OPP (O-propargyl-puromycin) is an alkyne analog of puromycin that is efficiently incorporated into newly translated proteins in complete methionine-containing media.자세히 알아보기
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C10459
5 mg
카탈로그 번호 C10459
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5 mg
OPP (O-propargyl-puromycin) is an alkyne analog of puromycin that is efficiently incorporated into newly translated proteins in complete methionine-containing media. When used with Click-iT® Plus OPP Alexa Fluor® Protein Synthesis assays or Alexa Fluor® Picolyl Azide toolkits, OPP provides a fast, sensitive, and non-radioactive method for the detection of protein synthesis using fluorescence microscopy, high-content imaging, or flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.
'The search for specific productivity (qP) determinants in Chinese hamster ovary (CHO) cells has been the focus of the biopharmaceutical cell line engineering efforts aimed at creating ' ... More
Suppression of the GTPase-activating protein RGS10 increases Rheb-GTP and mTOR signaling in ovarian cancer cells.
The regulator of G protein signaling 10 (RGS10) protein is a GTPase activating protein that accelerates the hydrolysis of GTP and therefore canonically inactivates G proteins, ultimately terminating signaling. Rheb is a small GTPase protein that shuttles between its GDP- and GTP-bound forms to activate mTOR. Since RGS10 suppression augments ... More
Nucleolar Enrichment of Brain Proteins with Critical Roles in Human Neurodevelopment.
Authors:Slomnicki LP, Malinowska A, Kistowski M, Palusinski A, Zheng JJ, Sepp M, Timmusk T, Dadlez M, Hetman M,
Journal:Mol Cell Proteomics
PubMed ID:27053602
'To study nucleolar involvement in brain development, the nuclear and nucleolar proteomes from the rat cerebral cortex at postnatal day 7 were analyzed using LC-MS/iTRAQ methodology. Data of the analysis are available via ProteomeXchange with identifier PXD002188. Among 504 candidate nucleolar proteins, the overrepresented gene ontology terms included such cellular ... More
The VHL short variant involves in protein quality control.
Authors:Liu Y, Yang H, Zuo F, Chen L,
Journal:Gene
PubMed ID:27196060
'The von Hippel-Lindau (VHL) is the most important and frequently mutated gene in human clear cell renal cell carcinoma (ccRCC). In contrast to its long counterpart, the internal translational variant of VHL protein (VHLs) is evolutionarily conserved. Herein we present evidence that VHLs associates with ribosome complex via interaction with ... More
Requirement of Neuronal Ribosome Synthesis for Growth and Maintenance of the Dendritic Tree.
Authors:Slomnicki LP, Pietrzak M, Vashishta A, Jones J, Lynch N, Elliot S, Poulos E, Malicote D, Morris BE, Hallgren J, Hetman M,
Journal:J Biol Chem
PubMed ID:26757818
'The nucleolus serves as a principal site of ribosome biogenesis but is also implicated in various non-ribosomal functions, including negative regulation of the pro-apoptotic transcription factor p53. Although disruption of the nucleolus may trigger the p53-dependent neuronal death, neurotoxic consequences of a selective impairment of ribosome production are unclear. Here, ... More