Lambda Hind III dsDNA Markers

Ambion® Lambda DNA is digested to completion with Hind III. This generates fragments of the sizes 23130, 9416, 6557, 4361,자세히 알아보기

카탈로그 번호	수량
AM7720	200 ug

카탈로그 번호 AM7720

제품 가격(KRW)

200 ug

Ambion® Lambda DNA is digested to completion with Hind III. This generates fragments of the sizes 23130, 9416, 6557, 4361, 2322, 2027, 564, and 125 bp. These DNA molecular mass markers are supplied with a protocol describing easy labeling of the markers with radioisotopes. They can also be stained with ethidium bromide. One tube containing 200 μg is provided.

For Research Use Only. Not for use in diagnostic procedures.

형태Liquid

젤 호환성Agarose Gels

제품 유형DNA Marker

수량200 ug

배송 조건Room Temperature

크기 범위125 to 23.1 kb

농도0.5 mg/mL

제품라인Ambion

Unit SizeEach

구성 및 보관

Store at –20°C.

자주 묻는 질문(FAQ)

How can I quantitate DNA on an agarose gel?

For fragments between 100 bp and 2,000 bp, use the Low DNA Mass Ladder. 4 µL of the Low DNA Mass Ladder corresponds to 10, 20, 40, 80, 120, and 200 ng respectively of fragments, 100, 200, 400, 800, 1,200 and 2,000 bp.

For fragments between 1,000 bp and 10,000 bp use the High DNA Mass Ladder. 4 µL of the High DNA Mass Ladder corresponds to 20, 40, 60, 80, 120, 200 ng respectively of fragments 1,000, 2,000, 3,000, 4,000, 6,000, and 10,000 bp.

To calculate the amount of DNA in 1 µg of the Lambda/Hind III standard:
(size of band in bp/48503 bp) X 1 µg = µg of DNA in band A

For example, to calculate the amount of DNA in the 564 bp band of the Lambda/Hind III standard:
(564/48,503) X 1 µg = 0.011 µg or 11 ng of DNA in the 564 bp band.