The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay provides a fast, sensitive, non-toxic and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy and high-content imaging. Click-iT® AHA is an amino acid analog of methionine containing an azido moiety. Similar to 35S-methionine, Click-iT® AHA is fed to cultured cells and incorporated into proteins during active protein synthesis. Detection of the incorporated amine acid utilizes a chemoselectiove ligation or “click" reaction between an azide and an alkyne, where the azido modified protein is detected with the green-fluorescent Alexa Fluor® 488 alkyne. The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay has been successfully tested in A549 and U-2 OS cells with a variety of reagents that inhibit protein synthesis including cycloheximide, anisomycin and puromycin in both dose response and Min⁄Max format.
For Research Use Only. Not for use in diagnostic procedures.
사양
색상
Blue, Green
검출 방법
Fluorescent
용도(애플리케이션)
High-content Screening (HCS)
용도 (장비)
Fluorescence Microscope, High Content Analysis Instrument
제품 유형
HCS Assay
염료 유형
Alexa Fluor™ 488, Hoechst 33258
방출
UV, Visible
형식
96-well plate
그린 기능
Less hazardous
제품라인
Alexa Fluor™, Click-iT™, Molecular Probes™
배송 조건
Room Temperature
구성 및 보관
The kit contains sufficient reagents for performing 2 plates in a 96-well plate format.
Store at ≤-20°C, desiccate and protect from light.