Cholera Toxin Subunit B (Recombinant) Conjugate

인용 및 참조 문헌 (15)

Invitrogen™

Cholera Toxin Subunit B (Recombinant) Conjugate

Name: Cholera Toxin Subunit B (Recombinant) Conjugate
SKU: C34779
Price: 411000 KRW

Molecular Probes™ cholera toxin conjugate는 B subunit의 재조합 버전으로만 만들어집니다. 이를 통해 toxic A subunit이 완전히 제거된 순도가 높은 제품을자세히 알아보기

보기 방식 변경

카탈로그 번호	콘주게이트	수량
C34779	Biotin	100 μg
C22841	Alexa Fluor 488	500 μg
C34776	Alexa Fluor 555	100 μg
C22843	Alexa Fluor 555	500 μg
C34777	Alexa Fluor 594	100 μg
C22842	Alexa Fluor 594	500 μg
C34778	Alexa Fluor 647	100 μg
C34780 C-34780으로도 사용됨	HRP (Horseradish Peroxidase)	100 μg
C34775	Alexa Fluor 488	100 μg

9 개 옵션

카탈로그 번호 C34779

제품 가격(KRW)

411,000

キャンペーン価格

Ends: 31-Dec-2026

483,000

할인액 72,000 (15%)

Each

콘주게이트:

Biotin

수량:

100 μg

제품 가격(KRW)

411,000

キャンペーン価格

Ends: 31-Dec-2026

483,000

할인액 72,000 (15%)

Each

Molecular Probes™ cholera toxin conjugate는 B subunit의 재조합 버전으로만 만들어집니다. 이를 통해 toxic A subunit이 완전히 제거된 순도가 높은 제품을 만들 수 있습니다. Cholera toxin B subunit (CT-B)은 ganglioside G_M1에 결합해 세포에 부착하여, 신경세포의 강력한 역행(retrograde) 표지 도구가 됩니다. 이 tracer는 래트 전뇌 구심성신경(rat forebrain afferent), 부완 영역(parabrachial region) 돌기, 방광벽 신경세포 추적 등 다양한 어플리케이션에 사용되어 왔습니다. 신경세포 추적 어플리케이션에서 CT-B는 대개 신경 조직에 압력 주입(pressure injection) 또는 이온영동 주입(iontophoretic injection)으로 도입됩니다.

Cholera Toxin Subunit A 사양:
• Label (Ex/Em): Alexa Fluor™ 488 (495/519 nm)
• 중성 pH에서 11.4 kDa B subunit이 57 kDa pentamer으로 존재합니다.
• 동결건조 제품을 buffer (예: PBS)에 용해시켜 사용할 수 있습니다.

Cholera Toxin Subunit A의 lipid raft 연구에 사용
최근 연구자들은 세포 신호 전달에 중요하다고 여겨지는 콜레스테롤과 스핑고지질(sphingolipid)에 많은 막 미세영역인 lipid raft 의 표지자로 CT-B를 사용할 수 있다는 사실을 확인했습니다. Lipid raft 염색을 위해 세포를 먼저 형광 CT-B로 배양한 후, anti–CT-B 항체를 가하여 lipid raft 내 CT-B를 세포막 상의 뚜렷한 패치로 교차결합시킵니다. 이들 패치는 형광 현미경검사로 쉽게 확인됩니다. 개별 형광 CT-B conjugate 외에 CT-B의 dye conjugate인 Alexa Fluor™ 488, Alexa Fluor™ 555, 또는 Alexa Fluor™ 594, anti–CT-B antibody, 형광 현미경검사를 위한 세포 표지 및 제조 프로토콜이 들어있는 Vybrant™ Lipid Raft Labeling Kit도 제공합니다.

CT-B Conjugate에 대해 더 알아보기
자사에서는 다양한 CT-B conjugate를 제공하고 있습니다. 이들 tracer에 대한 추가 정보는 Molecular Probes™ Handbook에서 Protein Conjugates—14.7항을 참조하십시오.

연구용으로만 사용할 수 있습니다. 사람이나 동물의 치료 또는 진단 목적으로 사용할 수 없습니다.

For Research Use Only. Not for use in diagnostic procedures.

사양

라벨 유형Biotin & Other Haptens

단백질 형태Recombinant

단백질 아형Cholera Toxin

수량100 μg

배송 조건Room Temperature

콘주게이트Biotin

형태Lyophilized

재조합Recombinant

Unit SizeEach

구성 및 보관

Store in freezer (-5 to -30°C).

자주 묻는 질문(FAQ)

I injected a fluorescent tracer, but cannot detect it after tissue is fixed and sectioned. What am I doing wrong?

