Search
인용 및 참조 문헌 (66)
Invitrogen™
Calcium Calibration Buffer Kit #1, zero and 10 mM CaEGTA
The Calcium Calibration Buffer Kits are designed to help determine the dissociation constant (Kd) of fluorescent Ca2+ indicators using a자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| C3008MP | 1 Kit |
카탈로그 번호 C3008MP
제품 가격(KRW)
574,000
온라인 행사
Ends: 31-Mar-2026
675,000할인액 101,000 (15%)
Each
수량:
1 Kit
제품 가격(KRW)
574,000
온라인 행사
Ends: 31-Mar-2026
675,000할인액 101,000 (15%)
Each
The Calcium Calibration Buffer Kits are designed to help determine the dissociation constant (Kd) of fluorescent Ca2+ indicators using a fluorometer or quantitative imaging system. The Kd of an indicator can be calculated from a plot generated by scanning the excitation or emission of the indicator in the presence of eleven different Ca2+ concentrations. Calcium Calibration Kit #1 contains 50 mL of 10 mM K2EGTA and 50 mL of 10 mM CaEGTA. Both solutions contain 100 mM KCl and 30 mM MOPS, pH 7.2 and are prepared in deionized water. These stock solutions can be blended to make buffers having free Ca2+ ranging from 0 μM to 39 μM.
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법Fluorescence
염료 유형Other Label(s) or Dye(s)
형식Tube(s), Slide(s)
수량1 Kit
배송 조건Room Temperature
용도(애플리케이션)Calcium Calibration
용도 (장비)Fluorescence Microscope, Fluorometer
제품 유형Calcium Calibration Buffer Kit
Unit SizeEach
구성 및 보관
Store in refrigerator at 2°C to 8°C
인용 및 참조 문헌 (66)
인용 및 참조 문헌
Abstract
Mechanism of collagen activation in human platelets.
Journal:J Biol Chem
PubMed ID:14981087
The mechanism of collagen-induced human platelet activation was examined using Ca2+, Na+, and the pH-sensitive fluorescent dyes calcium green/fura red, sodium-binding benzofuran isophthalate, and 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein. Administration of a moderate dose of collagen (10 microg/ml) to human platelets resulted in an increase in [Ca2+](i) and platelet aggregation. The majority of this
The molecular basis of differential subcellular localization of C2 domains of protein kinase C-alpha and group IVa cytosolic phospholipase A2.
Journal:J Biol Chem
PubMed ID:12531893
'The C2 domain is a Ca(2+)-dependent membrane-targeting module found in many cellular proteins involved in signal transduction or membrane trafficking. C2 domains are unique among membrane targeting domains in that they show a wide range of lipid selectivity for the major components of cell membranes, including phosphatidylserine and phosphatidylcholine. To
Differential regulation of nuclear and cytosolic Ca2+ in HeLa cells.
Journal:J Biol Chem
PubMed ID:8940122
'The results reported in this study address the controversial issue that nuclear free Ca2+ ([Ca2+]n) may be regulated independently of cytosolic free Ca2+ ([Ca2+]c). We have measured [Ca2+]n and [Ca2+]c with recombinant aequorin targeted to the nucleus and cytosol in HeLa cells. We found that histamine, ATP, and ionomycin increased
A single optical fiber fluorometric device for measurement of intracellular Ca2+ concentration: its application to hippocampal neurons in vitro and in vivo.
Journal:Neuroscience
PubMed ID:1436506
'We developed a new system to measure the intracellular Ca2+ concentration in the deep region of the central nervous system with a single optical fiber (300 microns in diameter), used for both excitation and detection of the fluorescence of previously loaded fura-2. With this system, a brain region loaded with
Protein and acidosis alter calcium-binding and fluorescence spectra of the calcium indicator indo-1.
Journal:Biophys J
PubMed ID:7819496
'The fluorescent indicator indo-1 is widely used to monitor intracellular calcium concentration. However, quantitation is limited by uncertain effects of the intracellular environment on indicator properties. The goal of this study was to determine the effects of protein and acidosis on the fluorescence spectra and calcium dissociation constant (Kd) of