Calcein AM, Cell-permeant Green and Blue Dyes
Calcein AM, Cell-permeant Green and Blue Dyes
인용 및 참조 문헌 (15)
Invitrogen™

Calcein AM, Cell-permeant Green and Blue Dyes

Calcein AM은 대부분의 진핵 세포에서 세포 생존율을 측정하는 데 사용할 수 있는 세포 투과성 dye입니다. 살아있는 세포에서 비형광 calcein AM은자세히 알아보기
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카탈로그 번호제품 유형수량색상
C1429Calcein Blue, AM1 mgBlue
C3100MPCalcein, AM20 x 50 μgGreen
C1430Calcein, AM1 mgGreen
C3099Calcein, AM1 mLGreen
C34852Calcein Green, AM20 x 50 μgGreen
C481Calcein, high purity100 mgGreen
카탈로그 번호 C1429
제품 가격(KRW)
261,000
온라인 행사
Ends: 31-Mar-2026
298,000
할인액 37,000 (12%)
Each
카트에 추가하기
제품 유형:
Calcein Blue, AM
수량:
1 mg
색상:
Blue
제품 가격(KRW)
261,000
온라인 행사
Ends: 31-Mar-2026
298,000
할인액 37,000 (12%)
Each
카트에 추가하기
Calcein AM은 대부분의 진핵 세포에서 세포 생존율을 측정하는 데 사용할 수 있는 세포 투과성 dye입니다. 살아있는 세포에서 비형광 calcein AM은 세포간 esterase에 의해 acetoxymethyl ester 가수분해 후 녹색 형광 calcein으로 전환됩니다. 이 염료는 자사의 특수 패키지(C-3100)에도 들어있으며 DMSO(C-3099)에 녹일 수 있습니다. 파장 길이가 긴 dye 로는 새로운 CellTrace calcein red-orange AM (C-34851)을 확인하십시오.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 투과성Cell-permeant
염료 유형Other Label(s) or Dye(s)
여기/방출322/437 nm
분자량465.41
수량1 mg
시약 유형Cell Tracker Compounds, Cell Labeling Reagents
배송 조건Room Temperature
타겟 효소Esterase
색상Blue
Emission449
용도(애플리케이션)Cell Tracing, Cell Tracker
용도 (장비)Fluorescence Microscope
제품 유형Calcein Blue, AM
Unit SizeEach
구성 및 보관
Store in freezer (-5°C to -30°C) and protect from light.

자주 묻는 질문(FAQ)

I need a general cytoplasmic stain that does not overlap with the GFP in my cells. What do you recommend?

Calcein AM, a green dye, is typically used as a general cytoplasmic stain, but not recommended with GFP-positive cells. For GFP-expressing cells there are other colors available: Calcein Blue AM, Calcein Violet AM, and Calcein Red-Orange AM. The retention time of these dyes in live cells is dependent upon the inherent properties of the cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I stained two populations of cells, one with CellTracker Green and the other with CellTracker Red, but it looks like there may be crossover of the red dye to the green cells. What is going on?

One possibility is that there is spectral bleedthrough between the dyes. Be sure to check the single-color samples by imaging the red cells in green and imaging the green cells in red, using the optimal imaging settings for the other color. If you see bleedthrough with these controls, then you will have to reduce the dye label concentration to reduce the brightness of the dyes, or choose dyes that are farther apart spectrally. If the issue isn’t bleedthrough, another possibility is that the cells were not adequately washed after staining, allowing some unincorporated dye to remain and label the other cells after they were introduced. Extending washes and wash times should help with this.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I stained my cells with Calcein, AM, but the signal went away after I fixed my cells. Why is this?

Calcein, AM diffuses into cells, the 'AM' moiety is cleaved by cellular esterases, and then the dye molecules are observed in the cytoplasm without binding to anything. This gives a 'whole cell' stain. It also means that the dyes are not crosslinked with aldehyde-based fixation and thus will be lost upon fixation. Additionally, any disruption of plasma membrane, such as with detergents or trypsinization, will lead to leakage of the dye from the cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I'm trying to stain my cells with CellTracker dyes or CFDA SE, but I'm not seeing much signal. What can I do?

