Click-IT™ Tetramethylrhodamine (TAMRA) Protein Analysis Detection Kit

The Click-iT™ Tetramethylrhodamine (TAMRA) Glycoprotein Detection Kit provides the second part of the simple and robust two-step technique to identify and characterize glycoproteins by one- or two-dimensional gel electrophoresis. In step two, after the incorporation of the azide handle into protein glycan structures with either a Click-iT™ metabolic labeling reagent or the Click-iT™ Enzymatic Labeling system, the azide-modified glycoproteins are detected via the chemoselective ligation or click reaction between an azide and an alkyne. Gels with TAMRA labeled glycoproteins can be subsequently stained with the Multiplexed Proteomics™ technologies, SYPRO™ Ruby total protein stain and PRO-Q™ Emerald glycoprotein stain for the differential analysis of glycoprotein subclasses, total proteins and total glycoproteins in the same gel. Click-iT™ modified glycoproteins are also compatible with downstream LC-MS/MS and MALDI MS analysis for identification.