Bolt™ Transfer Buffer (20X)
Bolt™ Transfer Buffer (20X)
Invitrogen™

Bolt™ Transfer Buffer (20X)

Bolt Transfer Buffer (20X) is used to transfer proteins from Bolt Bis-Tris Plus gels to membranes for western blotting. It자세히 알아보기
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카탈로그 번호수량
BT0006125mL
BT000611L
카탈로그 번호 BT0006
제품 가격(KRW)
44,000
온라인 행사
Ends: 31-Dec-2025
51,000
할인액 7,000 (14%)
Each
카트에 추가하기
수량:
125mL
제품 가격(KRW)
44,000
온라인 행사
Ends: 31-Dec-2025
51,000
할인액 7,000 (14%)
Each
카트에 추가하기
Bolt Transfer Buffer (20X) is used to transfer proteins from Bolt Bis-Tris Plus gels to membranes for western blotting. It maintains the neutral pH environment established during electrophoresis. The neutral pH protects against modification of amino acid side chains and is compatible with N-terminal protein sequencing using Edman degradation.

Bolt Antioxidant may be used with Bolt Transfer Buffer to enhance transfer of reduced proteins to membranes.

See all available buffers and reagents available for SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.
사양
버퍼Transfer Buffers
농도20 X
형태Liquid
젤 호환성Bolt™ Bis-Tris Plus Gels
제품라인Bolt
제품 유형Western Blot Transfer Buffer
수량125mL
부피(미터법)125 ml
Unit SizeEach
구성 및 보관
Store at 4 to 25°C.

자주 묻는 질문(FAQ)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the shelf life for Bolt gels?

The shelf life for Bolt gels is similar to that for NuPAGE gels: 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

View all Bolt™ Transfer Buffer (20X), 125mL FAQs