Calcein AM, Cell-permeant Green and Blue Dyes

인용 및 참조 문헌 (632)

Invitrogen™

Calcein AM, Cell-permeant Green and Blue Dyes

Name: Calcein AM, Cell-permeant Green and Blue Dyes
SKU: C1430
Price: 488000 KRW

Calcein AM은 대부분의 진핵 세포에서 세포 생존율을 측정하는 데 사용할 수 있는 세포 투과성 dye입니다. 살아있는 세포에서 비형광 calcein AM은자세히 알아보기

보기 방식 변경

카탈로그 번호	제품 유형	수량	색상
C1430	Calcein, AM	1 mg	Green
C3100MP	Calcein, AM	20 x 50 μg	Green
C3099	Calcein, AM	1 mL	Green
C1429	Calcein Blue, AM	1 mg	Blue
C34852	Calcein Green, AM	20 x 50 μg	Green
C481	Calcein, high purity	100 mg	Green

6 개 옵션

카탈로그 번호 C1430

제품 가격(KRW)

488,000

온라인 행사

Ends: 31-Mar-2026

542,000

할인액 54,000 (10%)

Each

카트에 추가하기

제품 유형:

Calcein, AM

수량:

1 mg

색상:

Green

제품 가격(KRW)

488,000

온라인 행사

Ends: 31-Mar-2026

542,000

할인액 54,000 (10%)

Each

카트에 추가하기

Calcein AM은 대부분의 진핵 세포에서 세포 생존율을 측정하는 데 사용할 수 있는 세포 투과성 dye입니다. 살아있는 세포에서 비형광 calcein AM은 세포간 esterase에 의해 acetoxymethyl ester 가수분해 후 녹색 형광 calcein으로 전환됩니다. 이 염료는 자사의 특수 패키지(C-3100)에도 들어있으며 DMSO(C-3099)에 녹일 수 있습니다. 파장 길이가 긴 dye 로는 새로운 CellTrace calcein red-orange AM (C-34851)을 확인하십시오.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.

For Research Use Only. Not for use in diagnostic procedures.

사양

세포 투과성Cell-permeant

염료 유형Other Label(s) or Dye(s)

수량1 mg

시약 유형Cell Tracker Compounds, Cell Labeling Reagents

배송 조건Room Temperature

타겟 효소Esterase

색상Green

Emission517

Excitation Wavelength Range494 nm

용도(애플리케이션)Cell Tracing, Cell Tracker

용도 (장비)Fluorescence Microscope

제품 유형Calcein, AM

Unit SizeEach

구성 및 보관

Store in freezer (-5°C to -30°C) and protect from light.

자주 묻는 질문(FAQ)

I would like a dye to load in live cells such that it will self-quench at a high concentration, but if the cell dies, the dye will be released and unquenched. Do you have anything like that?

Yes. This is commonly done with calcein AM or FDA (fluorescein diacetate). These dyes will not fluoresce until cleaved by esterases. After modification by esterases and at very high concentrations, they will self-quench. Upon disruption of the plasma membrane, or cell death, the dye will be released into the extracellular medium, and become unquenched. Concentration and incubation time must be optimized to obtain adequate quenching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to load liposomes with calcein. Should I use the AM form or the non-AM form?

Calcein, AM requires esterase cleavage of the acetoxymethyl (AM) ester to become fluorescent. Liposomes don't have esterases unless specifically constructed to include the enzyme. The water-soluble, non-AM form of calcein (Cat. No. C481), does not require esterase cleavage to be fluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to do a cell migration study for around 4 hours and need to fluorescently label the cells with a dye. What do you recommend?

Calcein, AM and FDA (fluorescein diaceate) are examples of some dyes used for this application. Since these dyes are not incorporated or covalently attached to any cellular components, they may have a short retention time as some cell types may actively efflux the dye out of the cells. The CellTracker and CellTrace dyes include either a mild thiol-reactive chloromethyl group or amine-reactive succinnimidyl ester group to allow for covalent binding to cellular components, providing for better retention. As with any reagent, one should empirically determine retention times for the cell type used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am doing a Live/Dead assay using Calcein, AM, for live cells and ethidium homodimer-1 for dead cells. Can I fix the cells after labeling and retain the staining?

This is not recommended. Neither Calcein nor ethidium homodimer-1 bind to any cellular components upon fixation. There is no guarantee that the dyes will be retained upon fixation or any subsequent wash steps. We recommend scoring for live and dead cells as soon as possible after staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need a general cytoplasmic stain that does not overlap with the GFP in my cells. What do you recommend?

Calcein AM, a green dye, is typically used as a general cytoplasmic stain, but not recommended with GFP-positive cells. For GFP-expressing cells there are other colors available: Calcein Blue AM, Calcein Violet AM, and Calcein Red-Orange AM. The retention time of these dyes in live cells is dependent upon the inherent properties of the cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Calcein AM, Cell-permeant Green and Blue Dyes FAQs

인용 및 참조 문헌 (632)

인용 및 참조 문헌

Abstract

Nitric oxide mediates natural polyphenol-induced Bcl-2 down-regulation and activation of cell death in metastatic B16 melanoma.

Authors:Ferrer P,Asensi M,Priego S,Benlloch M,Mena S,Ortega A,Obrador E,Esteve JM,Estrela JM

Journal:The Journal of biological chemistry

PubMed ID:17135264

Cloning and expression of murine sister of P-glycoprotein reveals a more discriminating transporter than MDR1/P-glycoprotein.

Authors:Lecureur V,Sun D,Hargrove P,Schuetz EG,Kim RB,Lan LB,Schuetz JD

Journal:Molecular pharmacology

PubMed ID:10617675

Large-scale chemical dissection of mitochondrial function.

Authors:Wagner BK,Kitami T,Gilbert TJ,Peck D,Ramanathan A,Schreiber SL,Golub TR,Mootha VK

Journal:Nature biotechnology

PubMed ID:18297058

Mitochondrial oxidative phosphorylation (OXPHOS) is central to physiology and disease pathogenesis. To systematically investigate its activity and regulation, we performed a wide range of assays of OXPHOS physiology and nuclear and mitochondrial gene expression across 2490 chemical perturbations in muscle cells. Through mining of the resulting compendium, we discovered that: ... More

Modification of the cytoplasmic domain of influenza virus hemagglutinin affects enlargement of the fusion pore.

Authors:Kozerski C,Ponimaskin E,Schroth-Diez B,Schmidt MF,Herrmann A

Journal:Journal of virology

PubMed ID:10906206

The fusion activity of chimeras of influenza virus hemagglutinin (HA) (from A/fpv/Rostock/34; subtype H7) with the transmembrane domain (TM) and/or cytoplasmic tail (CT) either from the nonviral, nonfusogenic T-cell surface protein CD4 or from the fusogenic Sendai virus F-protein was studied. Wild-type or chimeric HA was expressed in CV-1 cells ... More

Authors:

Journal:

PubMed ID:10891486

View all Calcein AM, Cell-permeant Green and Blue Dyes citations