CellROX™ Deep Red Reagent, for oxidative stress detection

인용 및 참조 문헌 (52)

Invitrogen™

CellROX™ Deep Red Reagent, for oxidative stress detection

CellROX™ Deep Red Reagent는 살아있는 세포와 고정 세포 이미지 모두에서 세포 산화 스트레스 (oxidative stress)를 측정하는 새로운 형광 probe로 ∼644/665자세히 알아보기

카탈로그 번호	수량
C10422	5 x 50 μL

카탈로그 번호 C10422

제품 가격(KRW)

793,000

Online offer

Ends: 31-Dec-2025

881,000

할인액 88,000 (10%)

카트에 추가하기

5 x 50 μL

제품 가격(KRW)

793,000

Online offer

Ends: 31-Dec-2025

881,000

할인액 88,000 (10%)

카트에 추가하기

CellROX™ Deep Red Reagent는 살아있는 세포와 고정 세포 이미지 모두에서 세포 산화 스트레스 (oxidative stress)를 측정하는 새로운 형광 probe로 ∼644/665 nm에서 absorption/emission이 최대입니다. 이 세포 투과성 dye는 환원 상태에서는 형광을 나타내지 않지만 활성 산소종(reactive oxygen species, ROS)으로 산화되면 밝은 형광을 나타냅니다.

CellROX™ Deep Red Reagent의 특징:

• 세포에서 산화 스트레스 (oxidative stress) 검출에 최적화된 probe
• 다른 live cell dye와 GFP에 사용할 수 있는 간단한 protocol
• Live cell 형광 이미지와 포름알데하이드 고정법에 모두 사용가능

기질 특이적
CellROX™ Deep Red 시약은 ∼644/665 nm에서 absorption/emission이 최대인 새로운 세포 투과성 dye입니다. CellROX™ Deep Red 시약은 환원 상태에서는 형광을 나타내지 않지만 활성 산소종(reactive oxygen species, ROS)으로 산화되면 ∼665 nm에서 emission이 최대인 형광을 발하여 형광 이미지, 고함량 이미지, 형광 플레이트 판독기 또는 flow cytometry로 측정할 수 있습니다.

CellROX™ Deep Red Reagent의 산화 스트레스 검출
산화 스트레스는 활성 산소종(ROS)의 생산과 세포 내 제거의 불일치로 야기됩니다. ROS는 염증, 죽상경화증, 노화, 나이 관련 퇴행성 장애와 같은 여러 질병의 진행에 중요한 역할을 합니다. CellROX™ Deep Red Reagent는 ROS와 반응하여 밝은 형광을 발하여 산화 스트레스 (oxidative stress)를 검출할 수 있습니다.

간단하고 확실한 Protocol
이 시약을 사용하는 protocol은 간단하며, 짙은 적색의 밝은 형광 신호는 다른 생존 세포 염색 및 GFP와 호환되어 세포 독성 및 세포 사멸과 관련된 파라미터를 포함하여 다양한 세포 현상을 측정하는 다중 형광 분석(multiplex fluorescence assay)에 유용합니다(아래 그림 4). 또한 H2DCFDA같은 다른 여러 ROS 센서와 달리 CellROX™ Deep Red 신호는 포름알데하이드 고정 후에도 유지되어 분석 활용도가 높고 ROS 검출에 사용하는 기존 dye를 기반으로 하는 경우보다 개선된 workflow를 제공합니다.

For Research Use Only. Not for use in diagnostic procedures.

색상Deep Red

농도2.5 mM stabilized solution in DMSO

용도(장비)Imaging, HCS, Cytometer

형식Liquid

수량5 x 50 μL

검출 방법Live Cell Imaging

Excitation/Emission644/665 nm

IndicatorOxidative stress

제품라인CellROX

Unit SizeEach

구성 및 보관

Store at ≤–20°C. Protect from light and desiccate.

자주 묻는 질문(FAQ)

I want to assay cells for reactive oxygen species using carboxy-H2DCFDA, but I want to do so with a plate reader instead of microscope. Will it work?

It has been done. The problem is that plate readers are less sensitive than microscopes, with far less signal-to-background difference. It is worth trying, but first optimize concentrations and loading times with control cells, use a plate with little to no autofluorescence, and possibly optimize the gain setting in order to get the best signal possible. But don't expect the same sensitivity, even with optimization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have GFP-transfected cells and need to label for reactive oxygen species. Can I use H2DCFDA?

This is not recommended as the two dyes overlap in the emission wavelength. There are other ROS reagents available in different wavelengths, such as CellROX Deep Red, which emits in the far-red range (665 nm), or dihydroethidium, which is emits in the visible red range (620 nm).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need a formaldehyde-fixable reactive oxygen species detection assay. Is H2 DCFDA fixable?

H₂DCFDA and similar derivatives are not fixable. The same goes for dihydroethidium and dihydrorhodamine. However, CellROX Deep Red and CellROX Green are retained for a limited time upon fixation with formaldehyde. CellROX Green may be retained upon subsequent Triton X-100 permeabilization. Avoid the use of any acetone or alcohol-based fixatives or fixatives that include alcohol, such as formalin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What dyes can I use to detect reactive oxygen species (ROS) in my bacteria?

