently labeled Silencer siRNA:
- Silencer Cy-3 labeled GAPDH siRNA (human, mouse, rat) (Cat. No. AM4649, 5 nmol)
- Silencer FAM-labeled GAPDH siRNA (human, mouse, rat) (Cat. NO. AM4650, 5 nmol)

If working with Silencer siRNA in vivo ready:
- Silencer GAPDH Positive Control siRNA, in vivo ready (Cat. No. 4404025, 250 nmol)

For Silencer siRNA negative controls:
- Silencer Negative Control No. 1 siRNA (Cat. Nos. AM4611, 5 nmol; AM4635, 40 nmol; AM4636, 5 x 40 nmol)
- Silencer Negative Control No. 2 siRNA (Cat. Nos. AM4613, 5 nmol; AM4637, 30 nmol)
- Silencer Negative Control No. 3 siRNA (Cat. No. AM4615, 5 nmol)
- Silencer Negative Control No. 4 siRNA (Cat. No. AM4641, 5 nmol)
- Silencer Negative Control No. 5 siRNA (Cat. No. AM4642, 5 nmol)

If working with Fluorescently labeled Silencer siRNA:
- Silencer Cy 3-labeled Negative Control No. 1 siRNA (Cat. No AM4621, 5 nmol)
- Silencer FAM-labeled Negative Control No. 1 siRNA (Cat. No. AM4620, 5 nmol)

If working with Silencer siRNA in vivo ready:
- Silencer in vivo ready (Cat. No. 4404021)

For Silencer Select siRNA Positive control:
- Silencer Select GAPDH Positive Control siRNA (Cat. No. 4390849, 5 nmol; 4390850, 40 nmol; 4404024, 250 nmol)
- Silencer Select MALAT1 Positive Control siRNA for non-coding siRNA (Cat. No. 4455877, 5 nmol)

For Silencer Select siRNA negative control:
- Silencer Select Negative Control No. 1 siRNA (Cat. No. 4390843, 5 nmol; 4390844, 40 nmol)
- Silencer Select Negative Control No. 2 siRNA (Cat. No. 4390846, 5 nmol; 4390847, 40 nmol)

If working with Silencer Select siRNA in vivo ready:
- Silencer Select Negative Control No. 1 siRNA, in vivo ready (Cat. No. 4404020, 250nmol)

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.