Confirm that the tracer you are using crosslinks to proteins or has a primary amine for fixation-either a hydrazide, lysine fixable dextran, or a protein conjugate.
Use aldehyde-based fixatives to cross link the amines on the tracer.
Inject a larger amount or higher concentration of the tracer. Tracers are generally injected at 1-20% concentrations (10 mg/mL or higher).
Confirm that you are using the correct fluorescent filter for detection. You can perform a spot test by pipetting a small amount of the undiluted stock solution of the tracer onto a slide, then view under the filter you are using on your microscope. This will confirm if the tracer fluorescence can be detected and the fluorescent microscope filter is working properly.
Review tissue fixation and handling procedures to confirm if any reagents or processing procedures could be affecting the tracer.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you have a tracer that will only transport retrograde?

Wheat germ agglutinin and cholera toxin conjugates have been used for retrograde tracing. They may have some anterograde tracing in some applications. A selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing/protein-conjugates.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I know which tracer to choose for my experiment?

Factors to consider are size of tracer, method of delivery (injection, direct application to tissue, etc.), and if the tracer needs to be fixable. Here are some links to details about the various classes of neuronal tracers we offer and how to choose between them:

Neuronal Tracing (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html)
Choosing a Tracer (https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/fluorescent-tracers-of-cell-morphology-and-fluid-flow/choosing-a-tracer.html)
Imaging Analysis (http://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/bioprobes-50-journal.pdf)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What products do you have for neuronal tracing?

Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (15)

인용 및 참조 문헌

Abstract

Single molecule analysis of serotonin transporter regulation using antagonist-conjugated quantum dots reveals restricted, p38 MAPK-dependent mobilization underlying uptake activation.

Authors:Chang JC, Tomlinson ID, Warnement MR, Ustione A, Carneiro AM, Piston DW, Blakely RD, Rosenthal SJ,

Journal:J Neurosci

PubMed ID:22745492

'The presynaptic serotonin (5-HT) transporter (SERT) is targeted by widely prescribed antidepressant medications. Altered SERT expression or regulation has been implicated in multiple neuropsychiatric disorders, including anxiety, depression and autism. Here, we implement a generalizable strategy that exploits antagonist-conjugated quantum dots (Qdots) to monitor, for the first time, single SERT ... More

The formation of acetylcholine receptor clusters visualized with quantum dots.

Authors:Geng L, Zhang HL, Peng HB,

Journal:BMC Neurosci

PubMed ID:19604411

BACKGROUND: Motor innervation of skeletal muscle leads to the assembly of acetylcholine receptor (AChR) clusters in the postsynaptic membrane at the vertebrate neuromuscular junction (NMJ). Synaptic AChR aggregation, according to the diffusion-mediated trapping hypothesis, involves the establishment of a postsynaptic scaffold that ... More

Co-localization of amyloid beta and tau pathology in Alzheimer's disease synaptosomes.

Authors:Fein JA, Sokolow S, Miller CA, Vinters HV, Yang F, Cole GM, Gylys KH,

Journal:Am J Pathol

PubMed ID:18467692

The amyloid cascade hypothesis proposes that amyloid beta (Abeta) pathology precedes and induces tau pathology, but the neuropathological connection between these two lesions has not been demonstrated. We examined the regional distribution and co-localization of Abeta and phosphorylated tau (p-tau) in synaptic terminals of Alzheimer's disease brains. To quantitatively examine ... More

A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale.

Authors:Bohland JW, Wu C, Barbas H, Bokil H, Bota M, Breiter HC, Cline HT, Doyle JC, Freed PJ, Greenspan RJ, Haber SN, Hawrylycz M, Herrera DG, Hilgetag CC, Huang ZJ, Jones A, Jones EG, Karten HJ, Kleinfeld D, Kötter R, Lester HA, Lin JM, Mensh BD, Mikula S, Panksepp J, Price JL, Safdieh J, Saper CB, Schiff ND, Schmahmann JD, Stillman BW, Svoboda K, Swanson LW, Toga AW, Van Essen DC, Watson JD, Mitra PP,

Journal:PLoS Comput Biol

PubMed ID:19325892

In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale ... More

Cholera toxin B subunit binding does not correlate with GM1 expression: a study using mouse embryonic neural precursor cells.

Authors:Yanagisawa M, Ariga T, Yu RK

Journal:Glycobiology

PubMed ID:16964630

Gangliosides, sialic acid-containing glycosphingolipids, are ubiquitously expressed in all eukaryotic cells and are primarily localized in the plasma membrane. Cholera toxin B subunit (Ctxb), a component of a heat-labile enterotoxin produced by Vibrio cholerae, has been frequently used as a probe to detect GM1 ganglioside because of its high affinity ... More

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