First, make sure you aren’t staining in the presence of serum, since serum can have esterase activity that can prematurely cleave the AM group on these dyes, preventing entry into cells. After staining, it’s okay to return the cells to medium containing serum. After this, you can try increasing the concentration and label time to get a higher intensity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I like how calcein dyes label the whole cell. How long can I track my cells with them, and can I fix them?

Calcein dyes diffuse into cells, the 'AM' moiety is cleaved by cellular esterases and then are observed in the cytoplasm without binding to anything. This provides a 'whole cell' label. Calcein dyes may be pumped out by normal cellular efflux mechanisms, sometimes within a very short time, especially for cell types that may exhibit drug resistance, unless the efflux is inhibited (such as with probenecid). The dyes are not crosslinked with aldehyde-based fixation, unlike protein-binding CellTracker dyes, and thus will be lost upon fixation. Additionally, any disruption of plasma membrane, such as with detergents or trypsinization, will lead to leakage of the dyes from the cell.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

인용 및 참조 문헌 (15)

인용 및 참조 문헌
Abstract
Whole animal cell sorting of Drosophila embryos.
Authors:Krasnow MA, Cumberledge S, Manning G, Herzenberg LA, Nolan GP
Journal:Science
PubMed ID:1898782
'Use of primary culture cells has been limited by the inability to purify most types of cells, particularly cells from early developmental stages. In whole animal cell sorting (WACS), live cells derived from animals harboring a lacZ transgene are purified according to their level of beta-galactosidase expression with a fluorogenic ... More
UVA-induced apoptosis studied by the new apo/necro-Comet-assay which distinguishes viable, apoptotic and necrotic cells.
Authors:Morley N, Rapp A, Dittmar H, Salter L, Gould D, Greulich KO, Curnow A,
Journal:Mutagenesis
PubMed ID:16500949
An adaptation of the Comet-assay was developed which enables the discrimination of viable, apoptotic and necrotic single cells by use of the common Annexin-V staining and a dye exclusion test on the cells already embedded in agarose gel on glass slides. Membrane integrity (Ethidium-Homodimer exclusion), cellular esterase activity (Calcein blue-AM) ... More
Advantages and limitations of commonly used methods to assay the molecular permeability of gap junctional intercellular communication.
Authors:Abbaci M, Barberi-Heyob M, Blondel W, Guillemin F, Didelon J,
Journal:Biotechniques
PubMed ID:18611167
The role of gap junctional intercellular communication (GJIC) in regulation of normal growth and differentiation is becoming increasingly recognized as a major cellular function. GJIC consists of intercellular exchange of low molecular weight molecules, and is the only means for direct contact between cytoplasms of adjacent animal cells. Disturbances of ... More
Early intermediates in HIV-1 envelope glycoprotein-mediated fusion triggered by CD4 and co-receptor complexes.
Authors:Dimitrov AS, Xiao X, Dimitrov DS, Blumenthal R
Journal:J Biol Chem
PubMed ID:11397808
An early step in the process of HIV-1 entry into target cells is the activation of its envelope glycoprotein (GP120-GP41) to a fusogenic state upon binding to target cell CD4 and cognate co-receptor. Incubation of human immunodeficiency virus (HIV)-1 Env-expressing cells with an excess of CD4 and co-recepeptor-bearing target cells ... More
RANK ligand-induced elevation of cytosolic Ca2+ accelerates nuclear translocation of nuclear factor kappa B in osteoclasts.
Authors:Komarova SV, Pilkington MF, Weidema AF, Dixon SJ, Sims SM
Journal:J Biol Chem
PubMed ID:12496256
RANK ligand (RANKL) induces activation of NFkappaB, enhancing the formation, resorptive activity, and survival of osteoclasts. Ca(2+) transduces many signaling events, however, it is not known whether the actions of RANKL involve Ca(2+) signaling. We investigated the effects of RANKL on rat osteoclasts using microspectrofluorimetry and patch clamp. RANKL induced ... More
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