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인용 및 참조 문헌 (7)
Invitrogen™
Click-IT™ ManNAz Metabolic Glycoprotein Labeling Reagent (tetraacetylated N-Azidoacetyl-D-Mannosamine)
Click-iT™ ManNAz metabolic glycoprotein labeling reagent는 세포 표면 sialic acid를 함유한 glycoprotein을 확인하고 특징을 분석하는 간편하고 확실한 두 단계 방법자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| C33366 | 5.2 mg |
카탈로그 번호 C33366
제품 가격(KRW)
595,000
Online offer
Ends: 31-Mar-2026
700,000할인액 105,000 (15%)
Each
수량:
5.2 mg
제품 가격(KRW)
595,000
Online offer
Ends: 31-Mar-2026
700,000할인액 105,000 (15%)
Each
Click-iT™ ManNAz metabolic glycoprotein labeling reagent는 세포 표면 sialic acid를 함유한 glycoprotein을 확인하고 특징을 분석하는 간편하고 확실한 두 단계 방법 중 첫 부분에 사용합니다. 첫 단계에서 배양 세포가 azide-modified mannosamine (ManNAz)에서 배양됩니다. azido-sugar가 oligosaccharide 생합성 경로의 투과 속성으로 세포 표면 sialic acid 함유 glycoprotein에 대사적으로 통합됩니다. 두 번째 단계에서 azide과 alkyne 간의 chemoselective ligation 또는 click reaction으로 azido로 표지된 glycoprotein을 Click-iT™ Glycoprotein Detection kit로 검출할 수 있습니다[gels (TAMRA 또는 Dapoxyl™ alkyne) 또는 Western blots (biotin alkyne)]. 이 Click-iT™ 제품은 LC-MS⁄MS 및 Multiplexed Proteomics™ 기술과 함께 사용해 심도 깊은 glycoproteome 분석을 할 수 있습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법Biotin-based, Fluorescence
그린 기능Less hazardous
제품라인Click-iT
제품 유형Labeling Reagent
수량5.2 mg
배송 조건Room Temperature
Labeling TargetProteins
라벨 또는 염료Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647, Biotin, Oregon Green 488, TMR (Tetramethylrhodamine)
Unit SizeEach
구성 및 보관
Store in freezer -5°C to -30°C.
인용 및 참조 문헌 (7)
인용 및 참조 문헌
Abstract
Metabolic labeling of glycans with azido sugars for visualization and glycoproteomics.
Journal:Methods Enzymol
PubMed ID:17116478
'The staggering complexity of glycans renders their analysis extraordinarily difficult, particularly in living systems. A recently developed technology, termed metabolic oligosaccharide engineering, enables glycan labeling with probes for visualization in cells and living animals, and enrichment of specific glycoconjugate types for proteomic analysis. This technology involves metabolic labeling of glycans
Copper-free click chemistry for highly luminescent quantum dot conjugates: application to in vivo metabolic imaging.
Journal:Bioconjug Chem
PubMed ID:20222737
Quantum dots (QD) are inorganic nanocrystals with outstanding optical properties, specially suited for biological imaging applications. Their attachment to biomolecules in mild aqueous conditions for the design of bioconjugates is therefore highly desirable. 1,3-dipolar [3 + 2] cycloaddition between azides and terminal alkynes (
Dynamic monitoring of newly synthesized proteomes: up-regulation of myristoylated protein kinase A during butyric acid induced apoptosis.
Journal:Angew Chem Int Ed Engl
PubMed ID:21678537
Doubly charged: A double metabolic incorporation approach capable of proteome-wide profiling of post-translational modification dynamics on newly synthesized proteins has been developed (see scheme; blue box: methionine surrogate, orange diamond: PTM probe). This strategy reveals for the first time that up-regulation of myristoylated PKA protein is necessary for the occurrence
Sialic Acids Attached to O-Glycans Modulate Voltage-gated Potassium Channel Gating.
Journal:J Biol Chem
PubMed ID:21115483
Neuronal, cardiac, and skeletal muscle action potentials are produced and conducted through the highly regulated activity of several types of voltage-gated ion channels. Voltage-gated potassium (K(v)) channels are responsible for action potential repolarization. Glycans can be attached to glycoproteins through N- and O-linkages. Previous reports described the impact of N-glycans
A FRET-based fluorogenic phosphine for live-cell imaging with the Staudinger ligation.
Journal:Angew Chem Int Ed Engl
PubMed ID:18306205
Fluorescent phosphine probes have been used for direct imaging of various azide-bearing biomolecules with the Staudinger ligation in cell-free environments. Recently, we applied phosphine-based dyes to image azides on the surface of live cells. Notably, significant labeling above background could only be achieved using a highly negatively charged