BTC, AM is a cell-permeant coumarin benzothiazole-based Ca2+ indicator. It exhibits a shift in excitation maximum from about 480 nm자세히 알아보기
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카탈로그 번호
수량
B6791
100 μg
카탈로그 번호 B6791
제품 가격(KRW)
488,000
Exklusiv online
Ends: 31-Mar-2026
573,000
할인액 85,000 (15%)
Each
카트에 추가하기
수량:
100 μg
제품 가격(KRW)
488,000
Exklusiv online
Ends: 31-Mar-2026
573,000
할인액 85,000 (15%)
Each
카트에 추가하기
BTC, AM is a cell-permeant coumarin benzothiazole-based Ca2+ indicator. It exhibits a shift in excitation maximum from about 480 nm to 400 nm upon binding Ca2+, which permits ratiometric measurements that are essentially independent of uneven dye loading cell thickness, photobleaching and dye leakage. BTC has high selectivity for Ca2+ and a Kd ∼7 μM enabling quantitation of high intracellular Ca2+ levels.
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법Fluorescence
수량100 μg
배송 조건Room Temperature
용도(애플리케이션)Cell Viability and Proliferation
용도 (장비)Flow Cytometer
제품 유형Calcium Indicator
Unit SizeEach
구성 및 보관
Store in freezer -5°C to -30°C and protect from light.
인용 및 참조 문헌 (18)
인용 및 참조 문헌
Abstract
AM-loading of fluorescent Ca2+ indicators into intact single fibers of frog muscle.
Authors:Zhao M, Hollingworth S, Baylor SM
Journal:Biophys J
PubMed ID:9168048
'The AM loading of a number of different fluorescent Ca2+ indicators was compared in intact single fibers of frog muscle. Among the 13 indicators studied, loading rates (the average increase in the fiber concentration of indicator per first 60 min of loading) varied approximately 100-fold, from approximately 3 microM/h to ... More
Significance of Na/Ca exchange for Ca2+ buffering and electrical activity in mouse pancreatic beta-cells.
Authors:Gall D, Gromada J, Susa I, Rorsman P, Herchuelz A, Bokvist K
Journal:Biophys J
PubMed ID:10096898
'We have combined the patch-clamp technique with microfluorimetry of the cytoplasmic Ca2+ concentration ([Ca2+]i) to characterize Na/Ca exchange in mouse beta-cells and to determine its importance for [Ca2+]i buffering and shaping of glucose-induced electrical activity. The exchanger contributes to Ca2+ removal at [Ca2+]i above 1 microM, where it accounts for ... More
Photolysis-induced suppression of inhibition in rat hippocampal CA1 pyramidal neurons.
Authors:Wang J, Zucker RS
Journal:J Physiol
PubMed ID:11410632
'1. Whole cell patch clamp recording, Ca(2+) measurement with ratiometric fluorescent dyes and photolysis of caged Ca(2+) were combined to investigate the depolarization- and photolysis-induced suppression of inhibition (DSI and PSI) in rat hippocampal CA1 pyramidal cells. 2. A 5-s depolarization from -70 mV to 0 mV or a 6-s ... More
'Cytosolic calcium ([Ca2+]i) is an important mediator of neuronal signal transduction, participating in diverse biochemical reactions that elicit changes in synaptic efficacy, metabolic rate, and gene transcription. Excessive [Ca2+]i also has been implicated as a cause of acute neuronal injury, although measurement of [Ca2+]i in living neurons by fluorescent calcium ... More
Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC.
Authors:Hyrc KL, Bownik JM, Goldberg MP
Journal:Cell Calcium
PubMed ID:10756974
'Accurate measurement of elevated intracellular calcium levels requires indicators with low calcium affinity and high selectivity. We examined fluorescence spectral properties and ionic specificity of three low-affinity, ratiometric indicators structurally related to Fura-2: mag-Fura-2 (furaptra), Fura-2FF, and BTC. The indicators differed in respect to their excitation wavelengths, affinity for Ca2+ ... More