Many dyes that are used on mammalian cells have also been shown to be useful in bacterial cells. For example, CellROX Deep Red Reagent has been shown to work in B. subtilis (see Reference: http://www-brs.ub.ruhr-uni-bochum.de/netahtml/HSS/Diss/RaatschenNadja/diss.pdf). If you are interested in a particular dye, but are not sure if it will work on your bacteria, literature searches are the best way to check to see if it has been tested. If not, then it may be worth testing yourself.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am trying to label my cells with a reactive oxygen species (ROS) indicator dye, but I am not seeing a significant difference in signal. What could be happening?

First, make sure you have both a negative (untreated) and positive (ROS-induced) sample to compare. A good positive control can be the use of 100 µM menadione for one hour or 50 µM nefazodone for 24 hours. H2O2 can also be used, though it does not work well for CellROX dyes. Some dyes, such as H2DCFDA, require esterase cleavage, so don't incubate in the presence of serum (which contains esterases that can prematurely cleave the dye). If your positive control does not show significant change compared to the negative control, try increasing the concentration and label time for the dye. Our manuals give starting recommendations. Be sure to image your live cells as soon as possible. Only two dyes (CellROX Green and CellROX Deep Red) are retained with formaldehyde fixation. Finally, make sure you are using filters and instrument settings to match the excitation and emission spectra of the dye.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (52)

인용 및 참조 문헌

Abstract

The airway epithelium nucleotide-binding domain and leucine-rich repeat protein 3 inflammasome is activated by urban particulate matter.

Authors:Hirota JA, Hirota SA, Warner SM, Stefanowicz D, Shaheen F, Beck PL, Macdonald JA, Hackett TL, Sin DD, Van Eeden S, Knight DA,

Journal:J Allergy Clin Immunol

PubMed ID:22227418

The airway epithelium is the first line of defense against inhaled insults and therefore must be capable of coordinating appropriate inflammatory and immune responses. We sought to test the hypothesis that the nucleotide-binding domain and leucine-rich repeat protein 3 (NLRP3) inflammasome, an intracellular danger-sensing complex, plays a critical role in ... More

Combination Small Molecule MEK and PI3K Inhibition Enhances Uveal Melanoma Cell Death in a Mutant GNAQ- and GNA11-Dependent Manner.

Authors:Khalili JS, Yu X, Wang J, Hayes BC, Davies MA, Lizee G, Esmaeli B, Woodman SE,

Journal:Clin Cancer Res

PubMed ID:22733540

Activating Q209L/P mutations in GNAQ or GNA11 (GNAQ/11) are present in approximately 80% of uveal melanomas. Mutant GNAQ/11 are not currently therapeutically targetable. Inhibiting key down-stream effectors of GNAQ/11 represents a rational therapeutic approach for uveal melanomas that harbor these mutations. The mitogen-activated protein/extracellular signal-regulated kinase/mitogen-activated protein kinase (MEK/MAPK) and ... More

Mutant BRAF induces DNA strand breaks, activates DNA damage response pathway, and up-regulates glucose transporter-1 in nontransformed epithelial cells.

Authors:Sheu JJ, Guan B, Tsai FJ, Hsiao EY, Chen CM, Seruca R, Wang TL, Shih IeM,

Journal:Am J Pathol

PubMed ID:22227015

'Although the oncogenic functions of activating BRAF mutations have been clearly demonstrated in human cancer, their roles in nontransformed epithelial cells remain largely unclear. Investigating the cellular response to the expression of mutant BRAF in nontransformed epithelial cells is fundamental to the understanding of the roles of BRAF in cancer ... More

Mechanisms of programmed cell death signaling in hair cells and support cells post-electrode insertion trauma.

Authors:Eshraghi AA, Lang DM, Roell J, Van De Water TR, Garnham C, Rodrigues H, Guardiola M, Gupta C, Mittal J,

Journal:

PubMed ID:25761716

'Programmed cell death (PCD) initially starts in the support cells (SCs) after electrode insertion trauma (EIT), followed by PCD in hair cells (HCs). Activation of caspase-3 was observed only in SCs. Protecting both SCs and HCs with selective otoprotective drugs at an early stage post implantation may help to preserve ... More

OPA1 Mutation and Late-Onset Cardiomyopathy: Mitochondrial Dysfunction and mtDNA Instability.

Authors:Chen L, Liu T, Tran A, Lu X, Tomilov AA, Davies V, Cortopassi G, Chiamvimonvat N, Bers DM, Votruba M, Knowlton AA,

Journal:J Am Heart Assoc

PubMed ID:23316298

'Mitochondrial fusion protein mutations are a cause of inherited neuropathies such as Charcot-Marie-Tooth disease and dominant optic atrophy. Previously we reported that the fusion protein optic atrophy 1 (OPA1) is decreased in heart failure. We investigated cardiac function, mitochondrial function, and mtDNA stability in a mouse model of the disease ... More

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